Human Angiogenin Quantikine ELISA Kit

(15 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    2.5 hours
  • Sensitivity
    6 pg/mL
  • Assay Range
    78.10 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma,)
  • Specificity
    Natural and recombinant human Angiogenin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Angiogenin Immunoassay is a 2.5 hour solid phase ELISA designed to measure Angiogenin in cell culture supernates, serum, and plasma. It contains recombinant human Angiogenin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate both natural and recombinant human Angiogenin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean356717173966419113671
Standard Deviation10245047166309
CV%2.83.32.97.18.78.4

Recovery

The recovery of Angiogenin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 97-117
Citrate Plasma (n=10) 102 84-126
EDTA Plasma (n=10) 101 79-121
Heparin Plasma (n=10) 102 80-137
Serum (n=10) 103 84-126
Linearity
The following samples were diluted with Calibrator Diluent and assayed to assess linearity of the assay.
Human Angiogenin Quantikine ELISA Kit
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8° C. Do not use past expiration date.
Background: Angiogenin
Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.
    • Entrez Gene IDs
      283 (Human);
    • Alternate Names
      ANG; RNase 5; ALS9; angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4;
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 15
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    Sample Type
    1. Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer.
      Authors: Landt S, Mordelt K, Schwidde I, Barinoff J, Korlach S, Stoblen F, Lichtenegger W, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2651-5.
      Species: Human
      Sample Type: Serum
    2. Glycemic control influences serum angiogenin concentrations in patients with type 2 diabetes.
      Authors: Siebert J, Reiwer-Gostomska M, Mysliwska J
      Diabetes Care, 2010;33(8):1829-30.
      Species: Human
      Sample Type: Serum
    3. A comparative study of two angiogenic factors: vascular endothelial growth factor and angiogenin in induced sputum from asthmatic children in acute attack.
      Authors: Abdel-Rahman AM, el-Sahrigy SA, Bakr SI
      Chest, 2006;129(2):266-71.
      Species: Human
      Sample Type: Saliva
    4. Plasma angiogenin levels in acute coronary syndromes: implications for prognosis.
      Authors: Tello-Montoliu A, Marin F, Patel J, Roldan V, Mainar L, Vicente V, Sogorb F, Lip GY
      Eur. Heart J., 2007;28(24):3006-11.
      Species: Human
      Sample Type: Plasma
    5. Low serum angiogenin concentrations in patients with type 2 diabetes.
      Authors: Siebert J, Reiwer-Gostomska M, Babinska Z, Mysliwska J, Mysliwski A, Skopinska-Rozewska E, Sommer E, Skopinski P
      Diabetes Care, 2007;30(12):3086-7.
      Species: Human
      Sample Type: Serum
    6. Comparison of cytokeratin 20 RNA and angiogenin in voided urine samples as diagnostic tools for bladder carcinoma.
      Authors: Eissa S, Kenawy G, Swellam M, El-Fadle AA, Abd El-Aal AA, El-Ahmady O
      Clin. Biochem., 2004;37(9):803-10.
      Species: Human
      Sample Type: Urine
    7. Elevated angiogenin levels in the peritoneal fluid of women with endometriosis correlate with the extent of the disorder.
      Authors: Suzumori N, Zhao XX, Suzumori K
      Fertil. Steril., 2004;82(1):93-6.
      Species: Human
      Sample Type: Peritoneal Fluid
    8. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
      Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
      Exp. Hematol., 2007;35(7):1119-31.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
      Authors: Bourlev V, Iljasova N, Adamyan L, Larsson A, Olovsson M
      Fertil. Steril., 2010;94(1):52-7.
      Species: Human
      Sample Type: Serum
    10. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
      Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
      Br. J. Haematol., 2007;137(6):560-8.
      Species: Human
      Sample Type: Serum
    11. Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
      Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
      Leuk. Lymphoma, 2006;47(6):1034-40.
      Species: Human
      Sample Type: Serum
    12. Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor κB and miR-221.
      Authors: Santhekadur PK, Das SK, Gredler R, Chen D, Srivastava J, Robertson C, Baldwin AS, Fisher PB, Sarkar D
      J. Biol. Chem., 2012;287(17):13952-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
      Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
      Diabetologia, 2011;54(9):2358-67.
      Species: Human
      Sample Type: Serum
    14. Plasma haemoxygenase-1 in coronary artery disease. A comparison with angiogenin, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 and vascular endothelial growth factor.
      Authors: Idriss NK, Lip GY, Balakrishnan B
      Thromb. Haemost., 2010;104(5):1029-37.
      Species: Human
      Sample Type: Plasma
    15. Arterial Hypertension Is Characterized by Imbalance of Pro-Angiogenic versus Anti-Angiogenic Factors.
      Authors: Marek-Trzonkowska N, Kwieczynska A, Reiwer-Gostomska M, Kolinski T, Molisz A, Siebert J
      PLoS ONE, 2015;10(5):e0126190.
      Species: Human
      Sample Type: Serum
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