Human ATG10 Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: ATG10

ATG10 (autophagy-related protein 10; also APG10-like) is a ubiquitous 28 kDa member of the ATG10 family of proteins. It serves as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG12 to ATG5. This ATG5:ATG12 heterodimer subsequently associates non‑covalently with an ATG16 multimer to generate an autophagosome. Human ATG10 is 220 amino acids in length and contains an active site at Cys166 that forms a thiol ester bond with the C-terminal Gly of ATG12. There are multiple isoform variants. One shows a deletion of aa 37‑72, a second contains an alternate start site at Met43 that is accompanied by a 39 aa substitution for aa 152‑220, and a third shows a 53 aa substitution for aa 73‑220. Over amino acids 1‑190, human ATG10 shares 79% aa identity with mouse ATG10.

Product Datasheets

Related Research Areas

• Autophagy