Human CCL20/MIP-3 alpha Quantikine ELISA Kit

  (33 citations)
(2 Reviews)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (33)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.87 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human MIP-3 alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC124 , Quantikine Immunoassay Control Set 804 Human CCL20/MIP-3a - Please Inquire
Product Summary
The Quantikine Human MIP-3 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure MIP-3 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-3 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-3 alpha.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 66.2 180 372 68.7 186 357
Standard Deviation 1.84 3.83 15.1 4.51 13 28.8
CV% 2.8 2.1 4.1 6.6 6.9 8.1

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 55.9 161 323 72.5 200 386
Standard Deviation 1.62 4.28 9.1 6.05 17.9 35.3
CV% 2.9 2.7 2.8 8.3 8.9 9.1

Recovery

The recovery of MIP-3 alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 101 88-115
EDTA Plasma (n=5) 104 97-115
Heparin Plasma (n=5) 104 98-112
Serum (n=5) 103 94-112
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of MIP-3 alpha in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 CCL20/MIP-3 alpha [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL20/MIP-3 alpha
Macrophage inflammatory protein 3 alpha (MIP-3 alpha), also known as LARC (liver and activation-regulated chemokine) and as exodus-1, is one of many novel CC chemokines identified through bioinformatics. MIP-3 alpha is distantly related to other CC chemokines (20 - 28% amino acid sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% amino acid sequence identity with mouse and human MIP-3 alpha, respectively.
  • Entrez Gene IDs:
    6364 (Human); 20297 (Mouse); 29538 (Rat)
  • Alternate Names:
    beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy
    Authors: G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang
    PLoS ONE, 2017;12(5):e0178352.
    Species: Human
    Sample Type: Serum
  2. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. CCL20 mediates RANK/RANKL-induced epithelial-mesenchymal transition in endometrial cancer cells
    Oncotarget, 2016;7(18):25328-39.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma.
    Authors: Li W, Liu H
    Scand J Immunol, 2015;83(1):33-7.
    Species: Human
    Sample Type: Tissue Homogenates
  5. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
    Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
    Autophagy, 2014;10(2):257-68.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Selective impact of HIV disease progression on the innate immune system in the human female reproductive tract.
    Authors: Lahey T, Ghosh M, Fahey JV, Shen Z, Mukura LR, Song Y, Cu-Uvin S, Mayer KH, Wright PF, Kappes JC, Ochsenbauer C, Wira CR
    PLoS ONE, 2012;7(6):e38100.
    Species: Human
    Sample Type: Vaginal Lavage
  7. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Type: Serum
  8. A randomized trial to assess anti-HIV activity in female genital tract secretions and soluble mucosal immunity following application of 1% tenofovir gel.
    Authors: Keller MJ, Madan RP, Torres NM
    PLoS ONE, 2011;6(1):e16475.
    Species: Human
    Sample Type: Vaginal Lavage
  9. Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
    Authors: Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
    J. Invest. Dermatol., 2010;130(6):1646-56.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
    Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
    Eur. J. Immunol., 2010;40(9):2450-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
    Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
    Allergy, 2010;65(5):581-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis.
    Authors: Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A
    J. Invest. Dermatol., 2009;129(0):2175.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009;2009(0):269541.
    Species: Human
    Sample Type: Serum
  14. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
    Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
    J. Exp. Med., 2009;206(3):535-48.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009;182(0):3112-20.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  18. Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus.
    Authors: Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M
    Arthritis Rheum., 2008;58(10):3205-15.
    Species: Human
    Sample Type: Cell Culture Supernates
  19. Urinary fractalkine is a marker of acute rejection.
    Authors: Peng W, Chen J, Jiang Y, Wu J, Shou Z, He Q, Wang Y, Chen Y, Wang H
    Kidney Int., 2008;74(11):1454-60.
    Species: Human
    Sample Type: Urine
  20. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
    Authors: Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG
    Am. J. Pathol., 2008;173(1):195-204.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Macrophage inflammatory protein-3alpha is a novel serum marker for nasopharyngeal carcinoma detection and prediction of treatment outcomes.
    Authors: Chang KP, Hao SP, Chang JH, Wu CC, Tsang NM, Lee YS, Hsu CL, Ueng SH, Liu SC, Liu YL, Wei PC, Liang Y, Chang YS, Yu JS
    Clin. Cancer Res., 2008;14(21):6979-87.
    Species: Human
    Sample Type: Serum
  23. IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
    Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
    J. Immunol., 2008;180(1):655-63.
    Species: Human
    Sample Type: Cell Culture Supernates
  24. Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli.
    Authors: Real JM, Munro P, Buisson-Touati C, Lemichez E, Boquet P, Landraud L
    Cytokine, 2007;37(1):22-5.
    Species: Human
    Sample Type: Urine
  25. Airway dendritic cell phenotypes in inflammatory diseases of the human lung.
    Authors: Lommatzsch M, Bratke K, Bier A, Julius P, Kuepper M, Luttmann W, Virchow JC
    Eur. Respir. J., 2007;30(5):878-86.
    Species: Human
    Sample Type: BALF
  26. Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease.
    Authors: Demedts IK, Bracke KR, Van Pottelberge G, Testelmans D, Verleden GM, Vermassen FE, Joos GF, Brusselle GG
    Am. J. Respir. Crit. Care Med., 2007;175(10):998-1005.
    Species: Human
    Sample Type: Sputum
  27. Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells.
    Authors: Peterson ML, Schlievert PM
    Biochemistry, 2006;45(7):2387-97.
    Species: Human
    Sample Type: Cell Culture Supernates
  28. Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells.
    Authors: Fichorova RN, Trifonova RT, Gilbert RO, Costello CE, Hayes GR, Lucas JJ, Singh BN
    Infect. Immun., 2006;74(10):5773-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  29. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Human
    Sample Type: Cell Culture Supernates
  30. Expression of CCL20 and granulocyte-macrophage colony-stimulating factor, but not Flt3-L, from modified vaccinia virus ankara enhances antiviral cellular and humoral immune responses.
    Authors: Chavan R, Marfatia KA, An IC, Garber DA, Feinberg MB
    J. Virol., 2006;80(15):7676-87.
    Species: Human
    Sample Type: Cell Culture Supernates
  31. Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene.
    Authors: Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L
    J. Immunol., 2006;177(3):1941-55.
    Species: Human
    Sample Type: Cell Culture Supernates
  32. Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma.
    Authors: Parameswaran K, Liang H, Fanat A, Watson R, Snider DP, O&amp;amp;apos;Byrne PM
    J. Allergy Clin. Immunol., 2004;114(1):73-9.
    Species: Human
    Sample Type: Sputum
  33. Plasticity of airway epithelial cell transcriptome in response to flagellin.
    Authors: Clark J, Kim K, Basom R, Gharib S
    PLoS ONE, 0;10(2):e0115486.
    Species: Human
    Sample Type: Cell Culture Supernates
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 804 Human CCL20/MIP-3a

Ctrl QC124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 2 reviews)

Have you used Human CCL20/MIP-3 alpha Quantikine ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human CCL20/MIP-3 alpha Quantikine ELISA Kit.

Filter by Ratings
Excellent
(2)
All Ratings
Filter by Applications
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
 Human CCL20/MIP-3 alpha Quantikine ELISA Kit DM3A00 Array DM3A00
Enlarge
Excellent
  Anonymous 01/20/2017
Show
Image Details
 Human CCL20/MIP-3 alpha Quantikine ELISA Kit DM3A00
: Human CCL20/MIP-3 alpha Quantikine ELISA Kit [DM3A00].

Summary

Sample Tested Cell Culture Supernates

Other Experimental Details

Other Experimental Details This kit is a very nice tool in determining the level of MIP-3alpha in the cell culture spent media.
  Excellent
  Anonymous 01/03/2017
Show

Summary

Sample Tested Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)

Other Experimental Details

Other Experimental Details This kit is an excellent tool in determining the level of MIP-3a in the culture media.

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee