Human Acetylcholinesterase/ACHE Quantikine ELISA Kit

DACHE0 has been discontinued. View all Acetylcholinesterase/ACHE products.
Please consider catalog # DY7574-05 as an alternative.
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    43.7 pg/mL
  • Assay Range
    125.0 - 8,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human ACHE.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Acetylcholinesterase/ACHE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACHE levels in cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant human ACHE and antibodies raised against the recombinant protein. Results obtained for naturally occurring human ACHE showed linear curves that were parallel to the standard curves obtained using the Quantikine Human ACHE Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human ACHE.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean4761333264648213532731
Standard Deviation14.644.422234.379.381.7
CV%3.13.38.47.15.93

Recovery

The recovery of human ACHE spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 87-113
Cell Lysis Buffer (n=4) 99 86-111
EDTA Plasma (n=4) 102 93-119
Heparin Plasma (n=4) 95 84-110
Serum (n=4) 101 95-114
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ACHE were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Acetylcholinesterase/ACHE Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Acetylcholinesterase/ACHE
The classical role of ACHE is to terminate cholinergic neurotransmission by hydrolysis of acetylcholine (ACH). ACHE is thought to be involved in the pathology of Alzheimer's disease (AD) by accelerating the assembly of Abeta peptides into fibrillar species through forming complexes with Abeta via the peripheral anionic site on ACHE. ACHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance ACH availability, as well as reduce amyloidogenesis and subsequent neurotoxicity. Its involvement in the cholinergic anti-inflammatory pathway connects ACHE with a possible marker of low-grade systemic inflammation in obesity, hypertension, coronary heart disease, and AD. Alternative splicing produces three isoforms: an amphipathic form that exists as both monomeric and mutimeric forms, a soluble-monomeric form lacking the cysteine residue near the C-terminus, and a GPI-anchored dimeric form found in the membranes of erythrocytes. The recombinant mouse ACHE (rmACHE) was expressed as the amphipathic form that consists of soluble monomer and mutimeric forms.
    • Entrez Gene IDs
      43 (Human); 11423 (Mouse); 83817 (Rat);
    • Alternate Names
      acetylcholinesterase (Yt blood group); Acetylcholinesterase; ACHE; apoptosis-related acetylcholinesterase; ARACHE; EC 3.1.1; EC 3.1.1.7; N-ACHE; Yt blood group; YT;
    Related Research Areas

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