Human CCL20/MIP-3 alpha Quantikine ELISA Kit

(29 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.87 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human MIP-3 alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human MIP-3 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure MIP-3 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-3 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-3 alpha.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1.624.289.16.0517.935.3

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1.843.8315.14.511328.8


The recovery of MIP-3 alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 101 88-115
EDTA Plasma (n=5) 104 97-115
Heparin Plasma (n=5) 104 98-112
Serum (n=5) 103 94-112
To assess the linearity of the assay, samples containing or spiked with high concentrations of MIP-3 alpha in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL20/MIP-3 alpha Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL20/MIP-3 alpha
Macrophage inflammatory protein 3 alpha (MIP-3 alpha), also known as LARC (liver and activation-regulated chemokine) and as exodus-1, is one of many novel CC chemokines identified through bioinformatics. MIP-3 alpha is distantly related to other CC chemokines (20 - 28% amino acid sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% amino acid sequence identity with mouse and human MIP-3 alpha, respectively.
    • Entrez Gene IDs
      6364 (Human); 20297 (Mouse); 29538 (Rat);
    • Alternate Names
      beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 29
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    Sample Type
    1. CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
      Authors: Li W, Liu H
      Scand J Immunol, 2015;83(1):33-7.
      Species: Human
      Sample Type: Tissue Homogenates
    2. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
      Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
      Autophagy, 2014;10(2):257-68.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Selective impact of HIV disease progression on the innate immune system in the human female reproductive tract.
      Authors: Lahey T, Ghosh M, Fahey JV, Shen Z, Mukura LR, Song Y, Cu-Uvin S, Mayer KH, Wright PF, Kappes JC, Ochsenbauer C, Wira CR
      PLoS ONE, 2012;7(6):e38100.
      Species: Human
      Sample Type: cervicovaginal lavage
    4. A randomized trial to assess anti-HIV activity in female genital tract secretions and soluble mucosal immunity following application of 1% tenofovir gel.
      Authors: Keller MJ, Madan RP, Torres NM
      PLoS ONE, 2011;6(1):e16475.
      Species: Human
      Sample Type: cervicovaginal lavage
    5. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
      Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
      Clin. Cancer Res., 2011;17(24):7529-38.
      Species: Human
      Sample Type: Serum
    6. Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
      Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
      Eur. J. Immunol., 2010;40(9):2450-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
      Authors: Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
      J. Invest. Dermatol., 2010;130(6):1646-56.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
      Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
      Allergy, 2010;65(5):581-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
      Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
      J. Immunol., 2009;182(0):3112-20.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
      Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
      J. Exp. Med., 2009;206(1):169-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis.
      Authors: Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A
      J. Invest. Dermatol., 2009;129(0):2175.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
      Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
      J. Exp. Med., 2009;206(3):535-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
      Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
      Cancer Res., 2009;69(15):6249-55.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
      Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
      Mediators Inflamm., 2009;2009(0):269541.
      Species: Human
      Sample Type: Serum
    15. Urinary fractalkine is a marker of acute rejection.
      Authors: Peng W, Chen J, Jiang Y, Wu J, Shou Z, He Q, Wang Y, Chen Y, Wang H
      Kidney Int., 2008;74(11):1454-60.
      Species: Human
      Sample Type: Urine
    16. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
      Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
      J. Neuroimmunol., 2008;199(1):35-45.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Macrophage inflammatory protein-3alpha is a novel serum marker for nasopharyngeal carcinoma detection and prediction of treatment outcomes.
      Authors: Chang KP, Hao SP, Chang JH, Wu CC, Tsang NM, Lee YS, Hsu CL, Ueng SH, Liu SC, Liu YL, Wei PC, Liang Y, Chang YS, Yu JS
      Clin. Cancer Res., 2008;14(21):6979-87.
      Species: Human
      Sample Type: Serum
    18. Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
      Authors: Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG
      Am. J. Pathol., 2008;173(1):195-204.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus.
      Authors: Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M
      Arthritis Rheum., 2008;58(10):3205-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli.
      Authors: Real JM, Munro P, Buisson-Touati C, Lemichez E, Boquet P, Landraud L
      Cytokine, 2007;37(1):22-5.
      Species: Human
      Sample Type: Urine
    21. Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease.
      Authors: Demedts IK, Bracke KR, Van Pottelberge G, Testelmans D, Verleden GM, Vermassen FE, Joos GF, Brusselle GG
      Am. J. Respir. Crit. Care Med., 2007;175(10):998-1005.
      Species: Human
      Sample Type: Sputum
    22. Airway dendritic cell phenotypes in inflammatory diseases of the human lung.
      Authors: Lommatzsch M, Bratke K, Bier A, Julius P, Kuepper M, Luttmann W, Virchow JC
      Eur. Respir. J., 2007;30(5):878-86.
      Species: Human
      Sample Type: BALF
    23. Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene.
      Authors: Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L
      J. Immunol., 2006;177(3):1941-55.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells.
      Authors: Fichorova RN, Trifonova RT, Gilbert RO, Costello CE, Hayes GR, Lucas JJ, Singh BN
      Infect. Immun., 2006;74(10):5773-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Expression of CCL20 and granulocyte-macrophage colony-stimulating factor, but not Flt3-L, from modified vaccinia virus ankara enhances antiviral cellular and humoral immune responses.
      Authors: Chavan R, Marfatia KA, An IC, Garber DA, Feinberg MB
      J. Virol., 2006;80(15):7676-87.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
      Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
      J. Exp. Med., 2006;203(12):2763-77.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells.
      Authors: Peterson ML, Schlievert PM
      Biochemistry, 2006;45(7):2387-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma.
      Authors: Parameswaran K, Liang H, Fanat A, Watson R, Snider DP, O&amp;amp;apos;Byrne PM
      J. Allergy Clin. Immunol., 2004;114(1):73-9.
      Species: Human
      Sample Type: Sputum
    29. Plasticity of airway epithelial cell transcriptome in response to flagellin.
      Authors: Clark J, Kim K, Basom R, Gharib S
      PLoS ONE, 0;10(2):e0115486.
      Species: Human
      Sample Type: Cell Culture Supernates
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    Quantikine Immunoassay Control Set 804 Human CCL20/MIP-3a

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    Quantikine Wash Buffer 1

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