Human BMP-7 Quantikine ELISA Kit

R&D Systems | Catalog # DBP700

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Bone Extracts (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (50 µL)

Sensitivity

7.83 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Bone Extracts, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human BMP-7 Quantikine ELISA Kit

The Quantikine Human BMP-7 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-7 in bone tissue extracts, cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human BMP-7 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BMP-7 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-7.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human BMP-7

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Bone Extracts, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 365 783 1194 336 718 1110
Standard Deviation 22.1 35.9 80.7 31.9 64.9 86.4
CV% 6.1 4.6 6.8 9.5 9.0 7.8

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 370 814 1241 356 752 1148
Standard Deviation 20.6 26.8 40.9 27.5 54.5 81.5
CV% 5.6 3.3 3.3 7.7 7.2 7.1

Recovery for Human BMP-7 Quantikine ELISA Kit

The recovery of BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Bone Extraction Solution (n=2) 96 88-102
Cell Culture Media (n=4) 98 90-114
EDTA Plasma (n=4) 100 93-105
Heparin Plasma (n=4) 106 96-115
Serum (n=4) 100 90-105
Urine (n=4) 97 90-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of BMP-7 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human BMP-7 ELISA Linearity

Scientific Data Images for Human BMP-7 Quantikine ELISA Kit

Human BMP-7 ELISA Cell Culture Supernate/Urine Standard Curve

Human BMP-7 ELISA Cell Culture Supernate/Urine Standard Curve

Human BMP-7 ELISA Serum/Plasma/Bone Standard Curve

Human BMP-7 ELISA Serum/Plasma/Bone Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BMP-7

 

BMP-7 (Bone morphogenetic protein 7) forms disulfide-linked homodimers and heterodimers with BMP-2 or BMP-4. It interacts with the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB. BMP-7 promotes new bone formation and nephron development, inhibits the branching of prostate epithelium, and antagonizes epithelial-mesenchymal transition (EMT). In pathological conditions, BMP-7 inhibits tumor growth and metastasis, ameliorates fibrotic damage in nephritis, and promotes neuroregeneration following brain ischemia.

Long Name

Bone Morphogenetic Protein 7

Alternate Names

BMP7, OP-1

Entrez Gene IDs

655 (Human); 12162 (Mouse); 85272 (Rat)

Gene Symbol

BMP7

Additional BMP-7 Products

Product Documents for Human BMP-7 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BMP-7 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human BMP-7 Quantikine ELISA Kit

Customer Reviews for Human BMP-7 Quantikine ELISA Kit (3)

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  • Human BMP-7 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cartilage tissue
    Verified Customer | Posted 06/20/2016
    Human BMP-7 Quantikine ELISA Kit DBP700
  • Human BMP-7 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Bone Extracts
    Verified Customer | Posted 05/09/2016
    Very easy to follow instructions.
    Human BMP-7 Quantikine ELISA Kit DBP700
  • Human BMP-7 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: demineralized bone extracts
    Verified Customer | Posted 05/04/2016
    Human BMP-7 Quantikine ELISA Kit DBP700

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Protocols

View specific protocols for Human BMP-7 Quantikine ELISA Kit (DBP700):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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