Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody

Clone 137314 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
IC1241P
Detection of BAFF/BLyS/TNFSF13B in Human PBMCs by Flow Cytometry.
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Citations (2)
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Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) TL1A/TNSF15 is observed and no cross-reactivity with rhAPRIL, rhTNF-alpha, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTRAIL, rhTRANCE, or rhTWEAK is observed.
Source
Monoclonal Mouse IgG1 Clone # 137314
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human BAFF/BLyS/TNFSF13B
Ala81-Leu285
Accession # Q9Y275
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of BAFF/BLyS/TNFSF13B antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of BAFF/BLyS/TNFSF13B in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin (filled histogram) or resting (open histogram), were stained with Mouse Anti-Human BAFF/BLyS/TNFSF13B PE-conjugated Monoclonal Antibody (Catalog # IC1241P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: BAFF/BLyS/TNFSF13B

BAFF, also known as TALL-1, BLyS, and THANK, is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between Arg133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF Receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6 - 8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

References
  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
  4. Liu, Y. et al. (2002) Cell 108:383.
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L.G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Long Name
B cell Activating Factor
Entrez Gene IDs
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Product Datasheets

Citations for Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma.
    Authors: Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Br. J. Dermatol., 2012;167(2):359-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha.
    Authors: Nishio M, Endo T, Tsukada N, Ohata J, Kitada S, Reed JC, Zvaifler NJ, Kipps TJ
    Blood, 2005;106(3):1012-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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