Human BMP-4 Quantikine ELISA Kit

R&D Systems | Catalog # DBP400

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Bone Extracts (50 µL)

Sensitivity

3.68 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Bone Extracts)
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Product Summary for Human BMP-4 Quantikine ELISA Kit

The Quantikine Human BMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure BMP-4 levels in bone tissue extracts and cell culture supernates. It contains NS0-expressed recombinant human BMP-4 and antibodies raised against the recombinant factor.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human BMP-4

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Bone Extracts, Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 301 719 1351 289 734 1429
Standard Deviation 15.9 22.5 60.1 16.9 42.7 75.4
CV% 5.3 3.1 4.4 5.8 5.8 5.3

Recovery for Human BMP-4 Quantikine ELISA Kit

The recovery of recombinant human BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
2 M Guanidine-HCI (n=2) 96 93-103
2 M Urea (n=2) 97 91-105
Cell Culture Media (n=8) 100 91-109

Linearity

The following samples were spiked with rhBMP-4 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess linearity of the assay.

Human BMP-4 ELISA Linearity

Scientific Data Images for Human BMP-4 Quantikine ELISA Kit

Human BMP-4 ELISA Standard Curve

Human BMP-4 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BMP-4

 

BMP-4 forms homodimers or heterodimers with BMP-2 and signals through receptor complexes composed of type I (primarily ActRIA or BMPR-IA) and type II (primarily ActRIIA or BMPR-II) receptors. BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Gene Symbol

BMP4

Additional BMP-4 Products

Product Documents for Human BMP-4 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BMP-4 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human BMP-4 Quantikine ELISA Kit

Customer Reviews for Human BMP-4 Quantikine ELISA Kit (3)

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  • Human BMP-4 Quantikine ELISA Kit
    Name: Veronica Robert
    Sample Tested: Corneal stromal stem cell
    Verified Customer | Posted 03/01/2022
    Human BMP-4 Quantikine ELISA Kit DBP400
  • Human BMP-4 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: human blood treated with DAPA
    Verified Customer | Posted 11/22/2021
    Human BMP-4 Quantikine ELISA Kit DBP400
  • Human BMP-4 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: human retinal progenitor cells
    Verified Customer | Posted 09/23/2021
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Protocols

View specific protocols for Human BMP-4 Quantikine ELISA Kit (DBP400):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours. PROTECT FROM LIGHT.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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