Human Angiogenin Quantikine ELISA Kit

  (17 citations)     
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Product Details
Assay Procedure
Citations (17)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    2.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
  • Sensitivity
    6 pg/mL
  • Assay Range
    78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human Angiogenin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC21 , Quantikine Immunoassay Control Group 4 - Please Inquire
Product Summary
The Quantikine Human Angiogenin Immunoassay is a 2.5 hour solid phase ELISA designed to measure Angiogenin in cell culture supernates, serum, and plasma. It contains recombinant human Angiogenin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate both natural and recombinant human Angiogenin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 356 717 1739 664 1911 3671
Standard Deviation 10 24 50 47 166 309
CV% 2.8 3.3 2.9 7.1 8.7 8.4

Recovery

The recovery of Angiogenin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 97-117
Citrate Plasma (n=10) 102 84-126
EDTA Plasma (n=10) 101 79-121
Heparin Plasma (n=10) 102 80-137
Serum (n=10) 103 84-126
Linearity
The following samples were diluted with Calibrator Diluent and assayed to assess linearity of the assay.
 Angiogenin [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiogenin
Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.
  • Entrez Gene IDs:
    283 (Human)
  • Alternate Names:
    ALS9; ANG; Angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Sample Type
  1. Real-Time and Non-invasive Monitoring of the Activation of the IRE1?-XBP1 Pathway in Individuals with Hemodynamic Impairment
    Authors: B Fohlen, Q Tavernier, TM Huynh, C Caradeuc, D Le Corre, G Bertho, B Cholley, N Pallet
    EBioMedicine, 2017;0(0):.
    Species: Human
    Sample Type: Urine
  2. Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
    Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
    Oncotarget, 2017;8(37):62081-62098.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Arterial Hypertension Is Characterized by Imbalance of Pro-Angiogenic versus Anti-Angiogenic Factors.
    Authors: Marek-Trzonkowska N, Kwieczynska A, Reiwer-Gostomska M, Kolinski T, Molisz A, Siebert J
    PLoS ONE, 2015;10(5):e0126190.
    Species: Human
    Sample Type: Serum
  4. Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor κB and miR-221.
    Authors: Santhekadur PK, Das SK, Gredler R, Chen D, Srivastava J, Robertson C, Baldwin AS, Fisher PB, Sarkar D
    J. Biol. Chem., 2012;287(17):13952-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
    Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
    Diabetologia, 2011;54(9):2358-67.
    Species: Human
    Sample Type: Serum
  6. Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer.
    Authors: Landt S, Mordelt K, Schwidde I, Barinoff J, Korlach S, Stoblen F, Lichtenegger W, Sehouli J, Kummel S
    Anticancer Res., 2011;31(8):2651-5.
    Species: Human
    Sample Type: Serum
  7. Glycemic control influences serum angiogenin concentrations in patients with type 2 diabetes.
    Authors: Siebert J, Reiwer-Gostomska M, Mysliwska J
    Diabetes Care, 2010;33(8):1829-30.
    Species: Human
    Sample Type: Serum
  8. Plasma haemoxygenase-1 in coronary artery disease. A comparison with angiogenin, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 and vascular endothelial growth factor.
    Authors: Idriss NK, Lip GY, Balakrishnan B
    Thromb. Haemost., 2010;104(5):1029-37.
    Species: Human
    Sample Type: Plasma
  9. Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
    Authors: Bourlev V, Iljasova N, Adamyan L, Larsson A, Olovsson M
    Fertil. Steril., 2010;94(1):52-7.
    Species: Human
    Sample Type: Serum
  10. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
    Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
    Exp. Hematol., 2007;35(7):1119-31.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
    Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
    Br. J. Haematol., 2007;137(6):560-8.
    Species: Human
    Sample Type: Serum
  12. Low serum angiogenin concentrations in patients with type 2 diabetes.
    Authors: Siebert J, Reiwer-Gostomska M, Babinska Z, Mysliwska J, Mysliwski A, Skopinska-Rozewska E, Sommer E, Skopinski P
    Diabetes Care, 2007;30(12):3086-7.
    Species: Human
    Sample Type: Serum
  13. Plasma angiogenin levels in acute coronary syndromes: implications for prognosis.
    Authors: Tello-Montoliu A, Marin F, Patel J, Roldan V, Mainar L, Vicente V, Sogorb F, Lip GY
    Eur. Heart J., 2007;28(24):3006-11.
    Species: Human
    Sample Type: Plasma
  14. Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
    Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
    Leuk. Lymphoma, 2006;47(6):1034-40.
    Species: Human
    Sample Type: Serum
  15. A comparative study of two angiogenic factors: vascular endothelial growth factor and angiogenin in induced sputum from asthmatic children in acute attack.
    Authors: Abdel-Rahman AM, el-Sahrigy SA, Bakr SI
    Chest, 2006;129(2):266-71.
    Species: Human
    Sample Type: Saliva
  16. Elevated angiogenin levels in the peritoneal fluid of women with endometriosis correlate with the extent of the disorder.
    Authors: Suzumori N, Zhao XX, Suzumori K
    Fertil. Steril., 2004;82(1):93-6.
    Species: Human
    Sample Type: Peritoneal Fluid
  17. Comparison of cytokeratin 20 RNA and angiogenin in voided urine samples as diagnostic tools for bladder carcinoma.
    Authors: Eissa S, Kenawy G, Swellam M, El-Fadle AA, Abd El-Aal AA, El-Ahmady O
    Clin. Biochem., 2004;37(9):803-10.
    Species: Human
    Sample Type: Urine
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