Quick Order  

Welcome to Bio-Techne Quick Order!

Please enter a catalog number:
 
Enter CSV values, as SKU, QUANTITY.
 
View Your Cart

Simplified Shipping


We believe shipping should be simple. We charge a flat rate that depends on your currency and location.
  • No hidden charges
  • No additional charges for dry ice
  • No additional charges for multiple shipments
Choose a shipping destination
Albania

Algeria

Andorra

Angola

Antigua and Barbuda

Argentina

Armenia

Aruba

Australia

Austria

Azerbaijan

Bahamas

Bahrain

Bangladesh

Barbados

Belarus

Belgium

Belize

Benin

Bermuda

Bolivia

Bosnia and Herzegovina

Botswana

Brazil

Brunei

Bulgaria

Burkina Faso

Burundi

Cambodia

Cameroon

Canada

Cayman Islands

Central African Republic

Chad

Chile

China

Colombia

Costa Rica

Ivory Coast

Croatia

Cyprus

Czech Republic

Denmark

Djibouti

Dominica

Dominican Republic

Ecuador

Egypt

El Salvador

Equatorial Guinea

Eritrea

Estonia

Ethiopia

Falkland Islands

Fiji

Finland

France

French Polynesia

Gabon

Gambia

Georgia

Germany

Ghana

Gibraltar

Greece

Grenada

Guadeloupe

Guam

Guatemala

Guinea

Guinea-Bissau

Guyana

Haiti

Vatican

Honduras

Hong Kong S.A.R., China

Hungary

Iceland

India

Indonesia

Iraq

Ireland

Israel

Italy

Jamaica

Japan

Jordan

Kazakhstan

Kenya

South Korea

Kuwait

Kyrgyzstan

Latvia

Lebanon

Lesotho

Liberia

Liechtenstein

Lithuania

Luxembourg

Macedonia

Madagascar

Malawi

Malaysia

Mali

Malta

Marshall Islands

Mauritania

Mexico

Moldova

Monaco

Mongolia

Montenegro

Morocco

Mozambique

Myanmar

Namibia

Nepal

Netherlands

Netherlands Antilles

New Caledonia

New Zealand

Nicaragua

Niger

Nigeria

Northern Mariana Islands

Norway

Oman

Pakistan

Palau

Panama

Papua New Guinea

Paraguay

Peru

Philippines

Poland

Portugal

Puerto Rico

Qatar

Romania

Russia

Rwanda

Saint Kitts and Nevis

Saint Lucia

Saint Martin (French part)

Samoa

Saudi Arabia

Senegal

Serbia

Sierra Leone

Singapore

Slovakia

Slovenia

Solomon Islands

South Africa

Spain

Sri Lanka

Suriname

Swaziland

Sweden

Switzerland

Taiwan

Tanzania

Thailand

Togo

Trinidad and Tobago

Tunisia

Turkey

Uganda

Ukraine

United Arab Emirates

United Kingdom

United States

Uruguay

Uzbekistan

Venezuela

Vietnam

Yemen

Zambia

Zimbabwe

 

OR
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    2.5 hours
  • Sensitivity
    6 pg/mL
  • Assay Range
    78.10 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma,)
  • Specificity
    Natural and recombinant human Angiogenin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Angiogenin Immunoassay is a 2.5 hour solid phase ELISA designed to measure Angiogenin in cell culture supernates, serum, and plasma. It contains recombinant human Angiogenin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate both natural and recombinant human Angiogenin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Inter-Assay Precision (Precision between assays Three samples of known concentration were tested on one plate to assess intra-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean356717173966419113671
Standard Deviation10245047166309
CV%2.83.32.97.18.78.4

Recovery

The recovery of Angiogenin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 97-117
Citrate Plasma (n=10) 102 84-126
EDTA Plasma (n=10) 101 79-121
Heparin Plasma (n=10) 102 80-137
Serum (n=10) 103 84-126
Linearity
The following samples were diluted with Calibrator Diluent and assayed to assess linearity of the assay.
Angiogenin_DAN00_ELISA_28.jpg
Background: Angiogenin
Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 14
    Filter your results:

    Species
    Sample Type
    1. Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer.
      Authors: Landt S, Mordelt K, Schwidde I, Barinoff J, Korlach S, Stoblen F, Lichtenegger W, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2651-5.
      Species: Human
      Sample Type: Serum
    2. Glycemic control influences serum angiogenin concentrations in patients with type 2 diabetes.
      Authors: Siebert J, Reiwer-Gostomska M, Mysliwska J
      Diabetes Care, 2010;33(8):1829-30.
      Species: Human
      Sample Type: Serum
    3. A comparative study of two angiogenic factors: vascular endothelial growth factor and angiogenin in induced sputum from asthmatic children in acute attack.
      Authors: Abdel-Rahman AM, el-Sahrigy SA, Bakr SI
      Chest, 2006;129(2):266-71.
      Species: Human
      Sample Type: Saliva
    4. Plasma angiogenin levels in acute coronary syndromes: implications for prognosis.
      Authors: Tello-Montoliu A, Marin F, Patel J, Roldan V, Mainar L, Vicente V, Sogorb F, Lip GY
      Eur. Heart J., 2007;28(24):3006-11.
      Species: Human
      Sample Type: Plasma
    5. Low serum angiogenin concentrations in patients with type 2 diabetes.
      Authors: Siebert J, Reiwer-Gostomska M, Babinska Z, Mysliwska J, Mysliwski A, Skopinska-Rozewska E, Sommer E, Skopinski P
      Diabetes Care, 2007;30(12):3086-7.
      Species: Human
      Sample Type: Serum
    6. Comparison of cytokeratin 20 RNA and angiogenin in voided urine samples as diagnostic tools for bladder carcinoma.
      Authors: Eissa S, Kenawy G, Swellam M, El-Fadle AA, Abd El-Aal AA, El-Ahmady O
      Clin. Biochem., 2004;37(9):803-10.
      Species: Human
      Sample Type: Urine
    7. Elevated angiogenin levels in the peritoneal fluid of women with endometriosis correlate with the extent of the disorder.
      Authors: Suzumori N, Zhao XX, Suzumori K
      Fertil. Steril., 2004;82(1):93-6.
      Species: Human
      Sample Type: Peritoneal Fluid
    8. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
      Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
      Exp. Hematol., 2007;35(7):1119-31.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
      Authors: Bourlev V, Iljasova N, Adamyan L, Larsson A, Olovsson M
      Fertil. Steril., 2010;94(1):52-7.
    10. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
      Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
      Br. J. Haematol., 2007;137(6):560-8.
      Species: Human
      Sample Type: Serum
    11. Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
      Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
      Leuk. Lymphoma, 2006;47(6):1034-40.
      Species: Human
      Sample Type: Serum
    12. Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor κB and miR-221.
      Authors: Santhekadur PK, Das SK, Gredler R, Chen D, Srivastava J, Robertson C, Baldwin AS, Fisher PB, Sarkar D
      J. Biol. Chem., 2012;287(17):13952-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
      Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
      Diabetologia, 2011;54(9):2358-67.
      Species: Human
      Sample Type: Serum
    14. Plasma haemoxygenase-1 in coronary artery disease. A comparison with angiogenin, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 and vascular endothelial growth factor.
      Authors: Idriss NK, Lip GY, Balakrishnan B
      Thromb. Haemost., 2010;104(5):1029-37.
      Species: Human
      Sample Type: Plasma
    Expand to show all

    FAQs

    No product specific FAQs exist for this product, however you may click here to view all FAQs for ELISAs

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest

    Feedback

    Customer Information