Human CCL17/TARC Antibody

Catalog # Availability Size / Price Qty
MAB364-SP
MAB364-100
MAB364-500
Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody.
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Product Details
Citations (11)
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Supplemental Products
Reviews (1)

Human CCL17/TARC Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL17/TARC in ELISAs. Does not cross-react with recombinant human (rh) CCL2, 3, 4, 5, 7, 8, 11, 13, 16, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL2, 3, 4, 5, 7, 9/10/MIP-1 gamma, 11, 12, 21, 22, 25, or rrCCL20.
Source
Monoclonal Mouse IgG1 Clone # 54026
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL17/TARC
Ala24-Ser94
Accession # Q92583.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample

Human CCL17/TARC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL17/TARC Biotinylated Antibody (Catalog # BAF364)

Standard: Recombinant Human CCL17/TARC Protein (Catalog # 364-DN)

Neutralization
Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 10 ng/mL Recombinant Human CCL17/TARC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody. View Larger

Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody. Recombinant Human CCL17/TARC (Catalog # 364-DN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/TARC (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL17/TARC Monoclonal Antibody (Catalog # MAB364). The ND50 is typically 0.4-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL17/TARC

CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

References
  1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
  2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
  3. Nomiyama, H. et al. (1997) Genomics 40:211.
Entrez Gene IDs
6361 (Human); 20295 (Mouse)
Alternate Names
ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine

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Citations for Human CCL17/TARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. A high-throughput chemotaxis detection method for CCR4+ T cell migration inhibition using image cytometry
    Authors: Elizabeth L. Magnotti, Leo Li-Ying Chan, Quan Zhu, Wayne A. Marasco
    Journal of Immunological Methods
  2. HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk
    Authors: Yingying Wang, Xiaoling Weng, Luoyang Wang, Mingang Hao, Yue Li, Lidan Hou et al.
    Journal of Clinical Investigation
  3. CMT2D neuropathy is linked to the neomorphic binding activity of glycyl-tRNA synthetase
    Authors: Weiwei He, Ge Bai, Huihao Zhou, Na Wei, Nicholas M. White, Janelle Lauer et al.
    Nature
  4. Lung transplantation affects expression of the chemokine receptor type 4 on specific T cell subsets.
    Authors: Paantjens AW, van de Graaf EA, Kwakkel-van Erp JM, Hoefnagel T, van Kessel DA, van den Bosch JM, Otten HG
    Clin. Exp. Immunol., 2011-10-01;166(1):103-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer.
    Authors: Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H
    Int. J. Cancer, 2008-05-15;122(10):2286-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis.
    Authors: Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B
    Blood, 2007-10-29;111(2):761-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007-10-01;179(7):4840-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. TARC augments TNF-alpha-induced CTACK production in keratinocytes.
    Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
    Exp. Dermatol., 2004-09-01;13(9):551-7.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Neutralization
  9. Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival.
    Authors: Curiel, Tyler J, Coukos, George, Zou, Linhua, Alvarez, Xavier, Cheng, Pui, Mottram, Peter, Evdemon-Hogan, Melina, Conejo-Garcia, Jose R, Zhang, Lin, Burow, Matthew, Zhu, Yun, Wei, Shuang, Kryczek, Ilona, Daniel, Ben, Gordon, Alan, Myers, Leann, Lackner, Andrew, Disis, Mary L, Knutson, Keith L, Chen, Lieping, Zou, Weiping
    Nat Med, 2004-08-22;10(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
    Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
    Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  11. Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.
    Authors: Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN
    J. Immunol., 2001-06-01;166(11):6899-906.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: ELISA Development

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Human CCL17/TARC Antibody
By Anonymous on 11/22/2017
Application: ELISA Sample Tested: Cell culture supernatant,Serum and Plasma Species: Human

The TARC ELISA we made used MAB364 as the capture and BAF364 as the detection with recombinant antige 364-DN as the immunoassay standard.