CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Transgenic Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Neutralization, Bioassay, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 54026
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Product Specifications
Immunogen
E. coli-derived recombinant human CCL17/TARC
Ala24-Ser94
Accession # Q92583.1
Ala24-Ser94
Accession # Q92583.1
Specificity
Detects human CCL17/TARC in ELISAs. Does not cross-react with recombinant human
(rh) CCL2, 3, 4, 5, 7, 8, 11, 13, 16, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL2, 3,
4, 5, 7, 9/10/MIP-1 gamma, 11, 12, 21, 22, 25, or rrCCL20.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL17/TARC Antibody
Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody.
Recombinant Human CCL17/TARC (Catalog # 364-DN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/TARC (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL17/TARC Monoclonal Antibody (Catalog # MAB364). The ND50 is typically 0.4-2.0 µg/mL.Applications for Human CCL17/TARC Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 10 ng/mL Recombinant Human CCL17/TARC.
Human CCL17/TARC Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB364 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL17/TARC
References
- Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
- Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
- Nomiyama, H. et al. (1997) Genomics 40:211.
Alternate Names
ABCD-2, SCYA17, TARC
Gene Symbol
CCL17
UniProt
Additional CCL17/TARC Products
Product Documents for Human CCL17/TARC Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CCL17/TARC Antibody
For research use only
Citations for Human CCL17/TARC Antibody
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Customer Reviews for Human CCL17/TARC Antibody (1)
5 out of 5
1 Customer Rating
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Application: ELISASample Tested: Cell culture supernatant and Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/22/2017The TARC ELISA we made used MAB364 as the capture and BAF364 as the detection with recombinant antige 364-DN as the immunoassay standard.
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Associated Pathways
Th2 Differentiation Pathway
