Human Apolipoprotein B100/ApoB100 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Apolipoprotein B100/ApoB100 by Western Blot.
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Product Details
Citations (3)
Supplemental Products

Human Apolipoprotein B100/ApoB100 Antibody Summary

Species Reactivity
Detects human ApoB100 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human ApoB100
Accession # AAP72970
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Apolipoprotein B100/ApoB100 antibody by Western Blot. View Larger

Detection of Human Apolipoprotein B100/ApoB100 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B100/ApoB100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B100/ApoB100 at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B100/ApoB100

ApoB100 is a major serum Apolipoprotein synthesized by the liver. It is an important constituent of LDL, VLDL and IDL particles. It interacts with the LDL receptor and is involved in the delivery of cholesterol to tissues. Within the peptide sequence of the immunogen, human and mouse ApoB100 share 74% amino acid sequence identity. The sequence within the immunogen is absent in ApoB48, an alternative splice isoform that is exclusively synthesized by the gut. ApoB48 contains the amino terminal 47% of ApoB100 and lacks the LDL receptor region.

Entrez Gene IDs
338 (Human); 238055 (Mouse); 54225 (Rat)
Alternate Names
apoB-100;apoB-48;Apolipoprotein B-100;FCHL2;FLDB;LDLCQ4; Apolipoprotein B100

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Citations for Human Apolipoprotein B100/ApoB100 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lipoprotein(a) Cellular Uptake Ex�Vivo and Hepatic Capture In�Vivo Is Insensitive to PCSK9 Inhibition With Alirocumab
    Authors: K Chemello, S Beeské, TT Trang Tran, V Blanchard, EF Villard, B Poirier, JC Le Bail, G Dargazanli, S Ho-Van-Gui, D Boulay, O Bergis, MP Pruniaux, M Croyal, P Janiak, E Guillot, G Lambert
    JACC Basic Transl Sci, 2020;5(6):549-557.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis
    Authors: S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran
    Physiol Rep, 2017;5(14):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Functional Assay
  3. MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells.
    Authors: Shirasaki T, Honda M, Shimakami T, Horii R, Yamashita T, Sakai Y, Sakai A, Okada H, Watanabe R, Murakami S, Yi M, Lemon S, Kaneko S
    J Virol, 2013;87(9):5270-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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