Human BATF3 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human BATF3 in ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 841702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BATF3
    Met1-Arg127
    Accession # Q9NR55
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of BATF3 in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB1777A) and either (A) Mouse Anti-Human BATF3 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC7437G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BATF3

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.

  • Long Name:
    Basic Leucine Zipper Transcription Factor, ATF-like 3
  • Entrez Gene IDs:
    55509 (Human); 381319 (Mouse); 60462 (Rat)
  • Alternate Names:
    basic leucine zipper transcription factor, ATF-like 3; basic leucine zipper transcriptional factor ATF-like 3; BATF3; B-ATF-3; FLJ36352; FLJ37535,21 kDa small nuclear factor isolated from T-cells; JDP1; Jun dimerization protein 1; Jun dimerization protein p21SNFT; JUNDM1; SNFT; SNFT21-kD small nuclear factor isolated from T cells
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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