Detection of BLAME/SLAMF8 in Human Monocytes by Flow Cytometry.
Human monocytes, untreated (filled histogram, gold) or treated with Recombinant Human IFN-gamma (Catalog # 285-IF; filled histogram, orange), were stained with Mouse Anti-Human BLAME/SLAMF8 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB19072F) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%‑26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).
McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.
Entrez Gene IDs:
56833 (Human); 74748 (Mouse); 289237 (Rat)
B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8
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Flow Cytometry: Human BLAME/SLAMF8 Fluorescein-conjugated Antibody [FAB19072F].
Peripheral blood monocytes
Other Experimental Details
Other Experimental Details
Gated on monocyte FSC-A and SSC-A population. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Isotype control shaded grey, SLAMF8 in open histogram. Specificity: Specific Sensitivity: Sensitive Buffer: 2% Human Serum, 0.5 mM EDTA in PBS Dilution: 1/100