Human BMP-4 MAb (Clone 66119)

Catalog # Availability Size / Price Qty
Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody.
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Product Details
Citations (16)
Supplemental Products
Reviews (4)

Human BMP-4 MAb (Clone 66119) Summary

Species Reactivity
Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.
Monoclonal Mouse IgG2B Clone # 66119
Protein A or G purified from ascites
Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Accession # P12644
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human BMP‑4 (Catalog # 314-BP)
Measured by its ability to neutralize BMP‑4-induced alkaline phosphatase production in the C2C12 mouse myoblast cell line. Katagiri, T. et al. (1994) J. Cell Biol. 127:1755. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 500 ng/mL Recombinant Human BMP‑4 and 2 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).

  1. Zhang, P. et al., 2008, Blood 111:1933.
  2. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.
  3. Simic, P.  and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al., 2004, Stem Cells 22:457.
  5. Frank, D.B. et al., 2005, Circ. Res. 97:496.
  6. Wozney, J. et al., 1988, Science 242:1528.
  7. Cui, Y. et al., 1998, EMBO J. 17:4735.
  8. Cui, Y. et al., 2001, Genes Dev. 15:2797.
  9. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.
  11. Chen, D. et al., 2004, Growth Factors 22:233.
  12. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.
  13. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.
  15. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.
Long Name
Bone Morphogenetic Protein 4
Entrez Gene IDs
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names
BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

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Citations for Human BMP-4 MAb (Clone 66119)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Roles of Gremlin 1 and Gremlin 2 in regulating ovarian primordial to primary follicle transition
    Authors: Eric E. Nilsson, Ginger Larsen, Michael K. Skinner
  2. Comparison of newly developed anti-bone morphogenetic protein 4 llama-derived antibodies with commercially available BMP4 inhibitors
    Authors: Silvia Calpe, Ana C. P. Correia, Maria del Carmen Sancho-Serra, Kausilia K. Krishnadath
  3. Ibrutinib Inhibits Angiogenesis and Tumorigenesis in a BTK-Independent Manner
    Authors: J Liu, Z Liu, J Zhang, X Chen, J Chen, L Sui, J Yu
    Pharmaceutics, 2022-09-05;14(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Furin, a transcriptional target of NKX2-5, has an essential role in heart development and function
    Authors: L Dupays, N Towers, S Wood, A David, DJ Stuckey, T Mohun
    PLoS ONE, 2019-03-06;14(3):e0212992.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma
    Authors: AK Patel, K Vipparthi, V Thatikonda, I Arun, S Bhattachar, R Sharan, P Arun, S Singh
    Oncogenesis, 2018-10-05;7(10):78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer
    Authors: N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti
    Oncogene, 2018-04-26;37(29):4013-4032.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells
    Authors: C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat
    J. Biol. Chem., 2017-07-26;0(0):.
    Species: Human, Ovine
    Sample Types: Cell Culture Supernates, Recombinant Protein
    Applications: Immunoprecipitation, Western Blot
  8. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ
    BMC Cell Biol., 2009-03-19;10(0):20.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  9. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008-07-29;112(8):3154-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis.
    Authors: Kane R, Godson C, O'Brien C
    Mol. Vis., 2008-06-20;14(0):1138-48.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells.
    Authors: Zode GS, Clark AF, Wordinger RJ
    Invest. Ophthalmol. Vis. Sci., 2007-11-01;48(11):5058-67.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions.
    Authors: Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH
    Ann. Neurol., 2007-09-01;62(3):288-300.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
    Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR
    J. Pathol., 2006-06-01;209(2):190-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation.
    Authors: Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C
    Mol. Cell. Biol., 2003-08-01;23(16):5664-79.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  15. Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
    Authors: van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
    Gastroenterology, 2001-08-01;121(2):317-28.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Bone morphogenetic protein 2 controls iron homeostasis in mice independent of Bmp6
    Authors: Susanna Canali, Chia-Yu Wang, Kimberly B. Zumbrennen-Bullough, Abraham Bayer, Jodie L. Babitt
    American Journal of Hematology


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