Human CCL15/MIP‑1 delta Biotinylated Antibody

Catalog #: BAF363
1 Citation Datasheet / COA / SDS

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL15/MIP‑1 delta Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL15/MIP-1δ in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human MPIF‑1, recombinant mouse (rm) C10, and rmMIP-1 gamma is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CCL15/MIP-1δ (R&D Systems, Catalog # 363-MG)
Gln22-Ile113
Accession # Q16663

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CCL15/MIP‑1δ 92 aa (Catalog # 363-MG)

Human CCL15/MIP-1δ Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human CCL15/MIP-1 delta Antibody (Catalog # MAB363)

Standard: Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein (Catalog # 363-MG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL15/MIP-1 delta

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

References

Wang, W. et al. (1998) J. Clinical Immunol. 18:214

Entrez Gene IDs

6359 (Human)

Alternate Names

CCL15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP1 delta; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human CCL15/MIP‑1 delta Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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