Human CCL20/MIP-3 alpha APC-conjugated Antibody

IC360A has been discontinued. View all CCL20/MIP-3 alpha products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CCL19, rhCXCL1, rhCCL16, rhCCL11, recombinant mouse CCL20, or recombinant rat CCL20.
  • Source
    Monoclonal Mouse IgG1 Clone # 67310
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL20/MIP-3 alpha
    Ala27-Met96 (Asn95Asp)
    Accession # P78556.1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of CCL20/MIP‑3 alpha in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes either (A) untreated or (B) treated with LPS were stained with Mouse Anti-Human CCL20/MIP‑3 alpha APC‑conjugated Monoclonal Antibody (Catalog # IC360A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL20/MIP-3 alpha

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  • References:
    1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
    2. Hromas, R. et al. (1997) Blood 89:3315.
    3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
  • Entrez Gene IDs:
    6364 (Human); 20297 (Mouse); 29538 (Rat)
  • Alternate Names:
    beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38
Related Research Areas

Have you used Human CCL20/MIP-3 alpha APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information