Human Caspase-1/ICE Quantikine ELISA Kit

  (20 citations)
(3 Reviews)
    
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Assay Procedure
Citations (20)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL)
  • Sensitivity
    1.24 pg/mL
  • Assay Range
    6.3 - 400 pg/mL (Cell Culture Supernates)
  • Specificity
    Natural and recombinant human Caspase-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC57 , Quantikine Immunoassay Control Set 775 Human Caspase-1/ICE - Please Inquire
Product Summary
The Quantikine Human Caspase-1 Immunoassay is a 3 hour solid phase ELISA designed to measure Caspase-1 in cell culture supernates. It contains recombinant human Caspase-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Caspase-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Caspase-1. These results indicate that this kit can be used to determine relative mass values for natural human Caspase-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 41 77.7 228 40.4 75.2 219
Standard Deviation 2.49 4.62 11.11 3.8 6.9 18.1
CV% 6.1 5.9 4.9 9.4 9.2 8.3

Recovery

The recovery of Caspase-1 spiked to three different levels throughout the range of the assay was evaluated

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 85-106
Linearity
To assess the linearity of the assay, cell culture supernate samples containing and/or spiked with high concentrations of Caspase-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Caspase-1 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Caspase-1

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

  • Entrez Gene IDs:
    834 (Human)
  • Alternate Names:
    CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta; Caspase1; Caspase-1; EC 3.4.22; EC 3.4.22.36; ICE; ICEP45; IL-1 beta-converting enzyme; IL1BC CASP1 nirs variant 1; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1-B converting enzyme; interleukin 1-beta convertase; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 100 µL Antiserum
  9.   Add 100 µL of Antiserum to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
  10.   Aspirate and wash 3 times.

  11. 100 µL Conjugate
  12.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
  13.   Aspirate and wash 3 times.

  14. 200 µL Substrate Solution
  15. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  16. 50 µL Stop Solution
  17. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Sample Type
  1. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis
    Authors: Y Han, X Xu, C Tang, P Gao, X Chen, X Xiong, M Yang, S Yang, X Zhu, S Yuan, F Liu, L Xiao, YS Kanwar, L Sun
    Redox Biol, 2018;16(0):32-46.
    Species: Human
    Sample Type: Cell Lysates
  2. Inhibition of the inflammatory response to stress by targeting interaction between PKR and its cellular activator PACT
    Authors: S Dabo, P Maillard, M Collados R, MD Hansen, S Mazouz, DJ Bigot, M Tible, G Janvier, O Helynck, P Cassonnet, Y Jacob, J Bellalou, A Gatignol, RC Patel, J Hugon, H Munier-Leh, EF Meurs
    Sci Rep, 2017;7(1):16129.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri
    Authors: JH Kim, HJ Sohn, JK Yoo, H Kang, GS Seong, YJ Chwae, K Kim, S Park, HJ Shin
    Infect. Immun, 2016;84(9):2422-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. F-actin dampens NLRP3 inflammasome activity via Flightless-I and LRRFIP2
    Sci Rep, 2016;6(0):29834.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Monocyte Caspase-1 Is Released in a Stable, Active High Molecular Weight Complex Distinct from the Unstable Cell Lysate-Activated Caspase-1.
    Authors: Shamaa O, Mitra S, Gavrilin M, Wewers M
    PLoS ONE, 2015;10(11):e0142203.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Cross talk between neuroregulatory molecule and monocyte: nerve growth factor activates the inflammasome.
    Authors: Datta-Mitra A, Kundu-Raychaudhuri S, Mitra A, Raychaudhuri S
    PLoS ONE, 2015;10(4):e0121626.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Pseudomonas aeruginosa Triggers Macrophage Autophagy To Escape Intracellular Killing by Activation of the NLRP3 Inflammasome.
    Authors: Deng Q, Wang Y, Zhang Y, Li M, Li D, Huang X, Wu Y, Pu J, Wu M
    Infect Immun, 2015;84(1):56-66.
    Species: Human
    Sample Type: Cell Culture Supernates
  8. Resolvins Decrease Oxidative Stress Mediated Macrophage and Epithelial Cell Interaction through Decreased Cytokine Secretion.
    Authors: Cox R, Phillips O, Fukumoto J, Fukumoto I, Tamarapu Parthasarathy P, Mandry M, Cho Y, Lockey R, Kolliputi N
    PLoS ONE, 2015;10(8):e0136755.
    Species: Human
    Sample Type: Cell Lysates
  9. Aggregatibacter actinomycetemcomitans cytolethal distending toxin activates the NLRP3 inflammasome in human macrophages, leading to the release of proinflammatory cytokines.
    Authors: Shenker B, Ojcius D, Walker L, Zekavat A, Scuron M, Boesze-Battaglia K
    Infect Immun, 2015;83(4):1487-96.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. Microvesicular caspase-1 mediates lymphocyte apoptosis in sepsis.
    Authors: Exline M, Justiniano S, Hollyfield J, Berhe F, Besecker B, Das S, Wewers M, Sarkar A
    PLoS ONE, 2014;9(3):e90968.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. CREB-induced inflammation is important for malignant mesothelioma growth.
    Authors: Westbom C, Shukla A, MacPherson M, Yasewicz E, Miller J, Beuschel S, Steele C, Pass H, Vacek P, Shukla A
    Am J Pathol, 2014;184(10):2816-27.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
    Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
    Blood, 2013;121(1):95-106.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling.
    Authors: Beynon V, Quintana FJ, Weiner HL
    PLoS ONE, 2012;7(6):e39576.
    Species: Human
    Sample Type: Serum
  14. The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1beta production and activates the NLRP3 inflammasome in human macrophages.
    Authors: Huang T, Ojcius D, Young J, Wu Y, Ko Y, Wong T, Wu C, Lu C, Lai H
    PLoS ONE, 2012;7(7):e41383.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. HMG-CoA reductase inhibition induces IL-1beta release through Rac1/PI3K/PKB-dependent caspase-1 activation.
    Authors: Kuijk LM, Beekman JM, Koster J, Waterham HR, Frenkel J, Coffer PJ
    Blood, 2008;112(9):3563-73.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Haplotypes of the caspase-1 gene, plasma caspase-1 levels, and cardiovascular risk.
    Authors: Blankenberg S, Godefroy T, Poirier O, Rupprecht HJ, Barbaux S, Bickel C, Nicaud V, Schnabel R, Kee F, Morrison C, Evans A, Lackner KJ, Cambien F, Munzel T, Tiret L,
    Circ. Res., 2006;99(1):102-8.
    Species: Human
    Sample Type: Plasma
  17. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006;12(2):376-82.
    Species: Human
    Sample Type: Plasma
  18. The effect of the physical characteristics of hydroxyapatite particles on human monocytes IL-18 production in vitro.
    Authors: Grandjean-Laquerriere A, Laquerriere P, Laurent-Maquin D, Guenounou M, Phillips TM
    Biomaterials, 2004;25(28):5921-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  19. Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells: involvement of 5-hydroxytryptamine2A receptors.
    Authors: Cloez-Tayarani I, Petit-Bertron AF, Venters HD, Cavaillon JM
    Int. Immunol., 2003;15(2):233-40.
    Species: Human
    Sample Type: Cell Culture Supernates
  20. Evaluation of caspase 1 and sFas serum levels in patients with systemic sclerosis: correlation with lung dysfunction, joint and bone involvement.
    Authors: Dziankowska-Bartkowiak B, Waszczykowska E, Zalewska A, Sysa-Jedrzejowska A
    Mediators Inflamm., 2003;12(6):339-43.
    Species: Human
    Sample Type: Serum
Expand to show all Citations

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Quantikine Immunoassay Control Set 775 Human Caspase-1/ICE

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Average Rating: 4.7 (Based on 3 reviews)

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  Very Good
  Anonymous 12/02/2017
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Sample Tested Human plasma
 Human Caspase-1/ICE Quantikine ELISA Kit DCA100 Array DCA100
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  Anonymous 06/01/2017
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 Human Caspase-1/ICE Quantikine ELISA Kit DCA100
: Human Caspase-1/ICE Quantikine ELISA Kit [DCA100].

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Sample Tested Cell Culture Supernates
 Human Caspase-1/ICE Quantikine ELISA Kit DCA100 Array DCA100
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  Anonymous 05/10/2016
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 Human Caspase-1/ICE Quantikine ELISA Kit DCA100
: Human Caspase-1/ICE Quantikine ELISA Kit [DCA100].

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Sample Tested Human plasma EDTA

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