Human CCL1/I-309/TCA-3 Antibody

(2 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL1/I‑309/TCA‑3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 11, 14, 17, 19, 20, 21, 25, 28, recombinant mouse CCL2, 3, 5, 6, 7, 9/10/MIP-1 gamma, 11, 21, or 25 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 35305
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL1/I‑309/TCA‑3
    Lys24-Lys96
    Accession # P22362
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I)
    • Human CCL1/I-309 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CCL1/I‑309/TCA‑3 Antibody (Catalog # MAB272)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog # BAF272)
  • ELISA Standard
     
    Recombinant Human CCL1/I-309 Protein (Catalog # 272-I)
  • Neutralization
    Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.02 µg/mL Recombinant Human CCL1/I‑309/TCA‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Anti­body. Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL1/I‑309 Monoclonal Antibody (Catalog # MAB272). The ND50 is typically
0.1‑0.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid mature protein. The CCL1 sequence contains one potential N‑linked glycosylation site, and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

  • References:
    1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
    2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.
  • Entrez Gene IDs:
    6346 (Human); 20290 (Mouse)
  • Alternate Names:
    C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. CC chemokine ligand 1 is released into the airways of atopic asthmatics.
    Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
    Eur. Respir. J., 2006;28(1):59-67.
    Species: Human
    Sample Type: BALF
    Application: ELISA Development
  2. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: ELISA.

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ELISA Human CCL1/I-309/TCA-3 Antibody MAB272
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 ELISA Human Anonymous 11/22/2017
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ELISA Human CCL1/I-309/TCA-3 Antibody MAB272
ELISA: Human CCL1/I-309/TCA-3 Antibody [MAB272]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsMAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable.

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