Human C4.4A/LYPD3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human C4.4A/LYPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse C4.4A is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human C4.4A/LYPD3
    Leu31-His286
    Accession # O95274
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human C4.4A/LYPD3 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for C4.4A/LYPD3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C4.4A/LYPD3

LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI anchored metastasis-associated protein C4.4A) is an 85‑95 kDa, variably glycosylated member of the Ly6 family of GPI anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin‑3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296 amino acid (aa) GPI linked glycoprotein. It contains two UPAR domains
(aa 33‑126 and 140‑222), and an ADAM cleavage site (aa 312‑325). The molecule shows extensive N and O linked glycosylation. There is one potential isoform that shows a deletion of aa 129‑180. Over aa 31‑286, human LYPD3 shares 81% aa identity with mouse LYPD3.

  • Long Name:
    Leucine Rich Repeat and Fibronectin Type III Domain Containing 3
  • Entrez Gene IDs:
    27076 (Human); 72434 (Mouse); 60378 (Rat)
  • Alternate Names:
    C4.4A; C4.4A2310061G07Rik; GPI-anchored metastasis-associated protein C4.4A homolog; GPI-anchored metastasis-associated protein homolog; LY6/PLAUR domain containing 3; ly6/PLAUR domain-containing protein 3; LYPD3; Matrigel-induced gene C4 protein; MIG-C4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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