Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Apolipoprotein B100/ApoB100 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Apoliprotein B100
Ile3374-Val3600
Accession # AAP72970

Specificity

Detects human Apoliprotein B100 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Apolipoprotein B100/ApoB100 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Apolipoprotein B100/ApoB100

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein B100/ApoB100

ApoB100 is a major serum apoliprotein synthesized by the liver. It is an important constituent of LDL, VLDL and IDL particles. It interacts with the LDL receptor and is involved in the delivery of cholesterol to tissues. Within the peptide sequence of the immunogen, human and mouse ApoB100 share 74% amino acid sequence identity. The sequence within the immunogen is absent in ApoB48, an alternative splice isoform that is exclusively synthesized by the gut. ApoB48 contains the amino terminal 47% of ApoB100 and lacks the LDL receptor region.

Alternate Names

apoB-100;apoB-48;Apolipoprotein B-100;FCHL2;FLDB;LDLCQ4

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Gene Symbol

APOB

UniProt

AAP72970 (Human)

Additional Apolipoprotein B100/ApoB100 Products

Product Documents for Human Apolipoprotein B100/ApoB100 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Apolipoprotein B100/ApoB100 Biotinylated Antibody

For research use only

Related Research Areas

Obesity

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