Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant murine BAFF, and less than 1% cross‑reactivity with recombinant human (rh) APRIL, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTNF‑ alpha, rhTRAIL, rhTRANCE, and rhVEGI is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human BAFF/BLyS/TNFSF13B Ala134-Leu285 Accession # Q9Y275
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.60 EU per 1 μg of the antibody by the LAL method.
Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF)
Measured by its ability to neutralize BAFF/BLyS/TNFSF13B-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 3-12 ng/mL in the presence of 5 ng/mL Recombinant Human BAFF/BLyS/TNFSF13B and 1 µg/mL Goat F(ab')2 Anti-mouse IgM.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody. In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND50 is typically 3‑12 ng/mL.
BAFF/BLyS/TNFSF13B in Human Spleen. BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).
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Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
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Batten, M. et al. (2000) J. Exp. Med. 192:1453.
Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
B cell Activating Factor
Entrez Gene IDs:
10673 (Human); 24099 (Mouse); 498666 (Rat)
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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