Human Acetylcholinesterase/ACHE Quantikine ELISA Kit
Human Acetylcholinesterase/ACHE Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 476 | 1333 | 2646 | 482 | 1353 | 2731 |
| Standard Deviation | 14.6 | 44.4 | 222 | 34.3 | 79.3 | 81.7 |
| CV% | 3.1 | 3.3 | 8.4 | 7.1 | 5.9 | 3 |
Recovery
The recovery of human ACHE spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 87-113 |
| Cell Lysis Buffer (n=4) | 99 | 86-111 |
| EDTA Plasma (n=4) | 102 | 93-119 |
| Heparin Plasma (n=4) | 95 | 84-110 |
| Serum (n=4) | 101 | 95-114 |
Linearity
Product Datasheets
Preparation and Storage
Background: Acetylcholinesterase/ACHE
The classical role of ACHE is to terminate cholinergic neurotransmission by hydrolysis of acetylcholine (ACH). ACHE is thought to be involved in the pathology of Alzheimer's disease (AD) by accelerating the assembly of Abeta peptides into fibrillar species through forming complexes with Abeta via the peripheral anionic site on ACHE. ACHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance ACH availability, as well as reduce amyloidogenesis and subsequent neurotoxicity. Its involvement in the cholinergic anti-inflammatory pathway connects ACHE with a possible marker of low-grade systemic inflammation in obesity, hypertension, coronary heart disease, and AD. Alternative splicing produces three isoforms: an amphipathic form that exists as both monomeric and mutimeric forms, a soluble-monomeric form lacking the cysteine residue near the C-terminus, and a GPI-anchored dimeric form found in the membranes of erythrocytes. The recombinant mouse ACHE (rmACHE) was expressed as the amphipathic form that consists of soluble monomer and mutimeric forms.
Citation for Human Acetylcholinesterase/ACHE Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Serum Level and Activity of Butylcholinesterase: A Biomarker for Post-Stroke Dementia
Authors: YC Chen, WH Chou, CP Fang, TH Liu, HH Tsou, Y Wang, YL Liu
J Clin Med, 2019-10-24;8(11):.
Species: Human
Sample Types: Plasma
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsReviews for Human Acetylcholinesterase/ACHE Quantikine ELISA Kit
There are currently no reviews for this product. Be the first to review Human Acetylcholinesterase/ACHE Quantikine ELISA Kit and earn rewards!
Have you used Human Acetylcholinesterase/ACHE Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
