Human ABCG2 APC-conjugated Antibody

(4 citations)   
  • Species Reactivity
  • Specificity
    Detects human ABCG2 in flow cytometry and immunocytochemistry.
  • Source
    Monoclonal Mouse IgG2B Clone # 5D3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    3T3 cells transduced with human ABCG2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ABCG2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ABCG2 APC‑conjugated Monoclonal Antibody (Catalog # FAB995A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ABCG2
Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface (6).
  • References:
    1. Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
    2. Sorrentino, B.P. et al. (1995) Blood 86:491.
    3. Pallis, M. and N. Russell (2000) Blood 95:2897.
    4. Johnstone, R.W. et al. (1999) Blood 93:1075.
    5. Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
    6. Zhou, S. et al. (2001) Nat. Medicine 7:1028.
    7. Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
    8. Bunting, K.D. (2002) Stem Cells 20:11.
  • Long Name:
    ATP-binding Cassette Transporter G2
  • Entrez Gene IDs:
    9429 (Human)
  • Alternate Names:
    ABC transporter; ABC15; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 4 of 4
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Sample Type
  1. Stem cell-like side populations in esophageal cancer: a source of chemotherapy resistance and metastases.
    Authors: Zhao Y, Bao Q, Schwarz B, Zhao L, Mysliwietz J, Ellwart J, Renner A, Hirner H, Niess H, Camaj P, Angele M, Gros S, Izbicki J, Jauch K, Nelson P, Bruns C
    Stem Cells Dev, 2014;23(2):180-92.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  2. MDR1 expression identifies human melanoma stem cells.
    Authors: Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G
    Biochem. Biophys. Res. Commun., 2008;368(4):930-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter.
    Authors: Ozvegy-Laczka C, Laczko R, Hegedus C, Litman T, Varady G, Goda K, Hegedus T, Dokholyan NV, Sorrentino BP, Varadi A, Sarkadi B
    J. Biol. Chem., 2008;283(38):26059-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. C421 allele-specific ABCG2 gene amplification confers resistance to the antitumor triazoloacridone C-1305 in human lung cancer cells.
    Authors: Bram EE, Ifergan I, Grimberg M, Lemke K, Skladanowski A, Assaraf YG
    Biochem. Pharmacol., 2007;74(1):41-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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