Human CCL17/TARC Quantikine ELISA Kit

(29 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    7 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human TARC
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human TARC Immunoassay kit is a 3.5 hour solid phase ELISA designed to measure TARC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TARC and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TARC. Results obtained using natural human TARC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TARC.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4.121.727.28.338.971.7

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation6.213.729.98.950.788.8


The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 88-104
EDTA Plasma (n=5) 102 86-111
Heparin Plasma (n=5) 98 86-107
Serum (n=5) 105 100-114
To assess the linearity of the assay, samples containing or spiked with high concentrations of TARC were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL17/TARC Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL17/TARC
CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.
    • Entrez Gene IDs
      6361 (Human); 20295 (Mouse);
    • Alternate Names
      ABCD-2; CC chemokine TARC; C-C motif chemokine 17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 29
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    Sample Type
    1. Prognostic significance of pretreatment serum cytokines in classical Hodgkin lymphoma.
      Authors: Marri P, Hodge L, Maurer M, Ziesmer S, Slager S, Habermann T, Link B, Cerhan J, Novak A, Ansell S
      Clin Cancer Res, 2013;19(24):6812-9.
      Species: Human
      Sample Type: Serum
    2. Regulatory T cells suppress T cell activation at the pathologic site of human visceral leishmaniasis.
      Authors: Rai AK, Thakur CP, Singh A
      PLoS ONE, 2012;7(2):e31551.
      Species: Human
      Sample Type: Serum
    3. Mepolizumab as a corticosteroid-sparing agent in lymphocytic variant hypereosinophilic syndrome.
      Authors: Roufosse F, de Lavareille A, Schandene L, Cogan E, Georgelas A, Wagner L, Xi L, Raffeld M, Goldman M, Gleich GJ, Klion A
      J. Allergy Clin. Immunol., 2010;126(4):828-835.
      Species: Human
      Sample Type: Serum
    4. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
      Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
      J. Immunol., 2010;184(6):2886-98.
    5. Brentuximab vedotin (SGN-35) for relapsed CD30-positive lymphomas.
      Authors: Younes A, Bartlett NL, Leonard JP
      N. Engl. J. Med., 2010;363(19):1812-21.
      Species: Human
      Sample Type: Serum
    6. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
      Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
      Mediators Inflamm., 2009;2009(0):269541.
      Species: Human
      Sample Type: Serum
    7. Increases in serum TARC/CCL17 levels are associated with progression-free survival in advanced melanoma patients in response to dendritic cell-based immunotherapy.
      Authors: Cornforth AN, Lee GJ, Fowler AW, Carbonell DJ, Dillman RO
      J. Clin. Immunol., 2009;29(5):657-64.
      Species: Human
      Sample Type: Serum
    8. Phenotype of atopic dermatitis subjects with a history of eczema herpeticum.
      Authors: Beck LA, Boguniewicz M, Hata T, Schneider LC, Hanifin J, Gallo R, Paller AS, Lieff S, Reese J, Zaccaro D, Milgrom H, Barnes KC, Leung DY
      J. Allergy Clin. Immunol., 2009;124(2):260-9, 269.e1.
      Species: Human
      Sample Type: Serum
    9. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
      Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
      J. Interferon Cytokine Res., 2008;28(5):297-310.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
      Authors: Miller R, Sadhukhan R, Wu C
      J. Immunol. Methods, 2008;334(1):134-41.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
      Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
      Blood, 2007;111(4):2339-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
      Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
      Am. J. Respir. Crit. Care Med., 2007;177(1):44-55.
      Species: Human
      Sample Type: BALF
    13. Comparison of serum markers for allergic bronchopulmonary aspergillosis in cystic fibrosis.
      Authors: Latzin P, Hartl D, Regamey N, Frey U, Schoeni MH, Casaulta C
      Eur. Respir. J., 2007;31(1):36-42.
      Species: Human
      Sample Type: Serum
    14. Circulating thymus- and activation-regulated chemokine/CCL17 is a useful biomarker for discriminating acute eosinophilic pneumonia from other causes of acute lung injury.
      Authors: Miyazaki E, Nureki S, Ono E, Ando M, Matsuno O, Fukami T, Ueno T, Kumamoto T
      Chest, 2007;131(6):1726-34.
      Species: Human
      Sample Type: BALF
    15. Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis.
      Authors: Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B
      Blood, 2007;111(2):761-6.
      Species: Human
      Sample Type: CSF
    16. CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
      Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
      J. Immunol., 2006;176(10):5760-71.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
      Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
      Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
      Species: Human
      Sample Type: Serum
    18. Der p, IL-4, and TGF-beta cooperatively induce EGFR-dependent TARC expression in airway epithelium.
      Authors: Heijink IH, Marcel Kies P, van Oosterhout AJ, Postma DS, Kauffman HF, Vellenga E
      Am. J. Respir. Cell Mol. Biol., 2006;36(3):351-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Glucocorticoid-induced tumour necrosis factor receptor (GITR) and its ligand (GITRL) in atopic dermatitis.
      Authors: Baumgartner-Nielsen J, Vestergaard C, Thestrup-Pedersen K
      Acta Derm. Venereol., 2006;86(5):393-8.
      Species: Human
      Sample Type: Plasma
    20. Serum levels of cutaneous T-cell attracting chemokine (CTACK) as a laboratory marker of the severity of atopic dermatitis in children.
      Authors: Hon KL, Leung TF, Ma KC, Li AM, Wong Y, Fok TF
      Clin. Exp. Dermatol., 2004;29(3):293-6.
      Species: Human
      Sample Type: Serum
    21. CSF chemokine levels in relapsing neuromyelitis optica and multiple sclerosis.
      Authors: Narikawa K, Misu T, Fujihara K, Nakashima I, Sato S, Itoyama Y
      J. Neuroimmunol., 2004;149(1):182-6.
      Species: Human
      Sample Type: Serum
    22. Increased macrophage-derived chemokine in exhaled breath condensate and plasma from children with asthma.
      Authors: Leung TF, Wong GW, Ko FW, Lam CW, Fok TF
      Clin. Exp. Allergy, 2004;34(5):786-91.
      Species: Human
      Sample Type: Plasma
    23. Helper T-lymphocyte-related chemokines in healthy newborns.
      Authors: Leung TF, Ng PC, Tam WH, Li CY, Wong E, Ma TP, Lam CW, Fok TF
      Pediatr. Res., 2003;55(2):334-8.
      Species: Human
      Sample Type: Serum
    24. Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma.
      Authors: Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K
      Cytokine, 2003;23(4):126-32.
      Species: Human
      Sample Type: Serum
    25. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
      Authors: D&amp;amp;apos;Ambrosio D
      Eur. J. Immunol., 2002;32(10):2934-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. Elevated levels of thymus and activation-regulated chemokine (TARC) in pleural effusion samples from patients infested with Paragonimus westermani.
      Authors: Matsumoto N, Mukae H, Nakamura-Uchiyama F, Ashitani JI, Abe K, Katoh S, Kohno S, Nawa Y, Matsukura S
      Clin. Exp. Immunol., 2002;130(2):314-8.
      Species: Human
      Sample Type: Pleural Effusion
    27. Characterization of monocyte subtypes in the allergic form of atopic eczema/dermatitis syndrome.
      Authors: Novak N, Allam P, Geiger E, Bieber T
      Allergy, 2002;57(10):931-5.
      Species: Human
      Sample Type: Serum
    28. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
      Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
      J. Leukoc. Biol., 2001;70(4):617-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
      Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
      Eur. J. Immunol., 2001;31(4):1037-46.
      Species: Human
      Sample Type: Serum
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