Human BMP-4 Quantikine ELISA Kit

  (12 citations)     
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Product Details
Assay Procedure
Citations (12)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Bone Extracts (50 uL)
  • Sensitivity
    3.68 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Bone Extracts)
  • Specificity
    Natural and recombinant human BMP-4
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC50 , Quantikine Immunoassay Control Set 605 for Human BMP-4 - Please Inquire
Product Summary
The Quantikine Human BMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure BMP-4 levels in bone tissue extracts and cell culture supernates. It contains NS0-expressed recombinant human BMP-4 and antibodies raised against the recombinant factor.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Bone Extracts
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 301 719 1351 289 734 1429
Standard Deviation 15.9 22.5 60.1 16.9 42.7 75.4
CV% 5.3 3.1 4.4 5.8 5.8 5.3

Recovery

The recovery of recombinant human BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
2 M Guanidine-HCI (n=2) 96 93-103
2 M Urea (n=2) 97 91-105
Cell Culture Media (n=8) 100 91-109
Linearity
The following samples were spiked with rhBMP-4 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess linearity of the assay.
 BMP-4 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-4

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

  • Long Name:
    Bone Morphogenetic Protein 4
  • Entrez Gene IDs:
    652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
  • Alternate Names:
    BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours. PROTECT FROM LIGHT.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Sample Type
  1. HTRA1 and TGF-?1 Concentrations in the Aqueous Humor of Patients With Neovascular Age-Related Macular Degeneration
    Authors: GM Tosi, E Caldi, G Neri, E Nuti, D Marigliani, S Baiocchi, C Traversi, G Cevenini, A Tarantello, F Fusco, F Nardi, M Orlandini, F Galvagni
    Invest. Ophthalmol. Vis. Sci, 2017;58(1):162-167.
    Species: Human
    Sample Type: Aqueous humor
  2. The Axolotl Fibula as a Model for the Induction of Regeneration across Large Segment Defects in Long Bones of the Extremities.
    Authors: Chen X, Song F, Jhamb D, Li J, Bottino M, Palakal M, Stocum D
    PLoS ONE, 2015;10(6):e0130819.
    Species: Human
    Sample Type: Recombinant Protein
  3. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response.
    Authors: Chapellier M, Bachelard-Cascales E, Schmidt X, Clement F, Treilleux I, Delay E, Jammot A, Menetrier-Caux C, Pochon G, Besancon R, Voeltzel T, Caron de Fromentel C, Caux C, Blay J, Iggo R, Maguer-Satta V
    Stem Cell Reports, 2015;4(2):239-54.
    Species: Human
    Sample Type: Tissue Homogenates
  4. Mesenchymal stromal cells derived from the bone marrow of acute lymphoblastic leukemia patients show altered BMP4 production: correlations with the course of disease.
    Authors: Vicente Lopez A, Vazquez Garcia M, Melen G, Entrena Martinez A, Cubillo Moreno I, Garcia-Castro J, Orellana M, Gonzalez A
    PLoS ONE, 2014;9(1):e84496.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Bone morphogenetic protein-2/-4 upregulation promoted by endothelial cells in coculture enhances mouse embryoid body differentiation.
    Authors: Talavera-Adame, Dodanim, Gupta, Ankur, Kurtovic, Silvia, Chaiboonma, Kira L, Arumugaswami, Vaithili, Dafoe, Donald C
    Stem Cells Dev, 2013;22(24):3252-60.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Carrageenan reduces bone morphogenetic protein-4 (BMP4) and activates the Wnt/beta-catenin pathway in normal human colonocytes.
    Authors: Bhattacharyya S, Borthakur A, Dudeja PK, Tobacman JK
    Dig. Dis. Sci., 2007;52(10):2766-74.
    Species: Human
    Sample Type: Cell Culture Supernates
  8. Malignant transformation of multipotent muscle-derived cells by concurrent differentiation signals.
    Authors: Pollett JB, Corsi KA, Weiss KR, Cooper GM, Barry DA, Gharaibeh B, Huard J
    Stem Cells, 2007;25(9):2302-11.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. The performance of poly-epsilon-caprolactone scaffolds in a rabbit femur model with and without autologous stromal cells and BMP4.
    Authors: Savarino L, Baldini N, Greco M, Capitani O, Pinna S, Valentini S, Lombardo B, Esposito MT, Pastore L, Ambrosio L, Battista S, Causa F, Zeppetelli S, Guarino V, Netti PA
    Biomaterials, 2007;28(20):3101-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
    Authors: Herrmann S, Green J, Weber T, Scharfenberger A
    Bone, 2006;39(5):1156-63.
    Species: Human
    Sample Type: Tissue Homogenates
  11. Measurement of bone morphogenetic proteins and other growth factors in demineralized bone matrix.
    Authors: Blum B, Moseley J, Miller L, Richelsoph K, Haggard W
    Orthopedics, 2004;27(1):161-5.
    Species: Rat
    Sample Type: Tissue Homogenates
  12. Oncogenic beta-catenin is required for bone morphogenetic protein 4 expression in human cancer cells.
    Authors: Kim JS, Crooks H, Dracheva T, Nishanian TG, Singh B, Jen J, Waldman T
    Cancer Res., 2002;62(10):2744-8.
    Species: Human
    Sample Type: Cell Culture Supernates
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Quantikine Immunoassay Control Set 605 for Human BMP-4

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