hh Human BMP-7 Quantikine ELISA Kit DBP700: R&D Systems
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Bone Extracts (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
  • Sensitivity
    7.83 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Bone Extracts, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human BMP-7
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC51, Quantikine Immunoassay Control Set 841 for Human BMP-7 - Please Inquire
Product Summary
The Quantikine Human BMP-7 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-7 in bone tissue extracts, cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human BMP-7 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BMP-7 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-7.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean37081412413567521148
Standard Deviation20.626.840.927.554.581.5
CV%5.63.33.37.77.27.1

Bone Extracts, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean36578311943367181110
Standard Deviation22.135.980.731.964.986.4
CV%6.14.66.89.597.8

Recovery

The recovery of BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Bone Extraction Solution (n=2) 96 88-102
Cell Culture Media (n=4) 98 90-114
EDTA Plasma (n=4) 100 93-105
Heparin Plasma (n=4) 106 96-115
Serum (n=4) 100 90-105
Urine (n=4) 97 90-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of BMP-7 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human BMP-7 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-7

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

    • Long Name
      Bone Morphogenetic Protein 7
    • Entrez Gene IDs
      655 (Human); 12162 (Mouse); 85272 (Rat);
    • Alternate Names
      BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    8 Citations: Showing 1 - 8
    Filter your results:

    Species
    Sample Type
    1. Serum Markers of Epithelial Mesenchymal Transition as Predictors of HCV-induced Liver Fibrosis, Cirrhosis and Hepatocellular Carcinoma.
      Authors: Zoheiry M, Hasan S, El-Ahwany E, Nagy F, Taleb H, Nosseir M, Magdy M, Meshaal S, El-Talkawy M, Raafat I
      Electron Physician, 2015;7(8):1626-37.
      Species: Human
      Sample Type: Serum
    2. Circulating bone morphogenetic protein-7 and transforming growth factor-beta1 are better predictors of renal end points in patients with type 2 diabetes mellitus.
      Authors: Wong M, Perkovic V, Woodward M, Chalmers J, Li Q, Hillis G, Yaghobian Azari D, Jun M, Poulter N, Hamet P, Williams B, Neal B, Mancia G, Cooper M, Pollock C
      Kidney Int, 2013;83(2):278-84.
      Species: Human
      Sample Type: Plasma
    3. Bone morphogenetic proteins and the polycystic ovary syndrome.
      Authors: van Houten E, Laven J, Louwers Y, McLuskey A, Themmen A, Visser J
      J Ovarian Res, 2013;6(1):32.
      Species: Human
      Sample Type: Serum
    4. Association of bone morphogenetic proteins with spinal fusion in ankylosing spondylitis.
      Authors: Chen HA, Chen CH, Lin YJ, Chen PC, Chen WS, Lu CL, Chou CT
      J. Rheumatol., 2010;37(10):2126-32.
      Species: Human
      Sample Type: Serum
    5. Bone morphogenetic protein 7 in the development and treatment of bone metastases from breast cancer.
      Authors: Buijs JT, Henriquez NV, van Overveld PG, van der Horst G, Que I, Schwaninger R, Rentsch C, ten Dijke P, Cleton-Jansen AM, Driouch K, Lidereau R, Bachelier R, Vukicevic S, Clezardin P, Papapoulos SE, Cecchini MG, Lowik CW, Van Der Pluijm G
      Cancer Res., 2007;67(18):8742-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Combination of scaffold and adenovirus vectors expressing bone morphogenetic protein-7 for alveolar bone regeneration at dental implant defects.
      Authors: Zhang Y, Song J, Shi B, Wang Y, Chen X, Huang C, Yang X, Xu D, Cheng X, Chen X
      Biomaterials, 2007;28(31):4635-42.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Bone morphogenetic protein 7 inhibits tumor growth of human uveal melanoma in vivo.
      Authors: Notting I, Buijs J, Mintardjo R, van der Horst G, Vukicevic S, Lowik C, Schalij-Delfos N, Keunen J, Van Der Pluijm G
      Invest. Ophthalmol. Vis. Sci., 2007;48(11):4882-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Bone morphogenetic protein 7 is elevated in patients with chronic liver disease and exerts fibrogenic effects on human hepatic stellate cells.
      Authors: Tacke F, Gabele E, Bataille F, Schwabe RF, Hellerbrand C, Klebl F, Straub RH, Luedde T, Manns MP, Trautwein C, Brenner DA, Scholmerich J, Schnabl B
      Dig. Dis. Sci., 2007;52(12):3404-15.
      Species: Human
      Sample Type: Plasma
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 841 for Human BMP-7

    Ctrl QC51
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Average Rating: 4.7 (Based on 3 reviews)

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      Excellent
      Anonymous 06/20/2016
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    Summary

    Sample TestedCartilage tissue
     Human BMP-7 Quantikine ELISA Kit DBP700
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    Excellent
      Anonymous 05/09/2016
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     Human BMP-7 Quantikine ELISA Kit DBP700
    : Human BMP-7 Quantikine ELISA Kit [DBP700]

    Summary

    Sample TestedBone Extracts

    Other Experimental Details

    Other Experimental DetailsVery easy to follow instructions.
     Human BMP-7 Quantikine ELISA Kit DBP700
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    Very Good
      Anonymous 05/04/2016
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     Human BMP-7 Quantikine ELISA Kit DBP700
    : Human BMP-7 Quantikine ELISA Kit [DBP700]

    Summary

    Sample Testeddemineralized bone extracts

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