Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 841702
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Product Specifications

Immunogen

E. coli-derived recombinant human BATF3
Met1-Arg127
Accession # Q9NR55

Specificity

Detects human BATF3 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human BATF3 Alexa Fluor® 488‑conjugated Antibody

Detection of BATF3 in Human PBMC Monocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c APC-conjugated Monoclonal Antibody (FAB1777A) and either (A) Mouse Anti-Human BATF3 Alexa Fluor® 488-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC7437G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (IC0041G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of BATF3 in Jurkat cells by Flow Cytometry

Jurkat cells were stained with Mouse Anti-Human BATF3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7437G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human BATF3 Alexa Fluor® 488‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005); Jurkat human T cell leukemia cell line fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer kit (FC012)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BATF3

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.

Long Name

Basic Leucine Zipper Transcription Factor, ATF-like 3

Alternate Names

JDP1, JUNDM1, SNFT

Entrez Gene IDs

55509 (Human); 381319 (Mouse); 60462 (Rat)

Gene Symbol

BATF3

UniProt

Additional BATF3 Products

Product Documents for Human BATF3 Alexa Fluor® 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BATF3 Alexa Fluor® 488‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail