Human CCL18/PARC Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
Control Products Available
Human CCL18/PARC Simple Plex Assay Linearity
3 Images
Product Details
Citations (10)
Supplemental Products

Human CCL18/PARC Quantikine ELISA Kit Summary

Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Tissue Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)
1.77 pg/mL
Assay Range
18.8 - 1,200 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Natural and recombinant human CCL18.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CCL18/PARC Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human CCL18 in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human CCL18 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CCL18 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CCL18.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 70.8 248 592 84.2 265 614
Standard Deviation 2.27 8.21 21.8 5.1 17.1 27.6
CV% 3.2 3.3 3.7 6.1 6.5 4.5


The recovery of human CCL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 91-110
Tissue Lysis Buffer (n=1) 92 91-93
Urine (n=4) 104 92-112


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human CCL18 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human CCL18/PARC Simple Plex Assay Linearity
Human CCL18/PARC ELISA Linearity

Scientific Data

Human CCL18/PARC ELISA Standard Curve

Product Datasheets

You must select a language.


Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL18/PARC

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

Entrez Gene IDs:
6362 (Human)
Alternate Names:
Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Citations for Human CCL18/PARC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma
    Authors: J Wang, N Wang, Z Zheng, Y Che, M Suzuki, S Kano, J Lu, P Wang, Y Sun, A Homma
    BMC Cancer, 2022;22(1):1208.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
    Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
    Cell Reports, 2022;39(2):110670.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype
    Authors: W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang
    Oncogene, 2022;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Improving risk-stratification of rheumatoid arthritis patients for interstitial lung disease
    Authors: J Avouac, A Cauvet, A Steelandt, Y Shirai, M Elhai, M Kuwana, O Distler, Y Allanore
    PLoS ONE, 2020;15(5):e0232978.
    Species: Human
    Sample Types: Serum
  5. M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations
    Authors: A Nikonova, M Khaitov, DJ Jackson, S Traub, MB Trujillo-T, DA Kudlay, AS Dvornikov, A Del-Rosari, R Valenta, LA Stanciu, R Khaitov, SL Johnston
    EBioMedicine, 2020;54(0):102734.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. The use of patient-derived breast tissue explants to study macrophage polarization and the effects of environmental chemical exposure
    Authors: KJ Gregory, SM Morin, A Kubosiak, J Ser-Dolans, B Schalet, DJ Jerry, SS Schneider
    Immunol. Cell Biol., 2020;0(0):.
    Species: Human
    Sample Types: cell culture supernatant
  7. Repeated 5-day cycles of low dose aldesleukin in amyotrophic lateral sclerosis (IMODALS): A phase 2a randomised, double-blind, placebo-controlled trial
    Authors: W Camu, M Mickunas, JL Veyrune, C Payan, C Garlanda, M Locati, R Juntas-Mor, N Pageot, A Malaspina, U Andreasson, J Kirby, C Suehs, S Saker, C Masseguin, J De Vos, H Zetterberg, PJ Shaw, A Al-Chalabi, PN Leigh, T Tree, G Bensimon
    EBioMedicine, 2020;0(0):102844.
    Species: Human
    Sample Types: Plasma
  8. IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages
    Authors: EA Ayaub, K Tandon, M Padwal, J Imani, H Patel, A Dubey, O Mekhael, C Upagupta, A Ayoub, A Dvorkin-Gh, J Murphy, PS Kolb, S Lhotak, JG Dickhout, RC Austin, MR Kolb, CD Richards, K Ask
    Immunol. Cell Biol., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Inflammation-regulating factors in ascites as predictive biomarkers of drug resistance and progression-free survival in serous epithelial ovarian cancers.
    Authors: Lane D, Matte I, Garde-Granger P, Laplante C, Carignan A, Rancourt C, Piche A
    BMC Cancer, 2015;15(0):492.
    Species: Human
    Sample Types: Peritoneal Fluid
  10. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
    Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
    BMC Pulm Med, 2012;12(0):3.
    Species: Human
    Sample Types: Plasma


No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Human CCL18/PARC Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human CCL18/PARC Quantikine ELISA Kit and earn rewards!

Have you used Human CCL18/PARC Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review