Human ABCG2 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ABCG2 in flow cytometry and immunocytochemistry.
  • Source
    Monoclonal Mouse IgG2B Clone # 5D3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    3T3 cells transduced with human ABCG2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human ABCG2 transfected CHO-S cells
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
ABCG2 in JAR Human Cell Line. ATP-Binding Cassette Transporter G2 (ABCG2) was detected in immersion fixed JAR human choriocarcinoma cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
ABCG2 in Human ABCG2 transfected CHO Chinese Hamster Cell Line. ABCG2 was detected in immersion fixed Human ABCG2 transfected CHO Chinese hamster ovary cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ABCG2
Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface (6).
  • References:
    1. Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
    2. Sorrentino, B.P. et al. (1995) Blood 86:491.
    3. Pallis, M. and N. Russell (2000) Blood 95:2897.
    4. Johnstone, R.W. et al. (1999) Blood 93:1075.
    5. Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
    6. Zhou, S. et al. (2001) Nat. Medicine 7:1028.
    7. Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
    8. Bunting, K.D. (2002) Stem Cells 20:11.
  • Long Name:
    ATP-binding Cassette Transporter G2
  • Entrez Gene IDs:
    9429 (Human)
  • Alternate Names:
    ABC transporter; ABC15; ABCG2; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling
    Authors: K Yamamizu, M Iwasaki, H Takakubo, T Sakamoto, T Ikuno, M Miyoshi, T Kondo, Y Nakao, M Nakagawa, H Inoue, JK Yamashita
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. The Metastatic Potential and Chemoresistance of Human Pancreatic Cancer Stem Cells.
    Authors: Bhagwandin V, Bishop J, Wright W, Shay J
    PLoS ONE, 2015;11(2):e0148807.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Mitochondrial localization of ABC transporter ABCG2 and its function in 5-aminolevulinic acid-mediated protoporphyrin IX accumulation.
    Authors: Kobuchi, Hirotsug, Moriya, Koko, Ogino, Tetsuya, Fujita, Hirofumi, Inoue, Keiji, Shuin, Taro, Yasuda, Tatsuji, Utsumi, Kozo, Utsumi, Toshihik
    PLoS ONE, 2012;7(11):e50082.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression.
    Authors: Ji F, Wang Y, Sun H, Du J, Zhao H, Wang D, Xu Q, Duan D, Yang H
    J. Neuroimmunol., 2008;197(2):99-109.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2007;319(1):1-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea.
    Authors: Shimmura S, Nagoshi N, Matsuzaki Y, Okano H, Tsubota K
    Stem Cells, 2006;24(12):2714-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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