Intracellular Staining by Flow Cytometry
|Detection of BATF3 in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB1777A) and either (A) Mouse Anti-Human BATF3 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC7437G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.