Human Alkaline Phosphatase/ALPL Antibody

Catalog # Availability Size / Price Qty
Alkaline Phosphatase/ALPL in BG01V Human Embryonic Stem Cells.
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Product Details
Citations (5)
Supplemental Products
Reviews (3)

Human Alkaline Phosphatase/ALPL Antibody Summary

Species Reactivity
Detects human Alkaline Phosphatase/ALPL in direct ELISAs. In direct ELISAs, 80-100% cross-reactivity with recombinant mouse Alkaline Phosphatase/ALPL is observed and no cross-reactivity with recombinant human (rh) Alkaline Phosphatase/ALPP or rhAlkaline Phosphatase/ALPI is observed.
Monoclonal Rat IgG2A Clone # 388828
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Alkaline Phosphatase/ALPL
Accession # P05186
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Alkaline Phosphatase/ALPL antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Alkaline Phosphatase/ALPL in BG01V Human Embryonic Stem Cells. Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

  1. Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
  2. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Human Alkaline Phosphatase/ALPL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Tissue Nonspecific Alkaline Phosphatase Function in Bone and Muscle Progenitor Cells: Control of Mitochondrial Respiration and ATP Production
    Authors: Z Zhang, HK Nam, S Crouch, NE Hatch
    International Journal of Molecular Sciences, 2021;22(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis
    Authors: R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu
    Nat Commun, 2020;11(1):460.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
    Authors: Onishi K, Tonge P, Nagy A, Zandstra P
    Stem Cell Reports, 2014;3(1):156-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Enzyme replacement prevents enamel defects in hypophosphatasia mice.
    Authors: Yadav MC, de Oliveira RC, Foster BL, Fong H, Cory E, Narisawa S, Sah RL, Somerman M, Whyte MP, Millan JL
    J. Bone Miner. Res., 2012;27(8):1722-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Central role of pyrophosphate in acellular cementum formation.
    Authors: Foster BL, Nagatomo KJ, Nociti FH, Fong H, Dunn D, Tran AB, Wang W, Narisawa S, Millan JL, Somerman MJ
    PLoS ONE, 2012;7(6):e38393.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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Reviews for Human Alkaline Phosphatase/ALPL Antibody

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Human Alkaline Phosphatase/ALPL Antibody
By Mingxia Zhou on 02/16/2020
Application: Flow Sample Tested: activated mouse CD8 T cell Species: Mouse

Human Alkaline Phosphatase/ALPL Antibody
By Anonymous on 02/16/2020
Application: ELISA Sample Tested: Cell Culture Media Species: Human

Human Alkaline Phosphatase/ALPL Antibody
By Anonymous on 10/29/2018
Application: ICC/IF Sample Tested: bone marrow Species: Mouse

Antibody staining was performed on mouse bone marrow sections. Please refer to PMID 29232552 for this staining, experimental details, and other data using this antibody.