Human C4.4A/LYPD3 Antibody Summary
Accession # O95274
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human C4.4A/LYPD3 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for C4.4A/LYPD3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI anchored metastasis-associated protein C4.4A) is an 85‑95 kDa, variably glycosylated member of the Ly6 family of GPI anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin‑3. LYPD3 is believed to support cell migration. Mature human LYPD3 is a 296 amino acid (aa) GPI linked glycoprotein. It contains two UPAR domains
(aa 33‑126 and 140‑222), and an ADAM cleavage site (aa 312‑325). The molecule shows extensive N and O linked glycosylation. There is one potential isoform that shows a deletion of aa 129‑180. Over aa 31‑286, human LYPD3 shares 81% aa identity with mouse LYPD3.
Citation for Human C4.4A/LYPD3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells
Authors: M Görtz, U Galli, T Longerich, M Zöller, U Erb, P Schemmer
Oncol. Rep., 2017;38(5):2697-2704.
Sample Types: Whole Cells
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