Human APJ APC-conjugated Antibody

This product is in the process of discontinuation. A recombinant monoclonal replacement is available.
New Product FAB8561A.
(1 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Stains human APJ transfectants in flow cytometry but not the parental cell line.
  • Source
    Monoclonal Mouse IgG3 Clone # 72133
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with APJ
    Met1-Asp380
    Accession # P35414
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of APJ in A172 Human Glioblastoma Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human APJ APC‑conjugated Monoclonal Antibody (Catalog # FAB856A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: APJ

APJ, also known as Apelin Receptor, GPCR-34 and AGTRL-1, is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, Apelin. APJ can also function as a co-receptor for HIV.

  • Long Name:
    Apelin Receptor/G-protein Coupled Receptor APJ
  • Entrez Gene IDs:
    187 (Human); 23796 (Mouse); 83518 (Rat)
  • Alternate Names:
    AGTRL1; AGTRL1HG11; angiotensin II receptor-like 1; Angiotensin receptor-like 1; apelin receptor; APJ (apelin) receptor; APJ receptor; APJ; APJFLJ96609; APJR; APLNR; FLJ90771; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11 orphan receptor; HG11; MGC45246
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. APELIN promotes hematopoiesis from human embryonic stem cells.
    Blood, 2012;119(26):6243-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG3 APC-conjugated Antibody

Ctrl IC007A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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Read what people are saying who have used Human APJ APC-conjugated Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Human Anonymous 12/15/2016
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Summary

ApplicationFlow Cytometry
Sample TestedInduced pluripotent stem cells,mesoderm
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAntibody was used to isolated mesoderm lineage cells from human pluripotent cells.

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