Human Acetylcholinesterase/ACHE Quantikine ELISA Kit

R&D Systems | Catalog # DACHE0

Please consider catalog # DY7574-05 as an alternative.
R&D Systems
Discontinued Product
DACHE0 has been discontinued. View all Acetylcholinesterase/ACHE products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

43.7 pg/mL

Assay Range

125-8000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
View all Acetylcholinesterase/ACHE ELISA Kits »

Product Summary for Human Acetylcholinesterase/ACHE Quantikine ELISA Kit

The Quantikine Human Acetylcholinesterase/ACHE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACHE levels in cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant human ACHE and antibodies raised against the recombinant protein. Results obtained for naturally occurring human ACHE showed linear curves that were parallel to the standard curves obtained using the Quantikine Human ACHE Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human ACHE.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human ACHE.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 476 1333 2646 482 1353 2731
Standard Deviation 14.6 44.4 222 34.3 79.3 81.7
CV% 3.1 3.3 8.4 7.1 5.9 3.0

Recovery for Human Acetylcholinesterase/ACHE Quantikine ELISA Kit

The recovery of human ACHE spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 87-113
Cell Lysis Buffer (n=4) 99 86-111
EDTA Plasma (n=4) 102 93-119
Heparin Plasma (n=4) 95 84-110
Serum (n=4) 101 95-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ACHE were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Acetylcholinesterase/ACHE ELISA Linearity

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Acetylcholinesterase/ACHE

The classical role of ACHE is to terminate cholinergic neurotransmission by hydrolysis of acetylcholine (ACH). ACHE is thought to be involved in the pathology of Alzheimer's disease (AD) by accelerating the assembly of Abeta peptides into fibrillar species through forming complexes with Abeta via the peripheral anionic site on ACHE. ACHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance ACH availability, as well as reduce amyloidogenesis and subsequent neurotoxicity. Its involvement in the cholinergic anti-inflammatory pathway connects ACHE with a possible marker of low-grade systemic inflammation in obesity, hypertension, coronary heart disease, and AD. Alternative splicing produces three isoforms: an amphipathic form that exists as both monomeric and mutimeric forms, a soluble-monomeric form lacking the cysteine residue near the C-terminus, and a GPI-anchored dimeric form found in the membranes of erythrocytes. The recombinant mouse ACHE (rmACHE) was expressed as the amphipathic form that consists of soluble monomer and mutimeric forms.

Alternate Names

ACHE, ARACHE, N-ACHE

Entrez Gene IDs

43 (Human); 11423 (Mouse); 83817 (Rat)

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Human Acetylcholinesterase/ACHE Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Acetylcholinesterase/ACHE Quantikine ELISA Kit

For research use only

Citations for Human Acetylcholinesterase/ACHE Quantikine ELISA Kit

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