Human C-Reactive Protein/CRP Quantikine ELISA Kit

R&D Systems | Catalog # DCRP00

R&D Systems
Discontinued Product
DCRP00 has been discontinued. An alternative/replacement product is available: DCRP00B. View all C-Reactive Protein/CRP products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

0.022 ng/mL

Assay Range

0.78-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all C-Reactive Protein/CRP ELISA Kits »

Product Summary for Human C-Reactive Protein/CRP Quantikine ELISA Kit

The Quantikine Human CRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CRP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CRP.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human CRP

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human CRP in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=35)17691091214
EDTA plasma (n=35)1547104-41851082
Heparin plasma (n=35)1624108-45231138
Note: Four additional serum, EDTA plasma, and heparin plasma patient sample sets were tested and resulted in substantially higher values. The values for each donor averaged 7364 ng/mL, 12,363 ng/mL, 16,064 ng/mL, and 43,036 ng/mL.

Cell Culture Supernates - Human peripheral blood cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for levels of human CRP. No detectable levels were observed. 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 4.79 8.66 18.9 4.84 8.66 17.5
Standard Deviation 0.21 0.33 1.57 0.29 0.61 1.15
CV% 4.4 3.8 8.3 6.0 7.0 6.6

Recovery for Human C-Reactive Protein/CRP Quantikine ELISA Kit

The recovery of CRP spiked to levels throughout the range of the assay in cell culture media was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 92-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CRP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human C-Reactive Protein/CRP ELISA Linearity

Scientific Data Images for Human C-Reactive Protein/CRP Quantikine ELISA Kit

Human C-Reactive Protein/CRP ELISA Standard Curve

Human C-Reactive Protein/CRP ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a non-glycosylated protein in the Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP functions as a sensor and activator of the innate immune response (1). In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation (2). In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2 (3). CRP assembles non-covalently into a 110-120 kDa cyclical pentamer (4). Mature human CRP shares 71% and 64% amino acid (aa) sequence identity with mouse and rat CRP, respectively (5). 
CRP binds and opsonizes apoptotic cells (6-8) as well as bacteria such as S. pneumoniae (9, 10). It subsequently enhances the phagocytosis of these opsonized cells (6, 8-10). CRP additionally binds several proteins in the complement cascade including C1q, C4BP, and Factor H (8, 11-13). It enhances activation of the classical complement pathway and the deposition of C3b (9). In later stages of the response, CRP inhibits complement-mediated cell lysis through its binding to C4BP and Factor H (8, 12). These interactions induce the upregulation of complement inhibitory proteins CD46, CD59, and CD55/DAF and inhibit assembly of the membrane attack complex (MAC) (8, 14). 
CRP binds to Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells (15-17), and Fc receptors are required for the phagocytosis of CRP-opsonized target cells (6, 10, 18). CRP binding to Fc gamma RI induces Src activation which subsequently triggers the inhibitory Fc gamma RIIb and dampens the inflammatory response (15, 19). CRP additionally promotes dendritic cell maturation and humoral immunity (10). In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in coronary artery infarction, and inhibits the repair of injured vascular endothelium (7, 19, 20). 

Alternate Names

CRP

Entrez Gene IDs

1401 (Human); 12944 (Mouse); 25419 (Rat)

Gene Symbol

CRP

Additional C-Reactive Protein/CRP Products

Product Documents for Human C-Reactive Protein/CRP Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human C-Reactive Protein/CRP Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human C-Reactive Protein/CRP Quantikine ELISA Kit

Customer Reviews for Human C-Reactive Protein/CRP Quantikine ELISA Kit (4)

5 out of 5
4 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human C-Reactive Protein/CRP Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human C-Reactive Protein/CRP Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 03/22/2021
    Great kit, used frequently on EDTA plasma without issue. Would recommend.
    Human C-Reactive Protein/CRP Quantikine ELISA Kit DCRP00
  • Human C-Reactive Protein/CRP Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 10/05/2020
  • Human C-Reactive Protein/CRP Quantikine ELISA Kit
    Name: Ping Yi
    Sample Tested: Serum
    Verified Customer | Posted 10/04/2020
  • Human C-Reactive Protein/CRP Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 10/02/2017
    Robust and very easy to use assay
    Human C-Reactive Protein/CRP Quantikine ELISA Kit DCRP00

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

View specific protocols for Human C-Reactive Protein/CRP Quantikine ELISA Kit (DCRP00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits