Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all APBA3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human APBA3
Met1-Leu138
Accession # O96018

Specificity

Detects human APBA3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) X11‑ alpha and rhX11-beta is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human APBA3 Antibody

Detection of Human APBA3 antibody by Western Blot.

Detection of Human APBA3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human APBA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5689) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for APBA3 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human APBA3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APBA3

X11-gamma (Adaptor protein X11 gamma; also APBA3, Mint3 and X11L2) is a member of the X11 family of adaptor proteins. Unlike X11 alpha and -beta which are generally neuronal proteins, X11-gamma is widely expressed in all tissues. X11-gamma has many functions, including MT-MMP and Furin intracellular trafficking and APP binding. Human X11-gamma is 575 amino acids (aa) in length. It contains a poly-Ser segment (aa 165‑171), a PID region (aa 217‑381) that binds beta -amyloid precursor, and two PDZ domains (aa 393‑478 and 485‑560) that bind Arf GTPases. There is one potential splice variant that shows a 34 aa insertion after Ile505. Over aa 1‑138, human X11‑ gamma shares 62% aa identity with mouse X11‑ gamma.

Long Name

Amyloid Beta [A4] Precursor Protein-binding, Family A, Member 3

Alternate Names

MINT3, X11L2

Entrez Gene IDs

9546 (Human); 57267 (Mouse); 83611 (Rat)

Gene Symbol

APBA3

UniProt

O96018 (Human)

Additional APBA3 Products

Product Documents for Human APBA3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human APBA3 Antibody

For research use only

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