Human B7-2/CD86 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB141P-100
FAB141P-025
Detection of B7‑2/CD86 in Human Blood Monocytes by Flow Cytometry.
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Citations (6)
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Human B7-2/CD86 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 37301
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of B7‑2/CD86 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of B7‑2/CD86 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human B7‑2/CD86 PE‑conjugated Monoclonal Antibody (Catalog # FAB141P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-2/CD86

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon-gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

References
  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs
942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)
Alternate Names
Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86

Product Datasheets

Citations for Human B7-2/CD86 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Regulation of type I interferon responses by mitochondria-derived reactive oxygen species in plasmacytoid dendritic cells
    Authors: Z Agod, T Fekete, MM Budai, A Varga, A Szabo, H Moon, I Boldogh, T Biro, A Lanyi, A Bacsi, K Pazmandi
    Redox Biol, 2017;13(0):633-645.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors
    Authors: KP Bene, DW Kavanaugh, C Leclaire, AP Gunning, DA MacKenzie, A Wittmann, ID Young, N Kawasaki, E Rajnavolgy, N Juge
    Front Microbiol, 2017;8(0):321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. The antiviral immune response in human conventional dendritic cells is controlled by the mammalian target of rapamycin.
    Authors: Fekete T, Pazmandi K, Szabo A, Bacsi A, Koncz G, Rajnavolgyi E
    J Leukoc Biol, 2014;96(4):579-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Oxidation of ovarian epithelial cancer cells by hypochlorous acid enhances immunogenicity and stimulates T cells that recognize autologous primary tumor.
    Authors: Chiang CL, Ledermann JA, Aitkens E, Benjamin E, Katz DR, Chain BM
    Clin. Cancer Res., 2008;14(15):4898-907.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. Eosinophils induce DC maturation, regulating immunity.
    Authors: Lotfi R, Lotze MT
    J. Leukoc. Biol., 2007;83(3):456-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  6. Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates.
    Authors: Kaur K, Chowdhury S, Greenspan NS, Schreiber JR
    Blood, 2007;110(8):2948-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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