Human 4-1BB Ligand/TNFSF9 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2295P
Detection of 4‑1BB Ligand/TNFSF9 in Raji Human Cell Line by Flow Cytometry.
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Human 4-1BB Ligand/TNFSF9 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human 4‑1BB Ligand/TNFSF9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB Ligand, recobinant human (rh) APRIL, rhBAFF, rmEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, rhTRANCE, or rhTWEAK is observed.
Source
Monoclonal Mouse IgG2B Clone # 282220
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human 4‑1BB Ligand/TNFSF9
Arg71-Glu254
Accession # P41273.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of 4‑1BB Ligand/TNFSF9 in Raji Human Cell Line by Flow Cytometry. View Larger

Detection of 4‑1BB Ligand/TNFSF9 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human
4‑1BB Ligand/TNFSF9 PE‑conjugated Monoclonal Antibody (Catalog # FAB2295P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL; also CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) of molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A bioactive 26 kDa soluble form of 4-1BBL, presumably generated by MMP cleavage, occurs in humans (4). Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha ) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4+ T cells, and IFN-gamma in CD8+ T cells (9, 10).

References
  1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8.
  2. Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348.
  3. Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 10:481.
  4. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  5. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  6. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  7. Michel, J. et al. (1999) Immunology 98:42.
  8. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  9. Wen, T. et al. (2002) J. Immunol. 168:4897.
  10. Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.
Entrez Gene IDs
8744 (Human); 21950 (Mouse); 353218 (Rat)
Alternate Names
41BB Ligand; 4-1BB Ligand; 4-1BBL; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9

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