Human CCL20/MIP-3 alpha APC-conjugated Antibody
Human CCL20/MIP-3 alpha APC-conjugated Antibody Summary
Ala27-Met96 (Asn95Asp)
Accession # P78556.1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CCL20/MIP‑3 alpha in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes either (A) untreated or (B) treated with LPS were stained with Mouse Anti-Human CCL20/MIP-3a APC-conjugated Monoclonal Antibody (Catalog # IC360A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL20/MIP-3 alpha
CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.
CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.
- Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
- Hromas, R. et al. (1997) Blood 89:3315.
- Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Product Datasheets
Citation for Human CCL20/MIP-3 alpha APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines
Authors: Parween, F;Singh, SP;Kathuria, N;Zhang, HH;Ashida, S;Otaizo-Carrasquero, FA;Shamsaddini, A;Gardina, PJ;Ganesan, S;Kabat, J;Lorenzi, HA;Riley, DJ;Myers, TG;Pittaluga, S;Bielekova, B;Farber, JM;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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