Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Bcl-9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bcl-9
Met1009-Gly1328
Accession # O00512

Specificity

Detects endogenous human Bcl-9 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Bcl‑9 Antibody

Detection of Human Bcl‑9 antibody by Western Blot.

Detection of Human Bcl‑9 by Western Blot.

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bcl-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3996) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Bcl-9 at approximately 150 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human Bcl‑9 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-9

Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.

Long Name

B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling

Alternate Names

Bcl9, BCL9L, LGS

Entrez Gene IDs

607 (Human); 77578 (Mouse); 310704 (Rat)

Gene Symbol

BCL9

UniProt

O00512

Additional Bcl-9 Products

Product Documents for Human Bcl‑9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Bcl‑9 Antibody

For research use only

Citations for Human Bcl‑9 Antibody

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