Detects human Aldo-keto Reductase 1C3/AKR1C3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) AKR1C1 is observed, and approximately 25% cross-reactivity with rhAKR1C4 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3 Met1-Tyr323 Accession # P42330
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot. Western blot shows lysates of Huh‑7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aldo-keto Reductase 1C3/AKR1C3
AKR1C3 (Aldo-Keto Reductase family 1 member C3; also 17-beta HSD 5, prostaglandin F synthase/PGFS and 3-alpha HSD type 2) is a 35-36 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. It is expressed by multiple cell types, including prostate epithelium, T cells, and hepatocytes. AKR1C3 generates testosterone and progesterone, catalyzes the conversion of aldehydes and ketones into alcohols, and the mediates the reduction of prostaglandin D2 into PGF2. Human AKR1C3 is 323 amino acids (aa) in length. There are three potential isoform variants. One contains an alternative start site at Met120, a second shows a five aa substitution for aa 1-28, and a third possesses a 15 aa substitution for aa 124-323. Full-length human AKR1C3 shares 73% aa sequence identity with mouse AKR1C3.
Aldo-keto Reductase Family 1, Member C3
Entrez Gene IDs:
8644 (Human); 105349 (Mouse); 171516 (Rat)
17-beta-hydroxysteroid dehydrogenase type 5; 3-alpha-HSD type 2; 3-alpha-hydroxysteroid dehydrogenase type 2; AKR1C3; aldo-keto reductase family 1 member C3; aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II); AldoketoReductase 1C3; Chlordecone reductase homolog HAKRb; DD3; DD-3; DD317-beta-HSD 5; DDH1; DDX; Dihydrodiol dehydrogenase 3; Dihydrodiol dehydrogenase type I; dihydrodiol dehydrogenase X; EC 1.1.1; EC 22.214.171.124,3-alpha-HSD type II, brain; EC 126.96.36.199; EC 188.8.131.52; EC 184.108.40.206; EC 220.127.116.11; EC 18.104.22.168; HA1753; HAKRB; HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase; HAKRe; HSD17B5; hydroxysteroid (17-beta) dehydrogenase 5; Indanol dehydrogenase; KIAA0119hluPGFS; PGFS; Prostaglandin F synthase; Testosterone 17-beta-dehydrogenase 5; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
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