Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 250014
View all BLAME/SLAMF8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
Ala23-Asp233
Accession # Q9P0V8

Specificity

Detects human BLAME/SLAMF8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SLAMF7 or rhSLAMF6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BLAME/SLAMF8 PE‑conjugated Antibody

Detection of BLAME/SLAMF8 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of BLAME/SLAMF8 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes untreated (light orange filled histogram) or treated with Recombinant Human IFN-gamma (Catalog # 285-IF, dark orange filled histogram) were stained with Mouse Anti-Human BLAME/SLAMF8 PE-conjugated Monoclonal Antibody (Catalog # FAB19072P) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human BLAME/SLAMF8 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF)

Reviewed Applications

Read 1 review rated 5 using FAB19072P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BLAME/SLAMF8

BLAME (B-Lymphocyte Activator Macrophage Expressed), also known as SLAM Family Member 8 (SLAMF8), is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%-26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

References

  1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

Long Name

SLAM Family Member 8

Alternate Names

CD353, SBBI42, SLAMF8

Entrez Gene IDs

56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)

Gene Symbol

SLAMF8

UniProt

Q9P0V8

Additional BLAME/SLAMF8 Products

Product Documents for Human BLAME/SLAMF8 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BLAME/SLAMF8 PE‑conjugated Antibody

For research use only

Customer Reviews for Human BLAME/SLAMF8 PE‑conjugated Antibody (1)

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  • Human BLAME/SLAMF8 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human peripheral CD11c+ dendritic cells
    Species: Human
    Verified Customer | Posted 06/24/2018

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