Human C-Reactive Protein/CRP Quantikine ELISA Kit

(49 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    0.022 ng/mL
  • Assay Range
    0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human CRP
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC70, Quantikine Immunoassay Control Set 960 for Human CRP - Please Inquire
Product Summary
The Quantikine Human CRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CRP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CRP.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4.798.6618.94.848.6617.5
Standard Deviation0.210.331.570.290.611.15
CV%4.43.88.3676.6

Recovery

The recovery of CRP spiked to levels throughout the range of the assay in cell culture media was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 92-110
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CRP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human C-Reactive Protein/CRP Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
    • Entrez Gene IDs
      1401 (Human); 12944 (Mouse); 25419 (Rat);
    • Alternate Names
      C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    49 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Preliminary study of hypoxia-related cardiovascular mediator-markers in patients with end-stage renal disease with and without diabetes and the effects of haemodialysis
      Authors: A Treweeke, J Hall, S Lambie, SJ Leslie, IL Megson, SM MacRury
      PLoS ONE, 2017;12(5):e0178171.
      Species: Human
      Sample Type: Plasma
    2. Link between plasminogen activator inhibitor-1 and cardiovascular risk in chronic hepatitis C after viral clearance
      Authors: ML Chang, YS Lin, LH Pao, HC Huang, CT Chiu
      Sci Rep, 2017;7(0):42503.
      Species: Human
      Sample Type: Serum
    3. Immunogenic FEAT protein circulates in the bloodstream of cancer patients
      J Transl Med, 2016;14(1):275.
      Species: Human
      Sample Type: Plasma
    4. P2X7 Receptor Inhibition Improves CD34 T-Cell Differentiation in HIV-Infected Immunological Nonresponders on c-ART
      Authors: I Menkova-Ga, H Hocini, E Foucat, P Tisserand, L Bourdery, C Delaugerre, C Benne, Y Lévy, JD Lelièvre
      PLoS Pathog, 2016;12(4):e1005571.
      Species: Human
      Sample Type: Plasma
    5. Circadian variations in clinical symptoms and concentrations of inflammatory cytokines, melatonin, and cortisol in polymyalgia rheumatica before and during prednisolone treatment: a controlled, observational, clinical experimental study
      Arthritis Res Ther, 2016;18(1):174.
      Species: Human
      Sample Type: Plasma
    6. Effects of nateglinide and rosiglitazone on pancreatic alpha- and beta-cells, GLP-1 secretion and inflammatory markers in patients with type 2 diabetes: randomized crossover clinical study.
      Authors: Tostes G, Cunha M, Fukui R, Correia M, Rocha D, Dos Santos R, da Silva M
      Diabetol Metab Syndr, 2016;8(0):1.
      Species: Human
      Sample Type: Serum
    7. Serum levels of inflammatory markers in depressed elderly patients with diabetes and mild cognitive impairment.
      Authors: Gorska-Ciebiada M, Saryusz-Wolska M, Borkowska A, Ciebiada M, Loba J
      PLoS ONE, 2015;10(3):e0120433.
      Species: Human
      Sample Type: Serum
    8. Number of sons contributes to ageing-associated inflammation.
      Authors: Marttila S, Nevalainen T, Kananen L, Jylhava J, Jylha M, Hervonen A, Ilonen J, Hurme M
      Sci Rep, 2015;5(0):8631.
      Species: Human
      Sample Type: Plasma
    9. C-reactive protein (CRP), interferon gamma-inducible protein 10 (IP-10), and lipopolysaccharide (LPS) are associated with risk of tuberculosis after initiation of antiretroviral therapy in resource-limited settings.
      Authors: Tenforde M, Gupte N, Dowdy D, Asmuth D, Balagopal A, Pollard R, Sugandhavesa P, Lama J, Pillay S, Cardoso S, Pawar J, Santos B, Riviere C, Mwelase N, Kanyama C, Kumwenda J, Hakim J, Kumarasamy N, Bollinger R, Semba R, Campbell T, Gupta A
      PLoS ONE, 2015;10(2):e0117424.
      Species: Human
      Sample Type: Plasma
    10. Effects of pitavastatin versus atorvastatin on the peripheral endothelial progenitor cells and vascular endothelial growth factor in high-risk patients: a pilot prospective, double-blind, randomized study.
      Authors: Lin L, Huang C, Chen J, Wu T, Leu H, Huang P, Chang T, Lin S, Chen J
      Cardiovasc Diabetol, 2014;13(0):111.
      Species: Human
      Sample Type: Serum
    11. The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study.
      Authors: Pisetsky D, Trace S, Brownley K, Hamer R, Zucker N, Roux-Lombard P, Dayer J, Bulik C
      Cytokine, 2014;69(1):110-5.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Prognostic value of a quantitative analysis of lipoarabinomannan in urine from patients with HIV-associated tuberculosis.
      Authors: Kerkhoff A, Wood R, Vogt M, Lawn S
      , 2014;0(0):.
      Species: Human
      Sample Type: Serum
    13. Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease.
      Authors: Ramachandran S, Venugopal A, Kutty V, A V, G D, Chitrasree V, Mullassari A, Pratapchandran N, Santosh K, Pillai M, Kartha C
      Cardiovasc Diabetol, 2014;13(0):38.
      Species: Human
      Sample Type: Plasma
    14. Plasma sCD14 as a biomarker to predict pulmonary exacerbations in cystic fibrosis.
      Authors: Quon B, Ngan D, Wilcox P, Man S, Sin D
      PLoS ONE, 2014;9(2):e89341.
      Species: Human
      Sample Type: Plasma
    15. HIV-associated tuberculosis: relationship between disease severity and the sensitivity of new sputum-based and urine-based diagnostic assays.
      Authors: Lawn S, Kerkhoff A, Vogt M, Wood R
      BMC Med, 2013;11(0):231.
      Species: Human
      Sample Type: Serum
    16. Predictive value of liver enzymes and inflammatory biomarkers for the severity of liver fibrosis stage in HIV/HCV co-infected patients.
      Authors: Charpentier C, Champenois K, Gervais A, Landman R, Joly V, Le Gac S, Larrouy L, Damond F, Brun-Vezinet F, Descamps D, Yazdanpanah Y
      PLoS ONE, 2013;8(3):e59205.
      Species: Human
      Sample Type: Plasma
    17. Characterization of the role of distinct plasma cell-free DNA species in age-associated inflammation and frailty.
      Authors: Jylhava J, Nevalainen T, Marttila S, Jylha M, Hervonen A, Hurme M
      Aging Cell, 2013;12(3):388-97.
    18. Influence of interleukin-4 gene polymorphisms and interleukin-4 serum level on susceptibility and severity of rheumatoid arthritis in Egyptian population.
      Authors: Hussein Y, El-Shal A, Rezk N, Abdel Galil S, Alzahrani S
      Cytokine, 2013;61(3):849-55.
      Species: Human
      Sample Type: Serum
    19. Elevated serum adipocyte fatty acid-binding protein concentrations are independently associated with renal dysfunction in patients with stable angina pectoris.
      Authors: Iwamoto M, Miyoshi T, Doi M
      Cardiovasc Diabetol, 2012;11(0):26.
      Species: Human
      Sample Type: Serum
    20. Plasma desmoplakin I biomarker of vascular recurrence after ischemic stroke.
      Authors: Lopez-Farre AJ, Zamorano-Leon JJ, Segura A, Mateos-Caceres PJ, Modrego J, Rodriguez-Sierra P, Calatrava L, Tamargo J, Macaya C
      J. Neurochem., 2012;121(2):314-25.
      Species: Human
      Sample Type: Plasma
    21. Cortisol responses to mental stress and the progression of coronary artery calcification in healthy men and women.
      Authors: Hamer M, Endrighi R, Venuraju SM, Lahiri A, Steptoe A
      PLoS ONE, 2012;7(2):e31356.
      Species: Human
      Sample Type: Serum
    22. Comparison of acute versus convalescent stage high-sensitivity C-Reactive protein level in predicting clinical outcome after acute ischemic stroke and impact of erythropoietin.
      Authors: Yeh KH, Tsai TH, Chai HT, Leu S, Chung SY, Chua S, Chen YL, Lin HS, Yuen CM, Yip HK
      J Transl Med, 2012;10(0):6.
      Species: Human
      Sample Type: Serum
    23. Shared monocyte subset phenotypes in HIV-1 infection and in uninfected subjects with acute coronary syndrome.
      Authors: Funderburg, Nicholas, Zidar, David A, Shive, Carey, Lioi, Anthony, Mudd, Joseph, Musselwhite, Laura W, Simon, Daniel I, Costa, Marco A, Rodriguez, Benigno, Sieg, Scott F, Lederman, Michael
      Blood, 2012;120(23):4599-608.
      Species: Human
      Sample Type: Plasma
    24. Validation and quality control of ELISAs for the use with human saliva samples.
      Authors: Jaedicke KM, Taylor JJ, Preshaw PM
      J. Immunol. Methods, 2012;377(1):62-5.
      Species: Human
      Sample Type: Saliva
    25. Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
      Authors: Cunnington, Aubrey J, Njie, Madi, Correa, Simon, Takem, Ebako N, Riley, Eleanor, Walther, Michael
      J Immunol, 2012;189(11):5336-46.
      Species: Human
      Sample Type: Plasma
    26. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
      Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
      BMC Pulm Med, 2012;12(0):3.
      Species: Human
      Sample Type: Plasma
    27. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
      Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
      Br. J. Cancer, 2011;104(1):51-9.
      Species: Human
      Sample Type: Serum
    28. Increased prevalence of albuminuria in HIV-infected adults with diabetes.
      Authors: Kim PS, Woods C, Dutcher L, Georgoff P, Rosenberg A, Mican JA, Kopp JB, Smith MA, Hadigan C
      PLoS ONE, 2011;6(9):e24610.
      Species: Human
      Sample Type: Serum
    29. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
      Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
      Diabetologia, 2011;54(10):2713-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
      Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
      Obesity (Silver Spring), 2011;19(11):2130-6.
      Species: Human
      Sample Type: Plasma
    31. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
      Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
      PLoS ONE, 2011;6(7):e22748.
      Species: Human
      Sample Type: Plasma
    32. C-reactive protein promotes acute renal inflammation and fibrosis in unilateral ureteral obstructive nephropathy in mice.
      Authors: Li ZI, Chung AC, Zhou L, Huang XR, Liu F, Fu P, Fan JM, Szalai AJ, Lan HY
      Lab. Invest., 2011;91(6):837-51.
      Species: Transgenic Mouse
      Sample Type: Serum
    33. Folic acid treatment normalizes NOS-dependence of vascular tone in the metabolic syndrome.
      Authors: Schneider MP, Schlaich MP, Harazny JM, Raff U, Ritt M, Ott C, Schmieder RE
      Obesity (Silver Spring), 2011;19(5):960-7.
      Species: Human
      Sample Type: Serum
    34. Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
      Authors: Furet JP, Kong LC, Tap J, Poitou C, Basdevant A, Bouillot JL, Mariat D, Corthier G, Dore J, Henegar C, Rizkalla S, Clement K
      Diabetes, 2010;59(12):3049-57.
      Species: Human
      Sample Type: Plasma
    35. Persistent cognitive depressive symptoms are associated with coronary artery calcification.
      Authors: Hamer M, Kivimaki M, Lahiri A
      Atherosclerosis, 2010;210(1):209-13.
      Species: Human
      Sample Type: Serum
    36. Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis.
      Authors: Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC
      Am. J. Cardiol., 2010;105(3):362-7.
      Species: Human
      Sample Type: Serum
    37. Salivary cortisol responses to mental stress are associated with coronary artery calcification in healthy men and women.
      Authors: Hamer M, O'Donnell K, Lahiri A, Steptoe A
      Eur. Heart J., 2010;31(4):424-9.
      Species: Human
      Sample Type: Serum
    38. Improvements in coronary heart disease risk indicators by alternate-day fasting involve adipose tissue modulations.
      Authors: Bhutani S, Klempel MC, Berger RA, Varady KA
      Obesity (Silver Spring), 2010;18(11):2152-9.
      Species: Human
      Sample Type: Plasma
    39. Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition.
      Authors: Bunck MC, Diamant M, Eliasson B, Corner A, Shaginian RM, Heine RJ, Taskinen MR, Yki-Jarvinen H, Smith U
      Diabetes Care, 2010;33(8):1734-7.
      Species: Human
      Sample Type: Serum
    40. Tauroursodeoxycholic Acid may improve liver and muscle but not adipose tissue insulin sensitivity in obese men and women.
      Authors: Kars M, Yang L, Gregor MF, Mohammed BS, Pietka TA, Finck BN, Patterson BW, Horton JD, Mittendorfer B, Hotamisligil GS, Klein S
      Diabetes, 2010;59(8):1899-905.
      Species: Human
      Sample Type: Plasma
    41. Reduction of elevated plasma osteopontin levels with resection of non-small-cell lung cancer.
      Authors: Blasberg JD, Pass HI, Goparaju CM, Flores RM, Lee S, Donington JS
      J. Clin. Oncol., 2010;28(6):936-41.
      Species: Human
      Sample Type: Plasma
    42. Predictors of maintaining cognitive function in older adults: the Health ABC study.
      Authors: Yaffe K, Fiocco AJ, Lindquist K, Vittinghoff E, Simonsick EM, Newman AB, Satterfield S, Rosano C, Rubin SM, Ayonayon HN, Harris TB,
      Neurology, 2009;72(23):2029-35.
      Species: Human
      Sample Type: Plasma
    43. Prospective study of physical fitness, adiposity, and inflammatory markers in healthy middle-aged men and women.
      Authors: Hamer M, Steptoe A
      Am. J. Clin. Nutr., 2009;89(1):85-9.
      Species: Human
      Sample Type: Plasma
    44. Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
      Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
      Thorax, 2008;63(12):1070-5.
      Species: Human
      Sample Type: Serum
    45. Endothelial progenitor cell levels in obese men with the metabolic syndrome and the effect of simvastatin monotherapy vs. simvastatin/ezetimibe combination therapy.
      Authors: Westerweel PE, Visseren FL, Hajer GR, Olijhoek JK, Hoefer IE, de Bree P, Rafii S, Doevendans PA, Verhaar MC
      Eur. Heart J., 2008;29(22):2808-17.
      Species: Human
      Sample Type: Serum
    46. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
      Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
      J. Leukoc. Biol., 2008;84(4):1109-19.
      Species: Human
      Sample Type: Recombinant Protein
    47. Effects of rehabilitative exercise on peripheral muscle TNFalpha, IL-6, IGF-I and MyoD expression in patients with COPD.
      Authors: Vogiatzis I, Stratakos G, Simoes DC, Terzis G, Georgiadou O, Roussos C, Zakynthinos S
      Thorax, 2007;62(11):950-6.
      Species: Human
      Sample Type: Plasma
    48. Muscle atrophy and hypertrophy signaling in patients with chronic obstructive pulmonary disease.
      Authors: Doucet M, Russell AP, Leger B, Debigare R, Joanisse DR, Caron MA, LeBlanc P, Maltais F
      Am. J. Respir. Crit. Care Med., 2007;176(3):261-9.
      Species: Human
      Sample Type: Plasma
    Expand to show all 49 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 960 for Human CRP

    Ctrl QC70
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 5 (Based on 1 review)

    Have you used Human C-Reactive Protein/CRP Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Human C-Reactive Protein/CRP Quantikine ELISA Kit.

    Filter by Ratings
    Excellent
    (1)
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
     Human C-Reactive Protein/CRP Quantikine ELISA Kit DCRP00
    Enlarge
    Excellent
      Anonymous 10/02/2017
    Show
    Image Details
     Human C-Reactive Protein/CRP Quantikine ELISA Kit DCRP00
    : Human C-Reactive Protein/CRP Quantikine ELISA Kit [DCRP00]

    Summary

    Sample TestedSerum and Plasma

    Other Experimental Details

    Other Experimental DetailsRobust and very easy to use assay

    Order Details

    Contact Information

    R&D Systems Guarantee

    Feedback

    Customer Information