Human Cadherin-11 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Cadherin-11 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-7, rhCadherin-8, rhCadherin-10, rhCadherin‑18 and rhCadherin-20 is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin‑11
  Phe23-Thr617
  Accession # AAA35622
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Endotoxin Level
  <0.10 EU per 1 μg of the antibody by the LAL method.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• Flow Cytometry
  2.5 µg/10⁶ cells
  PC‑3 human prostate cancer cell line stained in buffer containing Ca²⁺ and Mg²⁺
• Immunohistochemistry
  5-15 µg/mL
  See below
• Adhesion Blockade
  The adhesion of MDA-MB-231 human breast cancer cells (5 x 10⁴ cells/well) to immobilized Recombinant Human Cadherin-11 Fc Chimera (Catalog # 1790-CA, 20 µg/mL, 100 µL/well) was maximally inhibited (70-100%) by 30 µg/mL of the antibody.
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot click image to view larger

Detection of Human Cadherin‑11 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cadherin‑11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry click image to view larger

Cadherin‑11 in Human Prostate Cancer Tissue. Cadherin‑11 was detected in fomralin fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Cadherin‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized in the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Angst, B.D. et al. (2001) J. Cell Sci. 113:629.
  2. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
  3. Feltes, C.M. et al. (2002) Cancer Research. 62:6688.
  4. Wheelock, J.J. and K.R. Johnson (2003) Annu. Rev. Cell Dev. Biol. 19:207.
  5. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337.
  6. Pishvaian, M.J. et. al. (1999) Cancer Research 59:947.

• Entrez Gene IDs:
  1009 (Human); 12552 (Mouse)

• Alternate Names:
  CAD11OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin11; Cadherin-11; CDH11; CDHOB; OB; OB-Cadherin; Osteoblast cadherin

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