The Quantikine Human Angiogenin Immunoassay is a 2.5 hour solid phase ELISA designed to measure Angiogenin in cell culture supernates, serum, and plasma. It contains recombinant human Angiogenin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate both natural and recombinant human Angiogenin.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of Angiogenin spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Media (n=4)
Citrate Plasma (n=10)
EDTA Plasma (n=10)
Heparin Plasma (n=10)
The following samples were diluted with Calibrator Diluent and assayed to assess linearity of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
50 µL Assay Diluent
Add 50 µL of Assay Diluent to each well.
200 µL Standard, Control, or Sample
Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
Aspirate each well and wash, repeating the process twice for a total of 3 washes.
200 µL Conjugate
Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
Aspirate and wash 3 times.
200 µL Substrate Solution
Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution
Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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