Detection of Human BANK1 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BANK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BANK1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
BANK1 (B cell scaffold protein with ANKyrin repeats) is a cytoplasmic scaffold protein containing ankyrin repeats. Although its predicted MW is 85 kDa, due to its highly acidic nature, it runs anomalously at 97-105 kDa in SDS-PAGE. It is expressed in select cell types, including mature B cells and pancreatic islet beta -cells. In B cells, following BCR activation, IP 3 is generated and BANK1 is phosphorylated by BCR-associated Syk (Spleen Tyr kinase). At this point, phosphorylated BANK1 subsequently interacts with both cytosolic Lyn (Lck/Yes-related Novel tyrosine kinase) and IP 3 R, leading to an IP 3 receptor highly sensitive to IP 3. Increased cellular IP 3 binding to IP 3 R stimulates the release of calcium from intracellular stores. BANK1 is also posited to participate in the regulation of IgM production, its role in this case being that of a negative modulator of secretion. Human BANK1 is 785 amino acids (aa) in length. It contains an IP 3 R interaction region (aa 1-154), followed by a DBB domain (aa 200-327) that is involved in dimerization, and two consecutive ANK repeats (aa 345-408). There are at least three isoform variants. One utilizes an alternative start site at Met31, a second contains an eight aa substitution for aa 1-23, and a third shows a deletion of aa 24-156. BANK1 is known to form homo- and heterodimers with its isoforms, and a Arg61 to His61 transition is reported to be correlated with reduced homooligomer formation. Over aa 480-785, human BANK1 shares 73% aa sequence identity with mouse BANK1.
B-cell Scaffold Protein with Ankyrin Repeats 1
Entrez Gene IDs:
55024 (Human); 242248 (Mouse); 365948 (Rat)
BANK1; BANKFLJ20706B-cell scaffold protein with ankyrin repeats; B-cell scaffold protein with ankyrin repeats 1; FLJ34204
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