Human ALCAM/CD166 PE-conjugated Antibody

(10 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ALCAM in Western blots. Shows approximately 50% cross-reactivity with recombinant mouse OCAM and no cross-reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.
  • Source
    Monoclonal Mouse IgG1 Clone # 105902
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
    Trp28-Ala526
    Accession # Q13740
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ALCAM/CD166 in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human ALCAM/CD166 PE‑conjugated Monoclonal Antibody (Catalog # FAB6561P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

  • References:
    1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
    2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
    3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  • Long Name:
    Activated Leukocyte Cell Adhesion Molecule
  • Entrez Gene IDs:
    214 (Human); 11658 (Mouse)
  • Alternate Names:
    activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas
    Stem Cell Reports, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Establishment of highly tumorigenic human colorectal cancer cell line (CR4) with properties of putative cancer stem cells.
    Authors: Rowehl, Rebecca, Burke, Stephani, Bialkowska, Agnieszk, Pettet, Donald W, Rowehl, Leahana, Li, Ellen, Antoniou, Eric, Zhang, Yuanhao, Bergamaschi, Roberto, Shroyer, Kenneth, Ojima, Iwao, Botchkina, Galina I
    PLoS ONE, 2014;9(6):e99091.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Activated leukocyte cell adhesion molecule (CD166): an "inert" cancer stem cell marker for non-small cell lung cancer?
    Authors: Tachezy M, Zander H, Wolters-Eisfeld G, Muller J, Wicklein D, Gebauer F, Izbicki J, Bockhorn M
    Stem Cells, 2014;32(6):1429-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells.
    Authors: Fujiwara K, Ohuchida K, Sada M, Horioka K, Ulrich C, Shindo K, Ohtsuka T, Takahata S, Mizumoto K, Oda Y, Tanaka M
    PLoS ONE, 2014;9(9):e107247.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014;9(1):e84417.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012;7(10):e47187.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury.
    Authors: Soh, Boon Sen, Zheng, Dahai, Li Yeo, Julie Su, Yang, Henry He, Ng, Shi Yan, Wong, Lan Hion, Zhang, Wencai, Li, Pin, Nichane, Massimo, Asmat, Atasha, Wong, Poo Sing, Wong, Peng Che, Su, Lin Lin, Mantalaris, Sakis A, Lu, Jia, Xian, Wa, McKeon, Frank, Chen, Jianzhu, Lim, Elaine H, Lim, Bing
    Mol Ther, 2012;20(12):2335-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell selection
  8. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011;6(5):e19988.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.
    Authors: Kondo S, Wakisaka N, Muramatsu M
    J. Virol., 2011;85(21):11255-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
    Authors: Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
    Am. J. Physiol., Cell Physiol., 2010;298(5):C1217-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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