Detects human CCL1/I‑309/TCA‑3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma,
11, 14, 17, 19, 20, 21, 25, 28, recombinant mouse CCL2, 3, 5, 6, 7, 9/10/MIP-1 gamma,
11, 21, or 25 is observed.
Monoclonal Mouse IgG1 Clone # 35305
Protein A or G purified from ascites
E. coli-derived recombinant human CCL1/I‑309/TCA‑3 Lys24-Lys96 Accession # P22362
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I)
Human CCL1/I-309 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human CCL1/I‑309/TCA‑3 Antibody (Catalog #
ELISA Detection (Matched Antibody Pair)
Human CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog #
Recombinant Human CCL1/I-309 Protein (Catalog #
Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.02 µg/mL Recombinant Human CCL1/I‑309/TCA‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Antibody.
Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL1/I‑309 Monoclonal Antibody (Catalog # MAB272). The ND50 is typically 0.1‑0.5 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid mature protein. The CCL1 sequence contains one potential N‑linked glycosylation site, and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.
Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Entrez Gene IDs:
6346 (Human); 20290 (Mouse)
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you
include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image.
Limited to verified customers in USA, Canada and Europe.
Read what people are saying who have used Human CCL1/I-309/TCA-3 Antibody.
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.