BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Blockade of Receptor-ligand Interaction
Cited:
Western Blot, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 335004
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BCMA
Met1-Ala54
Accession # Q02223
Met1-Ala54
Accession # Q02223
Specificity
Detects human BCMA in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross‑reactivity with recombinant mouse (rm) BCMA or rmTACI.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human BCMA/TNFRSF17 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2 - 0.8 μg/mL of this antibody will block 50% of the binding of 500 ng/mL of recombinant human APRIL to immobilized recombinant human BCMA coated at 1 μg/mL (100 μL/well). At 5 μg/mL, this antibody will block >90% of the binding.
Western Blot
1 µg/mL
Sample: Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)
Sample: Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)
Reviewed Applications
Read 5 reviews rated 4.2 using MAB193 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: BCMA/TNFRSF17
References
- Madry, C. et al. (1998) Int. Immunol. 10:1693.
- Gras, M. et al. (1995) Int. Immunol. 7:1093.
- Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
- Marsters, S. et al. (2000) Curr. Biol. 10:785.
- Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Alternate Names
CD269, TNFRSF13A, TNFRSF17
Gene Symbol
TNFRSF17
UniProt
Additional BCMA/TNFRSF17 Products
Product Documents for Human BCMA/TNFRSF17 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human BCMA/TNFRSF17 Antibody
For research use only
Citations for Human BCMA/TNFRSF17 Antibody
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Customer Reviews for Human BCMA/TNFRSF17 Antibody (5)
4.2 out of 5
5 Customer Ratings
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Application: ELISASample Tested: SerumSpecies: HumanVerified Customer | Posted 12/05/2024Ideal for capture antibody in ELISA assay. Provided low background levels.
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 06/10/2020
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 02/07/2020Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
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Application: LuminexSample Tested: SerumSpecies: HumanVerified Customer | Posted 11/08/2018
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/07/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
