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Human Angiopoietin-2 Quantikine ELISA Kit

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QC43: Quantikine Immunoassay Control Set 832 Human Angiopoietin-2
Human Angiopoietin-2 ELISA Standard Curve
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Citations (76)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Angiopoietin-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL)
Sensitivity
21.3 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human Angiopoietin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Angiopoietin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-2 in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human Angiopoietin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 237 703 1301 276 805 1494
Standard Deviation 16.4 45.6 54.4 28.8 73 111
CV% 6.9 6.5 4.2 10.4 9.1 7.4

Recovery

The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-113
EDTA Plasma (n=4) 95 85-104
Heparin Plasma (n=4) 94 87-104
Serum (n=4) 100 90-107

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Angiopoietin-2 ELISA Linearity

Data Examples

Human Angiopoietin-2 ELISA Standard Curve View Larger

Human Angiopoietin-2 ELISA Standard Curve

Product Datasheets

Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Angiopoietin-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

76 Citations: Showing 1 - 10
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  1. Adhesion of T cells to endothelial cells facilitates blinatumomab-associated neurologic adverse events
    Authors: M Klinger, G Zugmaier, V Nägele, ME Goebeler, C Brandl, M Stelljes, H Lassmann, A von Stacke, RC Bargou, P Kufer
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Plasma
  2. Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p
    Authors: C Gao, Z Gong, D Wang, J Huang, Y Qian, M Nie, W Jiang, X Liu, H Luo, J Yuan, T Xiang, S An, W Quan, H Wei, J Zhang, R Jiang
    Aging (Albany NY), 2019;11(24):12147-12164.
  3. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk,
    Kidney Int Rep, 2019;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  4. Relationship of mottling score, skin microcirculatory perfusion indices and biomarkers of endothelial dysfunction in patients with septic shock: an observational study
    Authors: S Kazune, A Caica, K Volceka, O Suba, U Rubins, A Grabovskis
    Crit Care, 2019;23(1):311.
    Species: Human
    Sample Types: Plasma
  5. Microcirculatory perfusion disturbances following cardiac surgery with cardiopulmonary bypass are associated with in vitro endothelial hyperpermeability and increased angiopoietin-2 levels
    Authors: NAM Dekker, ALI van Leeuwe, WWJ van Strien, J Majolée, R Szulcek, ABA Vonk, PL Hordijk, C Boer, CE van den Br
    Crit Care, 2019;23(1):117.
    Species: Human
    Sample Types: Plasma
  6. Fibroblast growth factor 23, endothelium biomarkers and acute kidney injury in critically-ill patients
    Authors: FM de Oliveir, CB Araújo, DF de Freitas, BFT Arruda, LJM de Macêdo, VB Salles, GC Meneses, AMC Martins, AB Libório
    J Transl Med, 2019;17(1):121.
    Species: Human
    Sample Types: Plasma
  7. Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
    Authors: M Varas-Godo, S Acuña-Gall, S Venegas-Du, C Hill, A Caceres-Ve, O Realini, LJ Monteiro, G Zavala, M Khoury, R Romero, G Rice, SE Illanes
    Stem Cells Int, 2019;2019(0):1916542.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis
    Authors: C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein
    Peptides, 2018;102(0):31-37.
    Species: Human
    Sample Types: Plasma
  9. Different patterns of inflammatory and angiogenic factors are associated with peritoneal small solute transport and peritoneal protein clearance in peritoneal dialysis patients
    Authors: Y Shi, H Yan, J Yuan, H Zhang, J Huang, Z Ni, J Qian, W Fang
    BMC Nephrol, 2018;19(1):119.
    Species: Human
    Sample Types: Serum
  10. Does the Automatic Measurement of Interleukin 6 Allow for Prediction of Complications during the First 48 h of Acute Pancreatitis?
    Authors: W Kolber, P Dumnicka, M Maraj, B Ku?nierz-C, P Ceranowicz, M P?dziwiatr, B Maziarz, M Mazur-Lask, M Ku?niewski, M Sporek, J Walocha
    Int J Mol Sci, 2018;19(6):.
    Species: Human
    Sample Types: Serum
  11. Profiling of selected angiogenesis-related genes in serous ovarian cancer patients
    Authors: P Ku?, R Charkiewic, D Klasa-Mazu, T Milczek, B Mroczko, J Nikli?ski, P Lauda?ski
    Adv Med Sci, 2017;62(1):116-120.
    Species: Human
    Sample Types: Cell Lysates
  12. Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D
    Authors: S Demolli, A Doddaballa, K Devraj, K Stark, Y Manavski, A Eckart, CM Zehendner, T Lucas, T Korff, M Hecker, S Massberg, S Liebner, D Kaluza, RA Boon, S Dimmeler
    Cardiovasc. Res., 2017;113(6):681-691.
    Species: Human
    Sample Types: Cell Lysates
  13. Tie2 as a novel key factor of microangiopathy in systemic sclerosis
    Authors: F Moritz, J Schniering, JHW Distler, RE Gay, S Gay, O Distler, B Maurer
    Arthritis Res. Ther., 2017;19(1):105.
    Species: Human
    Sample Types: Serum
  14. Myeloperoxidase-derived 2-chlorofatty acids contribute to human sepsis mortality via acute respiratory distress syndrome
    Authors: NJ Meyer, JP Reilly, R Feng, JD Christie, SL Hazen, CJ Albert, JD Franke, CL Hartman, J McHowat, DA Ford
    JCI Insight, 2017;2(23):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Angiopoietin-2 and soluble Tie-2 receptor plasma levels in children with obstructive sleep apnea and obesity
    Authors: D Gozal, A Khalyfa, Z Qiao, DL Smith, MF Philby, D Koren, L Kheirandis
    Obesity (Silver Spring), 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  16. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
    Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
    Lab. Invest., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Serum Concentrations of Angiopoietin-2 and Soluble fms-Like Tyrosine Kinase 1 (sFlt-1) Are Associated with Coagulopathy among Patients with Acute Pancreatitis
    Authors: P Dumnicka, B Ku?nierz-C, M Sporek, M Mazur-Lask, K Gil, M Ku?niewski, P Ceranowicz, Z Warzecha, A Dembi?ski, J Bonior, R Dro?d?
    Int J Mol Sci, 2017;18(4):.
    Species: Human
    Sample Types: Serum
  18. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Angiopoietin-2, Angiopoietin-1 and subclinical cardiovascular disease in Chronic Kidney Disease
    Sci Rep, 2016;6(0):39400.
    Species: Human
    Sample Types: Serum
  20. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
    Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
    J Am Heart Assoc, 2016;5(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. IL-35 Inhibits Angiogenesis through VEGF/Ang2/Tie2 Pathway in Rheumatoid Arthritis
    Authors: Shenyi Jiang
    Cell. Physiol. Biochem, 2016;40(5):1105-1116.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
    Sci Adv, 2016;2(4):e1501290.
    Species: Human
    Sample Types: Cell Lysates
  23. Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
    Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
    PLoS ONE, 2015;10(8):e0136205.
    Species: Human
    Sample Types: Plasma
  24. Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial.
    Authors: Verstraete M, Debucquoy A, Dekervel J, Van Pelt J, Verslype C, Devos E, Chiritescu G, Dumon K, D'Hoore A, Gevaert O, Sagaert X, Van Cutsem E, Haustermans K
    Br J Cancer, 2015;112(8):1314-25.
    Species: Human
    Sample Types: Whole Blood
  25. Kidney function, endothelial activation and atherosclerosis in black and white Africans with rheumatoid arthritis.
    Authors: Dessein, Patrick, Hsu, Hon-Chun, Tsang, Linda, Millen, Aletta M, Woodiwiss, Angela J, Norton, Gavin R, Solomon, Ahmed, Gonzalez-Gay, Miguel A
    PLoS ONE, 2015;10(3):e0121693.
    Species: Human
    Sample Types: Plasma
  26. Mechanism of angiopoietin-1 upregulation in Kaposi's sarcoma-associated herpesvirus-infected PEL cell lines.
    Authors: Zheng X, Ohsaki E, Ueda K
    J Virol, 2015;89(9):4786-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. A randomized phase II study of capecitabine-based chemoradiation with or without bevacizumab in resectable locally advanced rectal cancer: clinical and biological features.
    Authors: Salazar R, Capdevila J, Laquente B, Manzano J, Pericay C, Villacampa M, Lopez C, Losa F, Safont M, Gomez A, Alonso V, Escudero P, Gallego J, Sastre J, Gravalos C, Biondo S, Palacios A, Aranda E
    BMC Cancer, 2015;15(0):60.
    Species: Human
    Sample Types: Plasma
  28. Association of angiopoietin-2 with renal outcome in chronic kidney disease.
    Authors: Tsai, Yi-Chun, Chiu, Yi-Wen, Tsai, Jer-Chia, Kuo, Hung-Tie, Lee, Su-Chu, Hung, Chi-Chih, Lin, Ming-Yen, Hwang, Shang-Jy, Kuo, Mei-Chua, Chen, Hung-Chu
    PLoS ONE, 2014;9(10):e108862.
    Species: Human
    Sample Types: Plasma
  29. Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ratio as indicators of potential severity of Plasmodium vivax malaria in patients with thrombocytopenia.
    Authors: Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C
    PLoS ONE, 2014;9(10):e109246.
    Species: Human
    Sample Types: Serum
  30. Angiopoietin-2 and biliary diseases: elevated serum, but not bile levels are associated with cholangiocarcinoma.
    Authors: Voigtlander T, David S, Thamm K, Schlue J, Metzger J, Manns M, Lankisch T
    PLoS ONE, 2014;9(5):e97046.
    Species: Human
    Sample Types: Bile
  31. Serum angiopoietin-2 and soluble VEGFR-2 levels predict malignancy of ovarian neoplasm and poor prognosis in epithelial ovarian cancer.
    Authors: Sallinen H, Heikura T, Koponen J, Kosma V, Heinonen S, Yla-Herttuala S, Anttila M
    BMC Cancer, 2014;14(0):696.
    Species: Human
    Sample Types: Serum
  32. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
    Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
    J Virol, 2014;88(1):483-9.
  33. Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases.
    Authors: Wertenbroek, Marieke, Schepers, Marianne, Kamminga-Rasker, Hannetta, Bottema, Jan T, Muller Kobold, Anneke C, Roelofsen, Han, de Jong, Koert P
    BMC Cancer, 2013;13(1):266.
    Species: Human
    Sample Types: Serum
  34. Intermediate monocytes but not TIE2-expressing monocytes are a sensitive diagnostic indicator for colorectal cancer.
    Authors: Schauer D, Starlinger P, Reiter C, Jahn N, Zajc P, Buchberger E, Bachleitner-Hofmann T, Bergmann M, Stift A, Gruenberger T, Brostjan C
    PLoS ONE, 2012;7(9):e44450.
    Species: Human
    Sample Types: Plasma
  35. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
    Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
    Clin. Cancer Res., 2012;18(8):2290-300.
    Species: Human
    Sample Types: Plasma
  36. Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
    Proc Natl Acad Sci U S A, 2012;109(25):10024-9.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Serum
  37. Circulating angiopoietin-2 and soluble Tie-2 in type 2 diabetes mellitus: a cross-sectional study.
    Authors: Rasul S, Reiter MH, Ilhan A, Lampichler K, Wagner L, Kautzky-Willer A
    Cardiovasc Diabetol, 2011;10(0):55.
    Species: Human
    Sample Types: Plasma
  38. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
    Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
    Br. J. Cancer, 2011;104(1):51-9.
    Species: Human
    Sample Types: Serum
  39. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2011;89(1):157-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Effect of prostaglandin F2 alpha on local luteotropic and angiogenic factors during induced functional luteolysis in the bovine corpus luteum.
    Authors: Berisha B, Meyer HH, Schams D
    Biol. Reprod., 2010;82(5):940-7.
    Species: Bovine
    Sample Types: Tissue Homogenates
  41. Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
    Authors: Bourlev V, Iljasova N, Adamyan L, Larsson A, Olovsson M
    Fertil. Steril., 2010;94(1):52-7.
    Species: Human
    Sample Types: Serum
  42. Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
    Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(6):L849-56.
    Species: Human
    Sample Types: Tissue Homogenates
  43. Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
    Authors: Hurliman AK, Speroff L, Stouffer RL, Patton PE, Lee A, Molskness TA
    Fertil. Steril., 2010;93(5):1493-9.
    Species: Human
    Sample Types: Serum
  44. Angiopoietin-2 plasma dosage predicts time to first treatment and overall survival in chronic lymphocytic leukemia.
    Authors: Maffei R, Martinelli S, Santachiara R
    Blood, 2010;116(4):584-92.
    Species: Human
    Sample Types: Plasma
  45. Colorectal resection is associated with persistent proangiogenic plasma protein changes: postoperative plasma stimulates in vitro endothelial cell growth, migration, and invasion.
    Authors: Shantha Kumara HM, Feingold D, Kalady M, Dujovny N, Senagore A, Hyman N, Cekic V, Whelan RL
    Ann. Surg., 2009;249(6):973-7.
    Species: Human
    Sample Types: Plasma
  46. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
    Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
    Exp. Hematol., 2009;37(6):673-8.
    Species: Human
    Sample Types: Plasma
  47. Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
    Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
    Ann. Hematol., 2009;88(7):667-71.
    Species: Human
    Sample Types: Plasma
  48. Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma.
    Authors: Helfrich I, Edler L, Sucker A, Thomas M, Christian S, Schadendorf D, Augustin HG
    Clin. Cancer Res., 2009;15(4):1384-92.
    Species: Human
    Sample Types: Serum
  49. Structural stability of neoangiogenic intramyocardial microvessels supports functional recovery in chronic ischemic myocardium.
    Authors: Shim WS, Lim SY, Li SQ, Gu Y, Ong HC, Song IC, Chuah SC, Wong P
    J. Mol. Cell. Cardiol., 2008;45(1):70-80.
    Species: Porcine
    Sample Types: Tissue Homogenates
  50. Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
    Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
    Ann. Surg., 2008;247(2):320-6.
    Species: Human
    Sample Types: Plasma
  51. Plasma levels of soluble Tie2 and vascular endothelial growth factor distinguish critical limb ischemia from intermittent claudication in patients with peripheral arterial disease.
    Authors: Findley CM, Mitchell RG, Duscha BD, Annex BH, Kontos CD
    J. Am. Coll. Cardiol., 2008;52(5):387-93.
    Species: Human
    Sample Types: Plasma
  52. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria.
    Authors: Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, Piera K, Price RN, Duffull SB, Celermajer DS, Anstey NM
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(44):17097-102.
    Species: Human
    Sample Types: Plasma
  54. Roles of angiopoietin-1 and angiopoietin-2 on airway microvascular permeability in asthmatic patients.
    Authors: Kanazawa H, Nomura S, Asai K
    Chest, 2007;131(4):1035-41.
    Species: Human
    Sample Types: Sputum
  55. Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
    Authors: Caine GJ, Ryan P, Lip GY, Blann AD
    Cancer Lett., 2007;251(2):296-301.
    Species: Human
    Sample Types: Plasma
  56. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
    Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
    Br. J. Haematol., 2007;137(6):560-8.
    Species: Human
    Sample Types: Serum
  57. Serum angiopoietin-2 as a clinical marker for lung cancer.
    Authors: Park JH, Park KJ, Kim YS, Sheen SS, Lee KS, Lee HN, Oh YJ, Hwang SC
    Chest, 2007;132(1):200-6.
    Species: Human
    Sample Types: Serum
  58. Angiopoietin-2 as a contributing factor of exercise-induced bronchoconstriction in asthmatic patients receiving inhaled corticosteroid therapy.
    Authors: Kanazawa H, Tochino Y, Asai K
    J. Allergy Clin. Immunol., 2007;121(2):390-5.
    Species: Human
    Sample Types: Sputum
  59. Relationship of soluble CD40 ligand to vascular endothelial growth factor, angiopoietins, and tissue factor in atrial fibrillation: a link among platelet activation, angiogenesis, and thrombosis?
    Authors: Choudhury A, Freestone B, Patel J, Lip GY
    Chest, 2007;132(6):1913-9.
    Species: Human
    Sample Types: Plasma
  60. Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
    Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
    Cancer Lett., 2007;248(1):131-6.
    Species: Human
    Sample Types: Plasma
  61. Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
    Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
    Br. J. Cancer, 2007;97(7):877-82.
    Species: Human
    Sample Types: Plasma
  62. Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells.
    Authors: Vart RJ, Nikitenko LL, Lagos D, Trotter MW, Cannon M, Bourboulia D, Gratrix F, Takeuchi Y, Boshoff C
    Cancer Res., 2007;67(9):4042-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Differential levels of soluble angiopoietin-2 and Tie-2 in patients with haematological malignancies.
    Authors: Quartarone E, Alonci A, Allegra A, Bellomo G, Calabro L, D'Angelo A, Del Fabro V, Grasso A, Cincotta M, Musolino C
    Eur. J. Haematol., 2006;77(6):480-5.
    Species: Human
    Sample Types: Serum
  64. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death.
    Authors: Bhandari V, Choo-Wing R, Lee CG, Zhu Z, Nedrelow JH, Chupp GL, Zhang X, Matthay MA, Ware LB, Homer RJ, Lee PJ, Geick A, de Fougerolles AR, Elias JA
    Nat. Med., 2006;12(11):1286-93.
    Species: Human
    Sample Types: Plasma
  65. Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
    Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
    Cytokine, 2006;36(5):291-5.
    Species: Human
    Sample Types: Serum
  66. Potential role of soluble angiopoietin-2 and Tie-2 in patients with inflammatory bowel disease.
    Authors: Koutroubakis IE, Xidakis C, Karmiris K, Sfiridaki A, Kandidaki E, Kouroumalis EA
    Eur. J. Clin. Invest., 2006;36(2):127-32.
    Species: Human
    Sample Types: Serum
  67. Circulating levels of total angiopoietin-2 and the soluble Tie-2 receptor in women during ovarian stimulation and early gestation.
    Authors: Molskness TA, Stouffer RL, Burry KA, Gorrill MJ, Lee DM, Patton PE
    Fertil. Steril., 2006;86(5):1531-3.
    Species: Human
    Sample Types: Serum
  68. Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
    Authors: Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM
    Am. J. Obstet. Gynecol., 2006;194(2):564-71.
    Species: Human
    Sample Types: Tissue Homogenates
  69. Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures.
    Authors: Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ
    Arthritis Rheum., 2006;54(10):3152-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
    Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
    Br. J. Cancer, 2006;94(4):524-31.
    Species: Human
    Sample Types: Serum
  71. Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy.
    Authors: Watanabe D, Suzuma K, Suzuma I, Ohashi H, Ojima T, Takagi H
    Am. J. Ophthalmol., 2005;139(3):476-81.
    Species: Human
    Sample Types: Vitreous Humor
  72. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Bi-directional cell contact-dependent regulation of gene expression between endothelial cells and osteoblasts in a three-dimensional spheroidal coculture model.
    Authors: Stahl A, Wenger A, Weber H, Stark GB, Augustin HG, Finkenzeller G
    Biochem. Biophys. Res. Commun., 2004;322(2):684-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
    Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
    Crit. Care Med., 0;0(0):.
    Species: Human
    Sample Types: Plasma
  75. Evaluation of plasma angiopoietin-2 and vascular endothelial growth factor in healthy dogs and dogs with systemic inflammatory response syndrome or sepsis.
    Authors: Konig M, Nentwig A, Marti E, Mirkovitch J, Adamik K, Schuller S
    J Vet Intern Med, 0;0(0):.
    Species: Canine
    Sample Types: Plasma

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ELISA Controls

Quantikine Immunoassay Control Set 832 Human Angiopoietin-2

QC43
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Supplemental ELISA Products

Quantikine ELISA Wash Buffer 1

WA126
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8 Reviews
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Human Angiopoietin-2 Quantikine ELISA Kit
By Anonymous on 05/16/2020
Application: Sample Tested: Serum
Serum Human Angiopoietin-2 Quantikine ELISA Kit DANG20
Human Angiopoietin-2 Quantikine ELISA Kit
By Anonymous on 09/14/2019
Application: Sample Tested: Recombinant protein
Human Angiopoietin-2 Quantikine ELISA Kit
By Anonymous on 05/24/2019
Application: Sample Tested: Plasma

another excellent ELISA from R and D