Human Angiopoietin-4 Antibody

Catalog # Availability Size / Price Qty
Angiopoietin‑4 in Human Kidney.
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Product Details
Citations (1)
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Reviews (1)

Human Angiopoietin-4 Antibody Summary

Species Reactivity
Detects human Angiopoietin-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Ang‑1, rhAng‑2, rhAng-X, or rhANGPTL3.
Monoclonal Mouse IgG1 Clone # 156215
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-4
Accession # Q9Y264
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Angiopoietin‑4 (Catalog # 964-AN)
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Angiopoietin-4 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Angiopoietin‑4 in Human Kidney. Angiopoietin-4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human Angiopoietin-4 Monoclonal Antibody (Catalog # MAB964) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-4

Human Angiopoietin-4 (Ang-4) (1), alternatively named Ang-3 (2), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-4 cDNA encodes a 503 amino acid (aa) precursor protein with a 23 aa signal peptide. It shares 45%, 47%, and 54% aa sequence identity with human Ang-1, human Ang-2, and mouse Ang‑3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang‑3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed multiple mouse tissues. Human Ang-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

  1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
  2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
  3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
Entrez Gene IDs
51378 (Human)
Alternate Names
AGP4; ANG3; ANG-3; ANG-4; ANG4angiopoietin-3; angiopoietin 4; Angiopoietin-3; Angiopoietin-4; ANGPT4; dJ824F16.2 (angiopoietin 4); MGC138181; MGC138183

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Citation for Human Angiopoietin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
    Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
    Oncogene, 2022;0(0):.
    Species: Xenograft
    Sample Types: Tissue Homogenates
    Applications: Capillary Immunoblot


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Human Angiopoietin-4 Antibody
By Anonymous on 05/30/2022
Application: IHC Sample Tested: Kidney tissue Species: Human