Human CCL15/MIP-1 delta Antibody Summary
Accession # Q16663.2
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Human CCL15/MIP-1δ Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL15/MIP‑1δ and Neutral-ization by Human CCL15/ MIP‑1δ Antibody. Recombinant Human CCL15/ MIP-1d (Catalog # 363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP-1d (16 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP-1d Mono-clonal Antibody (Catalog # MAB363). The ND50 is typically 3-15 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL15/MIP-1 delta
CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.
Citations for Human CCL15/MIP-1 delta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Expression and regulation of CCL15 by human airway smooth muscle cells.
Authors: Joubert P, Lajoie-Kadoch S, Wellemans V, LÃƒÂ©tuvÃƒÂ© S, Tulic MK, Halayko AJ, Hamid Q
Clin. Exp. Allergy, 2011;42(1):85-94.
Sample Types: Whole Cells
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Sample Types: Urine
Applications: Antibody Array Development
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
Ann. Rheum. Dis., 2006;65(3):294-300.
Sample Types: Whole Tissue
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