Human BANK1 Antibody

Catalog # Availability Size / Price Qty
AF8005
AF8005-SP
Detection of Human BANK1 by Western Blot.
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Human BANK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BANK1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human BANK1
Ser480-His785 (Cys650Arg)
Accession # Q8NDB2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human BANK1 by Western Blot. View Larger

Detection of Human BANK1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BANK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BANK1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BANK1

BANK1 (B cell scaffold protein with ANKyrin repeats) is a cytoplasmic scaffold protein containing ankyrin repeats. Although its predicted MW is 85 kDa, due to its highly acidic nature, it runs anomalously at 97-105 kDa in SDS-PAGE. It is expressed in select cell types, including mature B cells and pancreatic islet beta ‑cells. In B cells, following BCR activation, IP3 is generated and BANK1 is phosphorylated by BCR-associated Syk (Spleen Tyr kinase). At this point, phosphorylated BANK1 subsequently interacts with both cytosolic Lyn (Lck/Yes-related Novel tyrosine kinase) and IP3R, leading to an IP3 receptor highly sensitive to IP3. Increased cellular IP3 binding to IP3R stimulates the release of calcium from intracellular stores. BANK1 is also posited to participate in the regulation of IgM production, its role in this case being that of a negative modulator of secretion. Human BANK1 is 785 amino acids (aa) in length. It contains an IP3R interaction region (aa 1-154), followed by a DBB domain (aa 200-327) that is involved in dimerization, and two consecutive ANK repeats (aa 345-408). There are at least three isoform variants. One utilizes an alternative start site at Met31, a second contains an eight aa substitution for aa 1-23, and a third shows a deletion of aa 24-156. BANK1 is known to form homo- and heterodimers with its isoforms, and a Arg61 to His61 transition is reported to be correlated with reduced homooligomer formation. Over aa 480-785, human BANK1 shares 73% aa sequence identity with mouse BANK1.

Long Name
B-cell Scaffold Protein with Ankyrin Repeats 1
Entrez Gene IDs
55024 (Human); 242248 (Mouse); 365948 (Rat)
Alternate Names
BANK1; BANKFLJ20706B-cell scaffold protein with ankyrin repeats; B-cell scaffold protein with ankyrin repeats 1; FLJ34204

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