hh Human Caspase-1/ICE Quantikine ELISA Kit DCA100: R&D Systems

Human Caspase-1/ICE Quantikine ELISA Kit

(18 citations)
(3 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL)
  • Sensitivity
    1.24 pg/mL
  • Assay Range
    6.3 - 400 pg/mL (Cell Culture Supernates)
  • Specificity
    Natural and recombinant human Caspase-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC57, Quantikine Immunoassay Control Set 775 Human Caspase-1/ICE - Please Inquire
Product Summary
The Quantikine Human Caspase-1 Immunoassay is a 3 hour solid phase ELISA designed to measure Caspase-1 in cell culture supernates. It contains recombinant human Caspase-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Caspase-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Caspase-1. These results indicate that this kit can be used to determine relative mass values for natural human Caspase-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4177.722840.475.2219
Standard Deviation2.494.6211.113.86.918.1
CV%6.15.94.99.49.28.3

Recovery

The recovery of Caspase-1 spiked to three different levels throughout the range of the assay was evaluated

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 85-106
Linearity
To assess the linearity of the assay, cell culture supernate samples containing and/or spiked with high concentrations of Caspase-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Caspase-1/ICE Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Caspase-1

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

    • Entrez Gene IDs
      834 (Human);
    • Alternate Names
      CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta; Caspase1; Caspase-1; EC 3.4.22; EC 3.4.22.36; ICE; ICEP45; IL-1 beta-converting enzyme; IL1BC CASP1 nirs variant 1; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1-B converting enzyme; interleukin 1-beta convertase; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 100 µL Antiserum
    9.   Add 100 µL of Antiserum to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
    10.   Aspirate and wash 3 times.

    11. 100 µL Conjugate
    12.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
    13.   Aspirate and wash 3 times.

    14. 200 µL Substrate Solution
    15. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    16. 50 µL Stop Solution
    17. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    18 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri
      Authors: JH Kim, HJ Sohn, JK Yoo, H Kang, GS Seong, YJ Chwae, K Kim, S Park, HJ Shin
      Infect. Immun, 2016;84(9):2422-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. F-actin dampens NLRP3 inflammasome activity via Flightless-I and LRRFIP2
      Sci Rep, 2016;6(0):29834.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Resolvins Decrease Oxidative Stress Mediated Macrophage and Epithelial Cell Interaction through Decreased Cytokine Secretion.
      Authors: Cox R, Phillips O, Fukumoto J, Fukumoto I, Tamarapu Parthasarathy P, Mandry M, Cho Y, Lockey R, Kolliputi N
      PLoS ONE, 2015;10(8):e0136755.
      Species: Human
      Sample Type: Cell Lysates
    4. Cross talk between neuroregulatory molecule and monocyte: nerve growth factor activates the inflammasome.
      Authors: Datta-Mitra A, Kundu-Raychaudhuri S, Mitra A, Raychaudhuri S
      PLoS ONE, 2015;10(4):e0121626.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Aggregatibacter actinomycetemcomitans cytolethal distending toxin activates the NLRP3 inflammasome in human macrophages, leading to the release of proinflammatory cytokines.
      Authors: Shenker B, Ojcius D, Walker L, Zekavat A, Scuron M, Boesze-Battaglia K
      Infect Immun, 2015;83(4):1487-96.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Pseudomonas aeruginosa Triggers Macrophage Autophagy To Escape Intracellular Killing by Activation of the NLRP3 Inflammasome.
      Authors: Deng Q, Wang Y, Zhang Y, Li M, Li D, Huang X, Wu Y, Pu J, Wu M
      Infect Immun, 2015;84(1):56-66.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Monocyte Caspase-1 Is Released in a Stable, Active High Molecular Weight Complex Distinct from the Unstable Cell Lysate-Activated Caspase-1.
      Authors: Shamaa O, Mitra S, Gavrilin M, Wewers M
      PLoS ONE, 2015;10(11):e0142203.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. CREB-induced inflammation is important for malignant mesothelioma growth.
      Authors: Westbom C, Shukla A, MacPherson M, Yasewicz E, Miller J, Beuschel S, Steele C, Pass H, Vacek P, Shukla A
      Am J Pathol, 2014;184(10):2816-27.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Microvesicular caspase-1 mediates lymphocyte apoptosis in sepsis.
      Authors: Exline M, Justiniano S, Hollyfield J, Berhe F, Besecker B, Das S, Wewers M, Sarkar A
      PLoS ONE, 2014;9(3):e90968.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
      Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
      Blood, 2013;121(1):95-106.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1beta production and activates the NLRP3 inflammasome in human macrophages.
      Authors: Huang T, Ojcius D, Young J, Wu Y, Ko Y, Wong T, Wu C, Lu C, Lai H
      PLoS ONE, 2012;7(7):e41383.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling.
      Authors: Beynon V, Quintana FJ, Weiner HL
      PLoS ONE, 2012;7(6):e39576.
      Species: Human
      Sample Type: Serum
    13. HMG-CoA reductase inhibition induces IL-1beta release through Rac1/PI3K/PKB-dependent caspase-1 activation.
      Authors: Kuijk LM, Beekman JM, Koster J, Waterham HR, Frenkel J, Coffer PJ
      Blood, 2008;112(9):3563-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Haplotypes of the caspase-1 gene, plasma caspase-1 levels, and cardiovascular risk.
      Authors: Blankenberg S, Godefroy T, Poirier O, Rupprecht HJ, Barbaux S, Bickel C, Nicaud V, Schnabel R, Kee F, Morrison C, Evans A, Lackner KJ, Cambien F, Munzel T, Tiret L,
      Circ. Res., 2006;99(1):102-8.
      Species: Human
      Sample Type: Plasma
    15. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
      Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
      Clin. Cancer Res., 2006;12(2):376-82.
      Species: Human
      Sample Type: Plasma
    16. The effect of the physical characteristics of hydroxyapatite particles on human monocytes IL-18 production in vitro.
      Authors: Grandjean-Laquerriere A, Laquerriere P, Laurent-Maquin D, Guenounou M, Phillips TM
      Biomaterials, 2004;25(28):5921-7.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Evaluation of caspase 1 and sFas serum levels in patients with systemic sclerosis: correlation with lung dysfunction, joint and bone involvement.
      Authors: Dziankowska-Bartkowiak B, Waszczykowska E, Zalewska A, Sysa-Jedrzejowska A
      Mediators Inflamm., 2003;12(6):339-43.
      Species: Human
      Sample Type: Serum
    18. Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells: involvement of 5-hydroxytryptamine2A receptors.
      Authors: Cloez-Tayarani I, Petit-Bertron AF, Venters HD, Cavaillon JM
      Int. Immunol., 2003;15(2):233-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 18 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 775 Human Caspase-1/ICE

    Ctrl QC57
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 4.7 (Based on 3 reviews)

    Have you used Human Caspase-1/ICE Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Human Caspase-1/ICE Quantikine ELISA Kit.

    Filter by Ratings
    Excellent
    (2)
    Very Good
    (1)
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
      Very Good
      Anonymous 12/02/2017
    Show

    Summary

    Sample TestedHuman plasma
     Human Caspase-1/ICE Quantikine ELISA Kit DCA100
    Enlarge
    Excellent
      Anonymous 06/01/2017
    Show
    Image Details
     Human Caspase-1/ICE Quantikine ELISA Kit DCA100
    : Human Caspase-1/ICE Quantikine ELISA Kit [DCA100]

    Summary

    Sample TestedCell Culture Supernates
     Human Caspase-1/ICE Quantikine ELISA Kit DCA100
    Enlarge
    Excellent
      Anonymous 05/10/2016
    Show
    Image Details
     Human Caspase-1/ICE Quantikine ELISA Kit DCA100
    : Human Caspase-1/ICE Quantikine ELISA Kit [DCA100]

    Summary

    Sample TestedHuman plasma EDTA

    Order Details

    Contact Information

    R&D Systems Guarantee

    Feedback

    Customer Information