Key Product Details

Species Reactivity

Validated:

Human

Cited:

Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 156215
View all Angiopoietin-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-4
Met1-Ile503
Accession # Q9Y264

Specificity

Detects human Angiopoietin-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Ang‑1, rhAng‑2, rhAng-X, or rhANGPTL3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Angiopoietin-4 Antibody (156215)

Angiopoietin-4 antibody in Human Kidney by Immunohistochemistry (IHC-P).

Angiopoietin‑4 in Human Kidney.

Angiopoietin-4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human Angiopoietin-4 Monoclonal Antibody (Catalog # MAB964) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Angiopoietin-4 Antibody (156215)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1 µg/mL
Sample: Recombinant Human Angiopoietin‑4 (Catalog # 964-AN)

Reviewed Applications

Read 1 review rated 5 using MAB964 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-4

Human Angiopoietin-4 (Ang-4) (1), alternatively named Ang-3 (2), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-4 cDNA encodes a 503 amino acid (aa) precursor protein with a 23 aa signal peptide. It shares 45%, 47%, and 54% aa sequence identity with human Ang-1, human Ang-2, and mouse Ang‑3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang‑3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed multiple mouse tissues. Human Ang-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

References

  1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
  2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
  3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Alternate Names

ANGPT4

Entrez Gene IDs

51378 (Human)

Gene Symbol

ANGPT4

UniProt

Q9Y264

Additional Angiopoietin-4 Products

Product Documents for Human Angiopoietin-4 Antibody (156215)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Angiopoietin-4 Antibody (156215)

For research use only

Citations for Human Angiopoietin-4 Antibody (156215)

Customer Reviews for Human Angiopoietin-4 Antibody (156215) (1)

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  • Human Angiopoietin-4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 05/30/2022
    Human Angiopoietin-4 Antibody (156215) MAB964

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