Human BATF3 Antibody Summary
Accession # Q9NR55
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of BATF3 in Human (PBMC) Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c PE-conjugated Monoclonal Antibody (Catalog # FAB1777P) and either (A) Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.
Citation for Human BATF3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Genomic programming of IRF4-expressing human Langerhans cells
Authors: S Sirvent, AF Vallejo, J Davies, K Clayton, Z Wu, J Woo, J Riddell, VK Chaudhri, P Stumpf, LA Nazlamova, G Wheway, M Rose-Zeril, J West, M Pujato, X Chen, CH Woelk, B MacArthur, M Ardern-Jon, PS Friedmann, MT Weirauch, H Singh, ME Polak
Nat Commun, 2020;11(1):313.
Sample Types: Whole Cells
Applications: Flow Cytometry
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