Detection of Human C4.4A/LYPD3 by Western Blot.
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for C4.4A/LYPD3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI anchored metastasis-associated protein C4.4A) is an 85‑95 kDa, variably glycosylated member of the Ly6 family of GPI anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin‑3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296 amino acid (aa) GPI linked glycoprotein. It contains two UPAR domains (aa 33‑126 and 140‑222), and an ADAM cleavage site (aa 312‑325). The molecule shows extensive N and O linked glycosylation. There is one potential isoform that shows a deletion of aa 129‑180. Over aa 31‑286, human LYPD3 shares 81% aa identity with mouse LYPD3.
Leucine Rich Repeat and Fibronectin Type III Domain Containing 3
Entrez Gene IDs:
27076 (Human); 72434 (Mouse); 60378 (Rat)
C4.4A; C4.4A2310061G07Rik; GPI-anchored metastasis-associated protein C4.4A homolog; GPI-anchored metastasis-associated protein homolog; LY6/PLAUR domain containing 3; ly6/PLAUR domain-containing protein 3; LYPD3; Matrigel-induced gene C4 protein; MIG-C4
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