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Human Alkaline Phosphatase/ALPL PerCP-conjugated Antibody

Catalog #: FAB1448C Datasheet / COA / SDS

Discontinued Product

FAB1448C has been discontinued.
View all Alkaline Phosphatase/ALPL products.
Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.
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Human Alkaline Phosphatase/ALPL PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Source
Monoclonal Mouse IgG1 Clone # B4-78
Purification
Protein A or G purified from ascites
Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Alkaline Phosphatase/ALPL antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL PerCP-conjugated Monoclonal Antibody (Catalog # FAB1448C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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