Human Akt1 PE-conjugated Antibody

IC17751P has been discontinued. View all Akt1 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Akt1 in direct ELISAs. Reactivity with Akt1 from other species has not been determined. In direct ELISAs, this antibody does not detect recombinant human (rh) Akt2 or rhAkt3.
  • Source
    Monoclonal Mouse IgG1 Clone # 302407
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt1
    Ser2-Ala480
    Accession # P31749
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Akt1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Akt1 PE‑conjugated Monoclonal Antibody (Catalog # IC17751P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Akt1

Akt, also known as Protein Kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

  • Long Name:
    v-Akt Murine Thymoma Viral Oncogene Homolog 1
  • Entrez Gene IDs:
    207 (Human); 11651 (Mouse); 25233 (Rat)
  • Alternate Names:
    AKT; Akt1; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKBMGC99656; PRKBA; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; v-akt murine thymoma viral oncogene homolog 1
Related Research Areas

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