Home / APJ / Human APJ APC-conjugated Antibody

Human APJ APC-conjugated Antibody

Catalog #: FAB856A
1 Citation 1 Review Datasheet / COA / SDS
Newer Version Available: FAB8561A
NEW
This product is in the process of discontinuation. A recombinant monoclonal replacement is available

Discontinued Product

FAB856A has been discontinued and is replaced by FAB8561A.

Detection of APJ in A172 Human Glioblastoma Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (1)
FAQs
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APJ APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human APJ transfectants in flow cytometry but not the parental cell line.
Source
Monoclonal Mouse IgG3 Clone # 72133
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with APJ
Met1-Asp380
Accession # P35414
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of APJ antibody in A172 Human Glioblastoma Cell Line antibody by Flow Cytometry. View Larger

Detection of APJ in A172 Human Glioblastoma Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human APJ APC-conjugated Monoclonal Antibody (Catalog # FAB856A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: APJ

APJ, also known as Apelin Receptor, GPCR-34 and AGTRL-1, is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, Apelin. APJ can also function as a co-receptor for HIV.

Long Name
Apelin Receptor/G-protein Coupled Receptor APJ
Entrez Gene IDs
187 (Human); 23796 (Mouse); 83518 (Rat)
Alternate Names
AGTRL1; AGTRL1HG11; angiotensin II receptor-like 1; Angiotensin receptor-like 1; apelin receptor; APJ (apelin) receptor; APJ receptor; APJ; APJFLJ96609; APJR; APLNR; FLJ90771; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11 orphan receptor; HG11; MGC45246

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human APJ APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. APELIN promotes hematopoiesis from human embryonic stem cells.
    Blood, 2012-05-18;119(26):6243-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human APJ APC-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human APJ APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human APJ APC-conjugated Antibody
By Anonymous on 12/15/2016
Application: Flow Sample Tested: Induced pluripotent stem cells,mesoderm Species: Human

Antibody was used to isolated mesoderm lineage cells from human pluripotent cells.