Human Amphiregulin Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (5)
Supplemental Products

Human Amphiregulin Biotinylated Antibody Summary

Species Reactivity
Detects human Amphiregulin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with rmAR, rhEGF and rhTGF-alpha is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Amphiregulin
Accession # P15514
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Amphiregulin (Catalog # 262-AR)

Human Amphiregulin Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Amphiregulin Antibody (Catalog # MAB262)

Standard: Recombinant Human Amphiregulin Protein (Catalog # 262-AR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Amphiregulin

Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5 - 10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.

Entrez Gene IDs
374 (Human); 11839 (Mouse)
Alternate Names
Amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor; SDGF

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Citations for Human Amphiregulin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces
    Authors: AM Tsou, H Yano, CN Parkhurst, T Mahlakõiv, C Chu, W Zhang, Z He, KJ Jarick, C Zhong, GG Putzel, M Hatazaki, JRI IBD Li, IC Lorenz, D Andrew, P Balderes, CSN Klose, SA Lira, D Artis
    Nature, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response
    Authors: LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto
    Sci Rep, 2019;9(1):8702.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  3. ADAM17 substrate release in proximal tubule drives kidney fibrosis
    JCI Insight, 2016;1(13):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.
    Authors: Stoll S, Stuart P, Lambert S, Gandarillas A, Rittie L, Johnston A, Elder J
    J Invest Dermatol, 2016;136(2):444-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  5. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development


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