ATG10 (autophagy-related protein 10; also APG10-like) is a ubiquitous 28 kDa member of the ATG10 family of proteins. It serves as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG12 to ATG5. This ATG5:ATG12 heterodimer subsequently associates non‑covalently with an ATG16 multimer to generate an autophagosome. Human ATG10 is 220 amino acids in length and contains an active site at Cys166 that forms a thiol ester bond with the C-terminal Gly of ATG12. There are multiple isoform variants. One shows a deletion of aa 37‑72, a second contains an alternate start site at Met43 that is accompanied by a 39 aa substitution for aa 152‑220, and a third shows a 53 aa substitution for aa 73‑220. Over amino acids 1‑190, human ATG10 shares 79% aa identity with mouse ATG10.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met1-Thr190
Accession # Q9H0Y0
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human ATG10 Antibody
Detection of Human ATG10 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ATG10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5464) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG10 at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Applications for Human ATG10 Antibody
Western Blot
Sample: A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ATG10
Long Name
Alternate Names
Gene Symbol
UniProt
Additional ATG10 Products
Product Documents for Human ATG10 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human ATG10 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars