Human Angiopoietin-4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Angiopoietin-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Ang‑1, rhAng‑2, rhAng-X, or rhANGPTL3.
  • Source
    Monoclonal Mouse IgG1 Clone # 156215
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-4
    Met1-Ile503
    Accession # Q9Y264
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Angiopoietin‑4 (Catalog # 964-AN)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Angiopoietin‑4 in Human Kidney. Angiopoietin‑4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB964) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-4

Human Angiopoietin-4 (Ang-4) (1), alternatively named Ang-3 (2), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-4 cDNA encodes a 503 amino acid (aa) precursor protein with a 23 aa signal peptide. It shares 45%, 47%, and 54% aa sequence identity with human Ang-1, human Ang-2, and mouse Ang‑3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang‑3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed multiple mouse tissues. Human Ang-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

  • References:
    1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
    2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
    3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
    4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
  • Entrez Gene IDs:
    51378 (Human)
  • Alternate Names:
    AGP4; ANG3; ANG-3; ANG-4; ANG4angiopoietin-3; angiopoietin 4; Angiopoietin-3; Angiopoietin-4; ANGPT4; dJ824F16.2 (angiopoietin 4); MGC138181; MGC138183
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