Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 388816
View all Alkaline Phosphatase/ALPL Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Alkaline Phosphatase/ALPL
Leu18-Ser502
Accession # P05186

Specificity

Detects human Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human Alkaline Phosphatase/ALPL Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Alkaline Phosphatase/ALPL (Catalog # 2909-AP)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Alkaline Phosphatase/ALPL

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

References

  1. Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
  2. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.

Long Name

Alkaline Phosphatase Liver

Alternate Names

Akp2, AP-TNAP, HOPS, TNAP, TNSALP

Entrez Gene IDs

249 (Human); 11647 (Mouse)

Gene Symbol

ALPL

UniProt

P05186

Additional Alkaline Phosphatase/ALPL Products

Product Documents for Human Alkaline Phosphatase/ALPL Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Alkaline Phosphatase/ALPL Antibody

For research use only

Citations for Human Alkaline Phosphatase/ALPL Antibody

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