Human Bcl-9 Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Bcl-9

Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.

Product Datasheets

Related Research Areas

• Stem Cell Transcription Factor Regulators
• Transcriptional Co-Activators
• Wnt Intracellular Signaling