NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Equine
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 347112
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant human pro-beta -NGF
Glu19-Ala241
Accession # P01138
Glu19-Ala241
Accession # P01138
Specificity
Detects human pro-beta -NGF in Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with recombinant mouse (rm) beta -NGF, rr beta -NGF, rhBDNF, rhNT-3, or rhNT-4.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Human Pro beta ‑NGF Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Pro beta -NGF. This antibody does not detect mature Recombinant Human beta -NGF.
Sample: Recombinant Human Pro beta -NGF. This antibody does not detect mature Recombinant Human beta -NGF.
Reviewed Applications
Read 1 review rated 3 using MAB2562 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: beta-NGF
Long Name
beta Nerve Growth Factor
Alternate Names
betaNGF, NGFB
Gene Symbol
NGF
UniProt
Additional beta-NGF Products
Product Documents for Human Pro beta ‑NGF Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Pro beta ‑NGF Antibody
For research use only
Related Research Areas
Citations for Human Pro beta ‑NGF Antibody
Powered by Bioz
Customer Reviews for Human Pro beta ‑NGF Antibody (1)
3 out of 5
1 Customer Rating
Have you used Human Pro beta ‑NGF Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Simple WesternSample Tested: Serum and Synovial fluidSpecies: EquineVerified Customer | Posted 06/03/2020Primary ab: MAB2562: 10ug/ml Secondary ab: HAF005, diluted 1:50
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
