Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 388828
View all Alkaline Phosphatase/ALPL Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Alkaline Phosphatase/ALPL
Leu18-Ser502
Accession # P05186

Specificity

Detects human Alkaline Phosphatase/ALPL in direct ELISAs. In direct ELISAs, 80-100% cross-reactivity with recombinant mouse Alkaline Phosphatase/ALPL is observed and no cross-reactivity with recombinant human (rh) Alkaline Phosphatase/ALPP or rhAlkaline Phosphatase/ALPI is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Alkaline Phosphatase/ALPL Antibody

Alkaline Phosphatase/ALPL antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Alkaline Phosphatase/ALPL in BG01V Human Embryonic Stem Cells.

Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Alkaline Phosphatase/ALPL Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Reviewed Applications

Read 3 reviews rated 5 using MAB29091 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Alkaline Phosphatase/ALPL

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

References

  1. Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
  2. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.

Long Name

Alkaline Phosphatase Liver

Alternate Names

Akp2, AP-TNAP, HOPS, TNAP, TNSALP

Entrez Gene IDs

249 (Human); 11647 (Mouse)

Gene Symbol

ALPL

UniProt

P05186

Additional Alkaline Phosphatase/ALPL Products

Product Documents for Human Alkaline Phosphatase/ALPL Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Alkaline Phosphatase/ALPL Antibody

For research use only

Citations for Human Alkaline Phosphatase/ALPL Antibody

Customer Reviews for Human Alkaline Phosphatase/ALPL Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Alkaline Phosphatase/ALPL Antibody
    Name: Mingxia Zhou
    Application: Flow Cytometry
    Sample Tested: activated mouse CD8 T cell
    Species: Mouse
    Verified Customer | Posted 02/16/2020
    Human Alkaline Phosphatase/ALPL Antibody MAB29091
  • Human Alkaline Phosphatase/ALPL Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell Culture Media
    Species: Human
    Verified Customer | Posted 02/16/2020
    Human Alkaline Phosphatase/ALPL Antibody MAB29091
  • Human Alkaline Phosphatase/ALPL Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: bone marrow
    Species: Mouse
    Verified Customer | Posted 10/29/2018
    Antibody staining was performed on mouse bone marrow sections. Please refer to PMID 29232552 for this staining, experimental details, and other data using this antibody.
    Human Alkaline Phosphatase/ALPL Antibody MAB29091

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