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Human CCL15/MIP-1 delta Antibody

Catalog #: MAB363
3 Citations Datasheet / COA / SDS
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MAB363
MAB363-SP

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Chemotaxis Induced by CCL15/MIP‑1δ and Neutral-ization by Human CCL15/ MIP‑1δ Antibody.
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Citations (3)
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Human CCL15/MIP-1 delta Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL15/MIP‑1δ in ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 52130
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CCL15/MIP-1δ
Gln22-Ile113
Accession # Q16663.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL15/MIP‑1δ 92 aa (Catalog # 363-MG)

Human CCL15/MIP-1δ Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL15/MIP‑1 delta Biotinylated Antibody (Catalog # BAF363)

Standard: Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein (Catalog # 363-MG)

Neutralization
Measured by its ability to neutralize CCL15/MIP‑1δ-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 16 ng/mL Recombinant Human CCL15/MIP‑1δ 92 aa.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL15/MIP‑1δ and Neutral-ization by Human CCL15/ MIP‑1δ Antibody. View Larger

Chemotaxis Induced by CCL15/MIP‑1δ and Neutral-ization by Human CCL15/ MIP‑1δ Antibody. Recombinant Human CCL15/ MIP-1d (Catalog # 363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP-1d (16 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP-1d Mono-clonal Antibody (Catalog # MAB363). The ND50 is typically 3-15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL15/MIP-1 delta

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

References
  1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214
Entrez Gene IDs
6359 (Human)
Alternate Names
CCL15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP1 delta; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15

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Citations for Human CCL15/MIP-1 delta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Expression and regulation of CCL15 by human airway smooth muscle cells.
    Authors: Joubert P, Lajoie-Kadoch S, Wellemans V, Létuvé S, Tulic MK, Halayko AJ, Hamid Q
    Clin. Exp. Allergy, 2011-11-09;42(1):85-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  3. Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
    Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
    Ann. Rheum. Dis., 2005-08-17;65(3):294-300.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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