Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ADAM23 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM23
Ser60-His585
Accession # O75077

Specificity

Detects human ADAM23 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) ADAM8, rhADAM9, and rhADAM22 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human ADAM23 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM23, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAM23

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM23

ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100 kDa member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter‑receptor for alpha v beta 3 integrin. The human ADAM23 proprecursor is a 773 amino acid (aa) type I transmembrane (TM) protein. It contains a 227 aa cleavable proregion (aa 60‑286) and a 506 aa extracellular domain (ECD) (aa 287‑792) that is part of a 70 kDa mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299‑494), an integrin‑binding disintegrin region (aa 511‑585) and a Cys‑rich domain (aa 589‑611). Two splice variants exist. One shows an in‑frame 46 aa substitution for aa 787‑832 that generates a soluble form, while a second shows an in‑frame 31 aa TM substitution for aa 787‑817. Over aa 1‑585, human ADAM23 is 92% aa identical to mouse ADAM23.

Long Name

A Disintegrin and Metalloproteinase Domain 23

Alternate Names

MDC-L, MDC3

Entrez Gene IDs

8745 (Human); 23792 (Mouse); 301460 (Rat)

Gene Symbol

ADAM23

UniProt

O75077

Additional ADAM23 Products

Product Documents for Human ADAM23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ADAM23 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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