Human 4-1BB Ligand/TNFSF9 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human 4‑1BB Ligand/TNFSF9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB Ligand, recombinant human (rh) APRIL, rhBAFF, rmEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, rhTRANCE, or rhTWEAK is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 282220
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human 4‑1BB Ligand/TNFSF9
    Accession # P41273.1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of 4‑1BB Ligand/TNFSF9 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human 4‑1BB Ligand/TNFSF9 APC‑conjugated Monoclonal Antibody (Catalog # FAB2295A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL; also TNFSF9 and CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A bioactive 26 kDa soluble form of 4-1BBL, presumably generated by MMP cleavage, occurs in humans (4). Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha ) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4+ cells, and IFN-gamma in CD8+ cells (9, 10).

  • References:
    1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8.
    2. Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348.
    3. Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 1:481.
    4. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
    5. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
    6. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
    7. Michel, J. et al. (1999) Immunology 98:42.
    8. Langstein, J. et al. (1998) J. Immunol. 160:2488.
    9. Wen, T. et al. (2002) J. Immunol. 168:4897.
    10. Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.
  • Entrez Gene IDs:
    8744 (Human); 21950 (Mouse); 353218 (Rat)
  • Alternate Names:
    41BB Ligand; 4-1BB ligand; 4-1BBL; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9
Related Research Areas
Isotype Controls
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Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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