Human C4.4A/LYPD3 Antibody

R&D Systems | Catalog # AF5428

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all C4.4A/LYPD3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human C4.4A/LYPD3
Leu31-His286
Accession # O95274

Specificity

Detects human C4.4A/LYPD3 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human C4.4A/LYPD3 Antibody

Detection of Human C4.4A/LYPD3 by Western Blot.

Western blot shows lysates of SK‑BR‑3 human breast cancer cell line, MCF‑7 human breast cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for C4.4A/LYPD3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human C4.4A/LYPD3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SK‑BR‑3 human breast cancer cell line, MCF‑7 human breast cancer cell line, and RT-4 human bladder carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C4.4A/LYPD3

LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI anchored metastasis-associated protein C4.4A) is an 85‑95 kDa, variably glycosylated member of the Ly6 family of GPI anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin‑3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296 amino acid (aa) GPI linked glycoprotein. It contains two UPAR domains
(aa 33‑126 and 140‑222), and an ADAM cleavage site (aa 312‑325). The molecule shows extensive N and O linked glycosylation. There is one potential isoform that shows a deletion of aa 129‑180. Over aa 31‑286, human LYPD3 shares 81% aa identity with mouse LYPD3.

Long Name

Leucine Rich Repeat and Fibronectin Type III Domain Containing 3

Alternate Names

LYPD3, MIG-C4

Entrez Gene IDs

27076 (Human); 72434 (Mouse); 60378 (Rat)

Gene Symbol

LYPD3

UniProt

O95274 (Human)

Additional C4.4A/LYPD3 Products

Product Documents for Human C4.4A/LYPD3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human C4.4A/LYPD3 Antibody

For research use only

Citations for Human C4.4A/LYPD3 Antibody

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