Human BLAME/SLAMF8 PE-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human BLAME/SLAMF8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SLAMF7 or rhSLAMF6 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 250014
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
    Ala23-Asp233
    Accession # Q9P0V8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of BLAME/SLAMF8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes untreated (light orange filled histogram) or treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF, dark orange filled histogram) were stained with Mouse Anti-Human BLAME/SLAMF8 PE‑conjugated Monoclonal Antibody (Catalog # FAB19072P) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BLAME/SLAMF8

BLAME (B-Lymphocyte Activator Macrophage Expressed), also known as SLAM Family Member 8 (SLAMF8), is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%-26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

  • References:
    1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
    2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
    3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.
  • Entrez Gene IDs:
    56833 (Human); 74748 (Mouse); 289237 (Rat)
  • Alternate Names:
    B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 39  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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