Human ALCAM/CD166 Antibody

Catalog # Availability Size / Price Qty
MAB6561-SP
MAB6561-100
MAB6561-500
Product Details
Citations (8)
FAQs
Supplemental Products
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Human ALCAM/CD166 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALCAM/CD166 in Western blots. Shows approximately 50% cross‑reactivity with recombinant mouse OCAM and no cross‑reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.
Source
Monoclonal Mouse IgG1 Clone # 105902
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # Q13740
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human ALCAM/CD166 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human ALCAM/CD166 Biotinylated Antibody (Catalog # BAF656)

Standard: Recombinant Human ALCAM Fc Chimera Protein, CF (Catalog # 656-AL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

Product Datasheets

Citations for Human ALCAM/CD166 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma
    Authors: U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher
    Cancers (Basel), 2019;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Alternative splicing of ALCAM enables tunable regulation of cell-cell adhesion through differential proteolysis
    Authors: KE Hebron, EY Li, SA Arnold Egl, AK von Lersne, C Taylor, J Houkes, DK Flaherty, A Eskaros, TP Stricker, A Zijlstra
    Sci Rep, 2018;8(1):3208.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
    Authors: Williams D, Anastos K, Morgello S, Berman J
    J Leukoc Biol, 2015;97(2):401-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome.
    Authors: Hansen A, Freeman T, Arnold S, Starchenko A, Jones-Paris C, Gilger M, Washington M, Fan K, Shyr Y, Beauchamp R, Zijlstra A
    Cancer Res, 2013;73(10):2955-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Relevance of activated leukocyte cell adhesion molecule (ALCAM) in tumor tissue and sera of cervical cancer patients.
    Authors: Ihnen M, Kress K, Kersten JF, Kilic E, Choschzick M, Zander H, Muller V, Mahner S, Janicke F, Woelber L, Milde-Langosch K
    BMC Cancer, 2012;12(0):140.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  7. Activated leukocyte cell adhesion molecule: a novel biomarker for breast cancer.
    Authors: Kulasingam V, Zheng Y, Soosaipillai A, Leon AE, Gion M, Diamandis EP
    Int. J. Cancer, 2009;125(1):9-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  8. Phenotypic characterization of human colorectal cancer stem cells.
    Authors: Dalerba P, Dylla S, Park I, Liu R, Wang X, Cho R, Hoey T, Gurney A, Huang E, Simeone D, Shelton A, Parmiani G, Castelli C, Clarke M
    Proc Natl Acad Sci U S A, 0;104(24):10158-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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