CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.
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Human CCL17/TARC Quantikine ELISA Kit
R&D Systems | Catalog # DDN00
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Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
7 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human CCL17/TARC Quantikine ELISA Kit
The Quantikine Human TARC Immunoassay kit is a 3.5 hour solid phase ELISA designed to measure TARC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TARC and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TARC. Results obtained using natural human TARC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TARC.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human TARC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human TARC in this assay. No medical histories were available for the donors used in this
study.
*Two serum samples and one EDTA plasma sample measured substantially higher and are not included
in this range.
ND=Non-detectable
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Serume (n=66)* | 331 | 71-848 |
| EDTA plasma (n=34)* | 134 | 35-285 |
| Heparin plasma (n=35) | 111 | 33-226 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106
cells/mL)
were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were
cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates
were removed on days 1 and 5 and assayed for levels of human TARC.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | ND | 1880 |
| Stimulated | 1336 | 1696 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 88 | 504 | 1001 | 89 | 540 | 1033 |
| Standard Deviation | 4.1 | 21.7 | 27.2 | 8.3 | 38.9 | 71.7 |
| CV% | 4.7 | 4.3 | 2.7 | 9.3 | 7.2 | 6.9 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 87 | 600 | 1107 | 98 | 616 | 1178 |
| Standard Deviation | 6.2 | 13.7 | 29.9 | 8.9 | 50.7 | 88.8 |
| CV% | 7.1 | 2.3 | 2.7 | 9.1 | 8.2 | 7.5 |
Recovery for Human CCL17/TARC Quantikine ELISA Kit
The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 88-104 |
| EDTA Plasma (n=5) | 102 | 86-111 |
| Heparin Plasma (n=5) | 98 | 86-107 |
| Serum (n=5) | 105 | 100-114 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TARC were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL17/TARC Quantikine ELISA Kit
Human CCL17/TARC ELISA Calibrator Diluent RD5-5 Standard Curve
Human CCL17/TARC ELISA Calibrator Diluent RD6Q Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL17/TARC
Additional CCL17/TARC Products
Product Documents for Human CCL17/TARC Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CCL17/TARC Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human CCL17/TARC Quantikine ELISA Kit
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Sample Tested: SerumVerified Customer | Posted 06/27/2019Importance of serum „Thymus and Activation-Regulated Chemokine (TARC) determination in classical Hodgkin lymphoma’s (cHL) patients Thymus and Activation-Regulated Chemokine (TARC or CCL17) is highly expressed by Hodgkin and Reed-Sternberg cells and secreted into the serum. Therefore it can be a useful tumor marker of classical Hodgkin lymphoma (cHL). Our aim has been to study the value of serum TARC concentrations in the course of treatment, and follow up in patients with cHL. We enrolled 613 patients diagnosed with cHL, who were treated by the National Institute of Oncology, Hungary, between 2011 and 2018. Serum TARC levels were determined with a quantitative sandwich enzyme immunoassay technique (RnD Systems, Biotechne, Minneapolis, MN). Measurements were carried out in 3 month intervals following diagnosis, and 6 month intervals after 3 years. In 241 cases, serum TARC concentrations were determined before therapy, as well. Total number of measurements taken were 2015. Receiver Operating Characteristic (ROC) curve analysis was performed for comparison of healthy controls (n=168) and patients with active disease status before treatment (n=241). The cut-off value was 637 pg/ml with 90.9% sensitivity and 98.2% specificity. During follow-ups the median serum TARC value was 14350 pg/ml in patients with active disease status. In case of complete remission it was 401 pg/ml. ROC curve analysis of active versus complete remission groups showed a high 85.6% sensitivity and 93.7% specificity with a 936 pg/ml cut-off value. Our data confirmed the usefulness of TARC determination in the course of treatment and follow-up. TARC may be a potential marker for early response assessment and may be able to predict potential relapse of the disease. Determination of serum TARC concentrations with high sensitivity and cost-efficacy, is highly recommened for monitoring and at diagnosis of cHL patients.
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Protocols
View specific protocols for Human CCL17/TARC Quantikine ELISA Kit (DDN00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
- Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Th2 Differentiation Pathway
