Human Angiopoietin-2 Quantikine ELISA Kit

(47 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL)
  • Sensitivity
    21.3 pg/mL
  • Assay Range
    46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human Angiopoietin-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Angiopoietin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-2 in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human Angiopoietin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-2.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Standard Deviation16.445.654.428.873111


The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-113
EDTA Plasma (n=4) 95 85-104
Heparin Plasma (n=4) 94 87-104
Serum (n=4) 100 90-107
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Angiopoietin-2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-2
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
    • Entrez Gene IDs
      285 (Human); 11601 (Mouse);
    • Alternate Names
      AGPT2; ANG2; ANG-2; angiopoietin 2; angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 47
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    Sample Type
    1. Mechanism of angiopoietin-1 upregulation in Kaposi's sarcoma-associated herpesvirus-infected PEL cell lines.
      Authors: Zheng X, Ohsaki E, Ueda K
      J Virol, 2015;89(9):4786-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
      Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
      PLoS ONE, 2015;10(8):e0136205.
      Species: Human
      Sample Type: Plasma
    3. Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ratio as indicators of potential severity of Plasmodium vivax malaria in patients with thrombocytopenia.
      Authors: Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C
      PLoS ONE, 2014;9(10):e109246.
      Species: Human
      Sample Type: Serum
    4. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
      Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
      J Virol, 2014;88(1):483-9.
    5. Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases.
      Authors: Wertenbroek, Marieke, Schepers, Marianne, Kamminga-Rasker, Hannetta, Bottema, Jan T, Muller Kobold, Anneke C, Roelofsen, Han, de Jong, Koert P
      BMC Cancer, 2013;13(1):266.
      Species: Human
      Sample Type: Serum
    6. Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
      Proc Natl Acad Sci U S A, 2012;109(25):10024-9.
      Species: Primate - Papio cynocephalus (Yellow Baboon)
      Sample Type: Serum
    7. Intermediate monocytes but not TIE2-expressing monocytes are a sensitive diagnostic indicator for colorectal cancer.
      Authors: Schauer D, Starlinger P, Reiter C, Jahn N, Zajc P, Buchberger E, Bachleitner-Hofmann T, Bergmann M, Stift A, Gruenberger T, Brostjan C
      PLoS ONE, 2012;7(9):e44450.
      Species: Human
      Sample Type: Plasma
    8. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
      Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
      Clin. Cancer Res., 2012;18(8):2290-300.
      Species: Human
      Sample Type: Plasma
    9. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
      Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
      Cardiovasc. Res., 2011;89(1):157-65.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
      Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
      Br. J. Cancer, 2011;104(1):51-9.
      Species: Human
      Sample Type: Serum
    11. Circulating angiopoietin-2 and soluble Tie-2 in type 2 diabetes mellitus: a cross-sectional study.
      Authors: Rasul S, Reiter MH, Ilhan A, Lampichler K, Wagner L, Kautzky-Willer A
      Cardiovasc Diabetol, 2011;10(0):55.
      Species: Human
      Sample Type: Plasma
    12. Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
      Authors: Bourlev V, Iljasova N, Adamyan L, Larsson A, Olovsson M
      Fertil. Steril., 2010;94(1):52-7.
      Species: Human
      Sample Type: Serum
    13. Angiopoietin-2 plasma dosage predicts time to first treatment and overall survival in chronic lymphocytic leukemia.
      Authors: Maffei R, Martinelli S, Santachiara R
      Blood, 2010;116(4):584-92.
      Species: Human
      Sample Type: Plasma
    14. Effect of prostaglandin F2 alpha on local luteotropic and angiogenic factors during induced functional luteolysis in the bovine corpus luteum.
      Authors: Berisha B, Meyer HH, Schams D
      Biol. Reprod., 2010;82(5):940-7.
      Species: Bovine
      Sample Type: Tissue Homogenates
    15. Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
      Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
      Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(6):L849-56.
      Species: Human
      Sample Type: Tissue Homogenates
    16. Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
      Authors: Hurliman AK, Speroff L, Stouffer RL, Patton PE, Lee A, Molskness TA
      Fertil. Steril., 2010;93(5):1493-9.
      Species: Human
      Sample Type: Serum
    17. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
      Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
      Exp. Hematol., 2009;37(6):673-8.
      Species: Human
      Sample Type: Plasma
    18. Colorectal resection is associated with persistent proangiogenic plasma protein changes: postoperative plasma stimulates in vitro endothelial cell growth, migration, and invasion.
      Authors: Shantha Kumara HM, Feingold D, Kalady M, Dujovny N, Senagore A, Hyman N, Cekic V, Whelan RL
      Ann. Surg., 2009;249(6):973-7.
      Species: Human
      Sample Type: Plasma
    19. Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma.
      Authors: Helfrich I, Edler L, Sucker A, Thomas M, Christian S, Schadendorf D, Augustin HG
      Clin. Cancer Res., 2009;15(4):1384-92.
      Species: Human
      Sample Type: Serum
    20. Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
      Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
      Ann. Hematol., 2009;88(7):667-71.
      Species: Human
      Sample Type: Plasma
    21. Structural stability of neoangiogenic intramyocardial microvessels supports functional recovery in chronic ischemic myocardium.
      Authors: Shim WS, Lim SY, Li SQ, Gu Y, Ong HC, Song IC, Chuah SC, Wong P
      J. Mol. Cell. Cardiol., 2008;45(1):70-80.
      Species: Porcine
      Sample Type: Tissue Homogenates
    22. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
      Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
      Exp. Hematol., 2008;36(8):1035-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. Plasma levels of soluble Tie2 and vascular endothelial growth factor distinguish critical limb ischemia from intermittent claudication in patients with peripheral arterial disease.
      Authors: Findley CM, Mitchell RG, Duscha BD, Annex BH, Kontos CD
      J. Am. Coll. Cardiol., 2008;52(5):387-93.
      Species: Human
      Sample Type: Plasma
    24. Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
      Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
      Ann. Surg., 2008;247(2):320-6.
      Species: Human
      Sample Type: Plasma
    25. Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria.
      Authors: Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, Piera K, Price RN, Duffull SB, Celermajer DS, Anstey NM
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(44):17097-102.
      Species: Human
      Sample Type: Plasma
    26. Relationship of soluble CD40 ligand to vascular endothelial growth factor, angiopoietins, and tissue factor in atrial fibrillation: a link among platelet activation, angiogenesis, and thrombosis?
      Authors: Choudhury A, Freestone B, Patel J, Lip GY
      Chest, 2007;132(6):1913-9.
      Species: Human
      Sample Type: Plasma
    27. Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
      Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
      Cancer Lett., 2007;248(1):131-6.
      Species: Human
      Sample Type: Plasma
    28. Serum angiopoietin-2 as a clinical marker for lung cancer.
      Authors: Park JH, Park KJ, Kim YS, Sheen SS, Lee KS, Lee HN, Oh YJ, Hwang SC
      Chest, 2007;132(1):200-6.
      Species: Human
      Sample Type: Serum
    29. Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
      Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
      Br. J. Cancer, 2007;97(7):877-82.
      Species: Human
      Sample Type: Plasma
    30. Angiopoietin-2 as a contributing factor of exercise-induced bronchoconstriction in asthmatic patients receiving inhaled corticosteroid therapy.
      Authors: Kanazawa H, Tochino Y, Asai K
      J. Allergy Clin. Immunol., 2007;121(2):390-5.
      Species: Human
      Sample Type: Sputum
    31. Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
      Authors: Caine GJ, Ryan P, Lip GY, Blann AD
      Cancer Lett., 2007;251(2):296-301.
      Species: Human
      Sample Type: Plasma
    32. Roles of angiopoietin-1 and angiopoietin-2 on airway microvascular permeability in asthmatic patients.
      Authors: Kanazawa H, Nomura S, Asai K
      Chest, 2007;131(4):1035-41.
      Species: Human
      Sample Type: Sputum
    33. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
      Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
      Br. J. Haematol., 2007;137(6):560-8.
      Species: Human
      Sample Type: Serum
    34. Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells.
      Authors: Vart RJ, Nikitenko LL, Lagos D, Trotter MW, Cannon M, Bourboulia D, Gratrix F, Takeuchi Y, Boshoff C
      Cancer Res., 2007;67(9):4042-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    35. Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
      Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
      Cytokine, 2006;36(5):291-5.
      Species: Human
      Sample Type: Serum
    36. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
      Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
      Br. J. Cancer, 2006;94(4):524-31.
      Species: Human
      Sample Type: Serum
    37. Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures.
      Authors: Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ
      Arthritis Rheum., 2006;54(10):3152-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    38. Differential levels of soluble angiopoietin-2 and Tie-2 in patients with haematological malignancies.
      Authors: Quartarone E, Alonci A, Allegra A, Bellomo G, Calabro L, D'Angelo A, Del Fabro V, Grasso A, Cincotta M, Musolino C
      Eur. J. Haematol., 2006;77(6):480-5.
      Species: Human
      Sample Type: Serum
    39. Potential role of soluble angiopoietin-2 and Tie-2 in patients with inflammatory bowel disease.
      Authors: Koutroubakis IE, Xidakis C, Karmiris K, Sfiridaki A, Kandidaki E, Kouroumalis EA
      Eur. J. Clin. Invest., 2006;36(2):127-32.
      Species: Human
      Sample Type: Serum
    40. Circulating levels of total angiopoietin-2 and the soluble Tie-2 receptor in women during ovarian stimulation and early gestation.
      Authors: Molskness TA, Stouffer RL, Burry KA, Gorrill MJ, Lee DM, Patton PE
      Fertil. Steril., 2006;86(5):1531-3.
      Species: Human
      Sample Type: Serum
    41. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death.
      Authors: Bhandari V, Choo-Wing R, Lee CG, Zhu Z, Nedrelow JH, Chupp GL, Zhang X, Matthay MA, Ware LB, Homer RJ, Lee PJ, Geick A, de Fougerolles AR, Elias JA
      Nat. Med., 2006;12(11):1286-93.
      Species: Human
      Sample Type: Plasma
    42. Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
      Authors: Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM
      Am. J. Obstet. Gynecol., 2006;194(2):564-71.
      Species: Human
      Sample Type: Tissue Homogenates
    43. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    44. Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy.
      Authors: Watanabe D, Suzuma K, Suzuma I, Ohashi H, Ojima T, Takagi H
      Am. J. Ophthalmol., 2005;139(3):476-81.
      Species: Human
      Sample Type: Vitreous Humor
    45. Bi-directional cell contact-dependent regulation of gene expression between endothelial cells and osteoblasts in a three-dimensional spheroidal coculture model.
      Authors: Stahl A, Wenger A, Weber H, Stark GB, Augustin HG, Finkenzeller G
      Biochem. Biophys. Res. Commun., 2004;322(2):684-92.
      Species: Human
      Sample Type: Cell Culture Supernates
    46. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
      Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
      Crit. Care Med., 0;0(0):.
      Species: Human
      Sample Type: Plasma
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    ELISA Controls
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    Quantikine Immunoassay Control Set 832 Human Angiopoietin-2

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